Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   DXE66_RS11195 Genome accession   NZ_LT992474
Coordinates   2222805..2223875 (-) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus isolate 19_LA_388     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2217805..2228875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE66_RS11155 gcvPA 2217982..2219328 (-) 1347 WP_031795510.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DXE66_RS11160 gcvT 2219348..2220439 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DXE66_RS11165 - 2220598..2221122 (-) 525 WP_001015120.1 shikimate kinase -
  DXE66_RS11170 - 2221112..2221258 (-) 147 WP_001789879.1 hypothetical protein -
  DXE66_RS11175 comGF 2221355..2221852 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  DXE66_RS11180 comGE 2221770..2222069 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DXE66_RS11185 comGD 2222056..2222502 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  DXE66_RS11190 comGC 2222480..2222791 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DXE66_RS11195 comGB 2222805..2223875 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  DXE66_RS11200 comGA 2223847..2224821 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DXE66_RS11205 - 2224873..2225496 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DXE66_RS11210 - 2225493..2225822 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DXE66_RS11215 - 2225822..2226808 (-) 987 WP_000161315.1 ROK family glucokinase -
  DXE66_RS11220 - 2226805..2227008 (-) 204 WP_000087559.1 YqgQ family protein -
  DXE66_RS11225 - 2226989..2228452 (-) 1464 WP_031795511.1 rhomboid family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=1150000 DXE66_RS11195 WP_000776425.1 2222805..2223875(-) (comGB) [Staphylococcus aureus isolate 19_LA_388]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1150000 DXE66_RS11195 WP_000776425.1 2222805..2223875(-) (comGB) [Staphylococcus aureus isolate 19_LA_388]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment