Detailed information NTG0114629

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: QI18_RS09385
• Genome accession: NZ_CP010050
• Coordinates: 1912668..1913447 (-)
• Length: 259 a.a.
• NCBI ID: WP_050428201.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1907668..1918447

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QI18_RS09355 (QI18_09355)	-	1908461..1908862 (-)	402	WP_185280440.1	hypothetical protein	-
QI18_RS09360 (QI18_09360)	-	1908859..1909059 (-)	201	WP_039115873.1	DUF1655 domain-containing protein	-
QI18_RS12845 (QI18_09365)	-	1909407..1909583 (-)	177	WP_039115875.1	helix-turn-helix domain-containing protein	-
QI18_RS09370 (QI18_09370)	-	1909728..1910702 (+)	975	WP_039115877.1	helix-turn-helix domain-containing protein	-
QI18_RS09375 (QI18_09375)	-	1910782..1911981 (+)	1200	WP_039115879.1	tyrosine-type recombinase/integrase	-
QI18_RS09380 (QI18_09380)	-	1912128..1912577 (+)	450	WP_025017176.1	GNAT family N-acetyltransferase	-
QI18_RS09385 (QI18_09385)	endA	1912668..1913447 (-)	780	WP_050428201.1	DNA/RNA non-specific endonuclease	Machinery gene
QI18_RS09390 (QI18_09390)	-	1913641..1914537 (-)	897	WP_039115881.1	peptidoglycan amidohydrolase family protein	-
QI18_RS09395 (QI18_09395)	-	1914649..1914903 (-)	255	WP_023189062.1	phage holin	-
QI18_RS09400 (QI18_09400)	-	1914893..1915183 (-)	291	WP_124175219.1	hypothetical protein	-
QI18_RS09405 (QI18_09405)	-	1915400..1915693 (+)	294	WP_025017162.1	hypothetical protein	-
QI18_RS12505	-	1915723..1917963 (-)	2241	WP_052230930.1	glycoside hydrolase domain-containing protein	-
QI18_RS09415 (QI18_09415)	-	1918063..1918410 (-)	348	WP_023189066.1	DUF722 domain-containing protein	-

Sequence

Protein

• Length: 259 a.a.
• Molecular weight: 29129.05 Da
• Isoelectric Point: 9.0583

>NTDB_id=114629 QI18_RS09385 WP_050428201.1 1912668..1913447(-) (endA) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
MKNKKIIPLLIVSIFILGGCANSQSDMDKSNHKWSQKDKTPSEELAKSVLTSSIKEQLKTDNIKFDHGSFIVNNNNNDLA
IINVVDPYAKNEIDIQGRPYLGEAVLPKSIQNNEKDNKDEKVIPQFGWGQKSNLKGFYKKAYTKGFLLNSKLIGGVKDFN
DSGSNMENIFTQTVWASEANDSSSKGQKYFENMIHKVQDKNMIIKYRVKALYDGSNLVPSGTEIEAKSDDFSLEIHVFIP
NVQPNLKVDYKTGQVTEVK

Nucleotide

• Length: 780 bp

>NTDB_id=114629 QI18_RS09385 WP_050428201.1 1912668..1913447(-) (endA) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
ATGAAGAACAAAAAAATTATCCCATTACTGATTGTCAGCATTTTTATCTTAGGGGGCTGTGCTAACAGTCAATCTGATAT
GGATAAAAGTAATCATAAATGGAGTCAAAAAGATAAGACACCCTCAGAAGAACTCGCAAAAAGTGTTCTGACTTCTTCAA
TAAAAGAACAATTAAAAACAGATAACATAAAATTTGATCACGGTTCATTTATCGTTAATAATAATAACAATGATTTAGCA
ATTATTAATGTTGTAGACCCATACGCTAAGAATGAAATAGATATTCAAGGGCGTCCTTATTTGGGTGAAGCAGTTCTCCC
AAAATCTATTCAAAATAATGAAAAGGATAATAAAGATGAAAAAGTGATTCCTCAATTTGGTTGGGGACAAAAATCTAACT
TGAAAGGCTTCTATAAAAAAGCTTATACTAAAGGTTTTTTGCTAAATAGCAAACTAATAGGTGGCGTTAAAGATTTCAAT
GATAGCGGGTCAAATATGGAAAATATATTTACTCAAACGGTTTGGGCGAGTGAAGCTAATGATTCAAGTTCCAAGGGACA
AAAATATTTTGAAAATATGATTCATAAAGTTCAAGATAAGAATATGATCATCAAGTATAGAGTGAAAGCACTCTATGATG
GAAGTAACCTTGTCCCATCAGGAACTGAAATAGAAGCAAAATCTGATGATTTTTCACTCGAGATACATGTTTTTATTCCA
AATGTTCAACCAAATCTTAAAGTAGATTATAAAACAGGTCAGGTCACTGAAGTCAAATAA

Domains

Predicted by InterProScan.

• DNA/RNA non-specific endonuclease (Endonuclea_NS_2) (92-229)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	42.174	88.803	0.375
endA	Streptococcus pneumoniae D39	42.174	88.803	0.375
endA	Streptococcus pneumoniae R6	42.174	88.803	0.375
endA	Streptococcus pneumoniae TIGR4	42.174	88.803	0.375