Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   CKW02_RS05320 Genome accession   NZ_LT906438
Coordinates   1069443..1072952 (+) Length   1169 a.a.
NCBI ID   WP_003210760.1    Uniprot ID   -
Organism   Bacillus pumilus strain NCTC10337     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1064443..1077952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW02_RS05290 (SAMEA4076707_01063) lepB 1064963..1065475 (+) 513 WP_003212294.1 signal peptidase I -
  CKW02_RS05295 (SAMEA4076707_01064) - 1065510..1066655 (-) 1146 WP_003211629.1 AbrB family transcriptional regulator -
  CKW02_RS05300 - 1066922..1067336 (+) 415 Protein_968 BlaI/MecI/CopY family transcriptional regulator -
  CKW02_RS05305 (SAMEA4076707_01067) - 1067329..1068168 (+) 840 WP_003212081.1 M56 family metallopeptidase -
  CKW02_RS05310 (SAMEA4076707_01068) - 1068224..1068634 (+) 411 WP_003211332.1 DoxX family protein -
  CKW02_RS05315 (SAMEA4076707_01069) - 1068831..1069277 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  CKW02_RS05320 (SAMEA4076707_01070) addB 1069443..1072952 (+) 3510 WP_003210760.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  CKW02_RS05325 (SAMEA4076707_01071) addA 1072930..1076628 (+) 3699 WP_003212309.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  CKW02_RS05330 (SAMEA4076707_01072) sbcD 1076655..1077830 (+) 1176 WP_003210926.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134733.56 Da        Isoelectric Point: 5.3447

>NTDB_id=1147065 CKW02_RS05320 WP_003210760.1 1069443..1072952(+) (addB) [Bacillus pumilus strain NCTC10337]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLSQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLYAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLAKECGADISENIFERNHRHLYTPDLAYLEHQYEHRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IASPLYQLQKRLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDDEQLKFVANEH
VSLSYTISQLQQWLNQYPISGVWWSVYNYLMTSPHRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKLSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=1147065 CKW02_RS05320 WP_003210760.1 1069443..1072952(+) (addB) [Bacillus pumilus strain NCTC10337]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
ACTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGTCTTGCTTGGTCGATTTTACAGCAGACTGGT
GGAGCGAATCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCAGAGGATATTGAGAAGATTTCAGCGTCTAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTTCTTTCCCAGCAAATTCCTTCAGCTGAAGAAATAAAAGGAGCACACATCTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATCGAACAGCTCTTGCTGTATGCAGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGGAGCTGACATTTCTGAAAATATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCATCGGCCCGTTCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATCCTTGATTTAGTGAGAGAAAAAGGACTCAGACTGCGAGATATCTCGAT
TGTAGCACGGCATGTAGATGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTAATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTTGAAAACTATTGCATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGTT
TCCAATCATTAGATGAGGATTTCGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGCGTCCCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAAAGCGCAAAAGGTAAGGGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCCTTATTCCAACAGATGATAGATACAGGGTTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATTGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTACGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGTCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGAGAATGGTTAAAACGAGCAGGAGCAGAGCTAGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTGTATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAGCTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACCATCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATCAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTCATCGGGATGTATCAAAAAACATCATGTCAAGCTTGTTCTTTACAAATCGAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAGTTCAATGCG
TGTGCATTCTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATCGG
CCAATTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTTCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAATTGAG
TGGATTTTCTCCGGTAGGGCTTGAGTTAGGTTTTGGAGGTCAAGGGCCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTTGTCGGAAGGATTGACAGGGTAGATAAGGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCACTTGCAGAGGATGAAATTGAGCAGGAAATCTTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCGCAAGTCATTCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCAGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.236

99.316

0.658

Multiple sequence alignment