Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   DXE64_RS00935 Genome accession   NZ_LT992470
Coordinates   183203..184273 (+) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus isolate 13_LA_301     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 178203..189273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE64_RS00905 - 178626..180089 (+) 1464 WP_031795511.1 rhomboid family protein -
  DXE64_RS00910 - 180070..180273 (+) 204 WP_000087559.1 YqgQ family protein -
  DXE64_RS00915 - 180270..181256 (+) 987 WP_000161315.1 ROK family glucokinase -
  DXE64_RS00920 - 181256..181585 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DXE64_RS00925 - 181582..182205 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DXE64_RS00930 comGA 182257..183231 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DXE64_RS00935 comGB 183203..184273 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  DXE64_RS00940 comGC 184287..184598 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DXE64_RS00945 comGD 184576..185022 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  DXE64_RS00950 comGE 185009..185308 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  DXE64_RS00955 comGF 185226..185723 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  DXE64_RS00960 - 185820..185966 (+) 147 WP_001789879.1 hypothetical protein -
  DXE64_RS00965 - 185956..186480 (+) 525 WP_001015120.1 shikimate kinase -
  DXE64_RS00970 gcvT 186639..187730 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DXE64_RS00975 gcvPA 187750..189096 (+) 1347 WP_031795510.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=1149807 DXE64_RS00935 WP_000776425.1 183203..184273(+) (comGB) [Staphylococcus aureus isolate 13_LA_301]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1149807 DXE64_RS00935 WP_000776425.1 183203..184273(+) (comGB) [Staphylococcus aureus isolate 13_LA_301]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment