Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   VCBK1071_RS20990 Genome accession   NZ_LT897797
Coordinates   229712..230572 (-) Length   286 a.a.
NCBI ID   WP_088128993.1    Uniprot ID   -
Organism   Vibrio cholerae isolate Vibrio cholerae str. BC1071     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 224712..235572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCBK1071_RS01105 (VCBK1071_00211) gspL 224970..226181 (+) 1212 WP_001288754.1 type II secretion system protein GspL -
  VCBK1071_RS01110 (VCBK1071_00212) - 226191..226688 (+) 498 WP_000661348.1 type II secretion system protein M -
  VCBK1071_RS01115 (VCBK1071_00213) - 226690..227448 (+) 759 WP_000817153.1 type II secretion system protein N -
  VCBK1071_RS01120 (VCBK1071_00214) cysQ 227500..228327 (-) 828 WP_000106799.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  VCBK1071_RS01125 (VCBK1071_00215) nudE 228350..228898 (-) 549 WP_033928929.1 ADP compounds hydrolase NudE -
  VCBK1071_RS01130 (VCBK1071_00216) nfuA 229037..229624 (-) 588 WP_000070178.1 Fe-S biogenesis protein NfuA -
  VCBK1071_RS20990 comF 229712..230572 (-) 861 WP_088128993.1 ComF family protein Machinery gene
  VCBK1071_RS01140 (VCBK1071_00218) bioH 230508..231269 (+) 762 WP_172440131.1 pimeloyl-ACP methyl ester esterase BioH -
  VCBK1071_RS01145 (VCBK1071_00219) - 231431..231901 (+) 471 WP_043989980.1 hypothetical protein -
  VCBK1071_RS01150 (VCBK1071_00220) - 231996..234317 (-) 2322 WP_000030080.1 Tex family protein -
  VCBK1071_RS01155 (VCBK1071_00221) greB 234562..235044 (+) 483 WP_000850494.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32470.47 Da        Isoelectric Point: 8.0586

>NTDB_id=1146684 VCBK1071_RS20990 WP_088128993.1 229712..230572(-) (comF) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
MPQPCTNTRSCPCPLTCQYNAMVTVSPSISIFIHSANWQDEAHYPDSWLAMLTHWLRKTTAPLLIPECHLCRLALDTNSP
FGVCSACKAWLEHGYRCARCGLPTLTPVDQCGQCLGQAPPWRKLMCVGDYRFPLSDAVHQLKYQRQFWQAPRLAKLLATQ
INEPAPLLCSVPLHWQRRWLRGFNQSDLLARELANVLNIEYDHQLFARRRATPHQQGLSKAQRIHNLRDAFVLNHPPNQP
HVAIVDDVVTTGSTIRHLCDLLLDVGVQSIDIYCICRTPEPKDSHG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1146684 VCBK1071_RS20990 WP_088128993.1 229712..230572(-) (comF) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
ATGCCCCAGCCGTGTACCAATACCAGATCTTGCCCTTGTCCACTGACTTGCCAATACAACGCCATGGTCACAGTTTCTCC
GTCTATTTCCATCTTCATCCACTCTGCCAATTGGCAAGATGAAGCACATTACCCCGATTCTTGGCTCGCTATGTTAACCC
ATTGGCTCAGAAAAACTACCGCACCACTGCTCATTCCTGAGTGCCATCTTTGTCGACTCGCGCTCGATACAAATTCGCCA
TTTGGCGTCTGCTCTGCTTGCAAAGCTTGGCTTGAGCACGGTTATCGCTGCGCACGCTGCGGTTTACCGACTCTTACCCC
TGTTGATCAGTGCGGGCAGTGTTTGGGTCAGGCTCCGCCGTGGCGAAAACTGATGTGTGTCGGCGATTACCGCTTTCCAC
TTAGTGATGCGGTGCATCAACTCAAATACCAACGCCAGTTTTGGCAAGCGCCGCGCTTGGCTAAGCTACTCGCCACCCAG
ATTAACGAGCCAGCCCCGTTGCTGTGCAGTGTACCGCTACATTGGCAGCGACGTTGGCTACGCGGCTTTAATCAAAGCGA
TCTGCTGGCGCGCGAGTTAGCCAATGTGCTGAACATTGAATATGACCACCAACTCTTTGCACGCCGCCGAGCCACACCGC
ATCAACAAGGACTCAGCAAGGCGCAGCGAATCCACAACCTACGTGATGCGTTTGTGCTGAATCATCCGCCAAACCAACCA
CATGTTGCGATTGTGGACGATGTGGTGACCACAGGCAGTACGATCCGACATTTATGCGATTTACTGCTTGATGTCGGTGT
GCAAAGCATTGATATTTACTGCATATGCCGCACTCCCGAGCCGAAAGATAGCCACGGTTGA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(189-281)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Vibrio cholerae strain A1552

97.552

100

0.976
  comF Vibrio campbellii strain DS40M4

46.639

83.217

0.388

Multiple sequence alignment