Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   DXE41_RS04435 Genome accession   NZ_LT992475
Coordinates   791961..793031 (+) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus isolate 20_LA_415     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 786961..798031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE41_RS04405 - 787384..788847 (+) 1464 WP_031795511.1 rhomboid family protein -
  DXE41_RS04410 - 788828..789031 (+) 204 WP_000087559.1 YqgQ family protein -
  DXE41_RS04415 - 789028..790014 (+) 987 WP_000161315.1 ROK family glucokinase -
  DXE41_RS04420 - 790014..790343 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DXE41_RS04425 - 790340..790963 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DXE41_RS04430 comGA 791015..791989 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DXE41_RS04435 comGB 791961..793031 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  DXE41_RS04440 comGC 793045..793356 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DXE41_RS04445 comGD 793334..793780 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  DXE41_RS04450 comGE 793767..794066 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  DXE41_RS04455 comGF 793984..794481 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  DXE41_RS04460 - 794578..794724 (+) 147 WP_001789879.1 hypothetical protein -
  DXE41_RS04465 - 794714..795238 (+) 525 WP_001015120.1 shikimate kinase -
  DXE41_RS04470 gcvT 795397..796488 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DXE41_RS04475 gcvPA 796508..797854 (+) 1347 WP_031795510.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=1150037 DXE41_RS04435 WP_000776425.1 791961..793031(+) (comGB) [Staphylococcus aureus isolate 20_LA_415]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1150037 DXE41_RS04435 WP_000776425.1 791961..793031(+) (comGB) [Staphylococcus aureus isolate 20_LA_415]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment