Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LSAJ64_RS06125 Genome accession   NZ_LT960781
Coordinates   1197392..1198099 (-) Length   235 a.a.
NCBI ID   WP_226474265.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain J64     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1192392..1203099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSAJ64_RS06100 (LSAJ64_1222) rpsO 1192687..1192956 (-) 270 WP_011374766.1 30S ribosomal protein S15 -
  LSAJ64_RS06105 (LSAJ64_1223) rpsT 1193235..1193489 (+) 255 WP_011374767.1 30S ribosomal protein S20 -
  LSAJ64_RS06110 (LSAJ64_1224) holA 1193557..1194585 (-) 1029 WP_025015735.1 DNA polymerase III subunit delta -
  LSAJ64_RS06115 (LSAJ64_1225) comEC 1194582..1196648 (-) 2067 WP_231953244.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LSAJ64_RS06120 (LSAJ64_1226) - 1196846..1197334 (-) 489 WP_011374770.1 ComE operon protein 2 -
  LSAJ64_RS06125 (LSAJ64_1227) comEA 1197392..1198099 (-) 708 WP_226474265.1 helix-hairpin-helix domain-containing protein Machinery gene
  LSAJ64_RS06130 (LSAJ64_1228) - 1198163..1199215 (-) 1053 WP_101377905.1 SepM family pheromone-processing serine protease -
  LSAJ64_RS06135 (LSAJ64_1229) coaD 1199217..1199714 (-) 498 WP_025015732.1 pantetheine-phosphate adenylyltransferase -
  LSAJ64_RS06140 (LSAJ64_1230) rsmD 1199727..1200281 (-) 555 WP_016265217.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  LSAJ64_RS06145 (LSAJ64_1231) - 1200286..1200576 (-) 291 WP_016265218.1 YlbG family protein -
  LSAJ64_RS06150 (LSAJ64_1232) - 1200600..1201688 (-) 1089 WP_025015731.1 CAP-associated domain-containing protein -
  LSAJ64_RS06155 (LSAJ64_1233) - 1201699..1202886 (-) 1188 WP_025015730.1 FtsW/RodA/SpoVE family cell cycle protein -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26122.75 Da        Isoelectric Point: 9.7683

>NTDB_id=1148395 LSAJ64_RS06125 WP_226474265.1 1197392..1198099(-) (comEA) [Latilactobacillus sakei strain J64]
MMWQDYWQRYRRFWWVPCLVLLGIITGGFYWHQTQQKQKVTHNEQLLATSASKIKPATKKTHQAATKKKGGFVDIKGAVA
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTPEQASTG
TSANASSASDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=1148395 LSAJ64_RS06125 WP_226474265.1 1197392..1198099(-) (comEA) [Latilactobacillus sakei strain J64]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCACGGG
TGGCTTTTATTGGCATCAAACGCAACAAAAACAAAAAGTAACGCATAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
TAAAACCTGCTACTAAGAAAACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGTGCTGTGGCT
TATCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCCCAAAAATTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATTGCAACGACCAGTGAGCGTACGCCAGAACAAGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCGCGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

97.021

100

0.97

Multiple sequence alignment