Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NMG84_RS16880 Genome accession   NZ_LR882963
Coordinates   3686402..3687493 (+) Length   363 a.a.
NCBI ID   WP_042154823.1    Uniprot ID   A0A1J1JFB7
Organism   Planktothrix agardhii strain No.66     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3681402..3692493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMG84_RS16865 (PANO66_03375) - 3682243..3682767 (+) 525 WP_254032520.1 spore coat protein U domain-containing protein -
  NMG84_RS16870 (PANO66_03376) - 3682827..3683588 (+) 762 WP_254032521.1 molecular chaperone -
  NMG84_RS16875 (PANO66_03377) - 3683679..3686213 (+) 2535 WP_235757835.1 fimbria/pilus outer membrane usher protein -
  NMG84_RS16880 (PANO66_03378) pilM 3686402..3687493 (+) 1092 WP_042154823.1 type IV pilus biogenesis protein PilM Machinery gene
  NMG84_RS16885 (PANO66_03379) - 3687538..3688377 (+) 840 WP_027249915.1 PilN domain-containing protein -
  NMG84_RS16890 (PANO66_03380) - 3688374..3689162 (+) 789 WP_042154821.1 pilus assembly protein PilO -
  NMG84_RS16895 (PANO66_03381) - 3689283..3691613 (+) 2331 WP_254032522.1 type IV pilus secretin family protein -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 39594.45 Da        Isoelectric Point: 4.3029

>NTDB_id=1132950 NMG84_RS16880 WP_042154823.1 3686402..3687493(+) (pilM) [Planktothrix agardhii strain No.66]
MVSLLQGLFSKRKPGVGIELATERINLVELRKKGNQLKLVTLATTPVPEGIVQDGQIVDTPAMAELIQSTVTDNNIKAKT
VGTSIPGREAVTRIIPVPAELNDDELKEYMNAESGLYLPFPREEGDVDYQKLGRFVDEDGIEKVQVLLVATRKEVTDTYV
ETFAQAGLKVTVLEVSSFSLIRTLKHQLEQFSSTEAAIIADIQFDSTELAIVVNGIPQFNRAIPIGLFQVQSALNSAMNL
PPTRDISELQAMTLPVTDTMGALNDPNPGTNAIIKVFAELADELRRSVDFYINQSDGLEMAQLLLTGPGAAIGQLDEFFM
QRLAMPATQVDPIESLALAFEREIPPEQRASLGVALGLGMRMT

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=1132950 NMG84_RS16880 WP_042154823.1 3686402..3687493(+) (pilM) [Planktothrix agardhii strain No.66]
ATGGTTAGTTTATTACAAGGATTGTTCTCCAAGCGGAAGCCAGGAGTCGGAATTGAATTAGCCACAGAGCGAATTAATCT
GGTGGAACTGCGGAAAAAAGGCAACCAATTAAAGTTAGTCACCTTAGCGACCACTCCGGTTCCCGAGGGCATTGTCCAAG
ATGGCCAAATTGTCGATACTCCAGCCATGGCGGAGTTAATTCAGTCCACTGTCACCGATAACAATATCAAAGCTAAGACC
GTTGGTACATCCATCCCCGGACGGGAAGCCGTAACCCGGATTATTCCGGTTCCCGCCGAACTCAATGACGACGAACTCAA
AGAATACATGAACGCCGAGTCGGGATTGTACCTCCCATTTCCCAGGGAAGAAGGAGACGTCGATTATCAAAAATTAGGGC
GATTTGTAGATGAGGATGGCATCGAAAAAGTACAGGTTTTATTAGTCGCCACCCGCAAAGAAGTGACGGATACCTATGTA
GAAACCTTTGCTCAAGCAGGTTTAAAAGTTACTGTATTAGAAGTTAGTAGTTTTTCTCTGATTCGTACCCTAAAACATCA
ACTTGAGCAGTTTTCCTCCACCGAAGCCGCGATTATTGCCGATATTCAATTTGATAGTACGGAATTGGCGATTGTTGTCA
ATGGCATTCCCCAGTTTAACCGCGCCATTCCTATTGGTCTGTTCCAAGTGCAAAGTGCCTTAAATAGTGCCATGAATTTA
CCTCCTACGAGGGATATCAGTGAATTACAGGCCATGACCCTTCCCGTGACTGATACAATGGGAGCATTAAACGATCCGAA
TCCCGGAACCAATGCCATCATTAAGGTTTTCGCCGAATTAGCCGATGAACTACGTCGCTCCGTTGACTTTTATATTAATC
AAAGTGATGGCTTAGAAATGGCGCAGTTACTATTAACTGGCCCCGGGGCTGCGATTGGACAACTCGATGAGTTTTTCATG
CAACGGTTGGCCATGCCTGCCACCCAAGTTGACCCGATTGAATCCCTCGCCCTTGCCTTTGAGCGGGAAATTCCCCCAGA
ACAACGTGCTAGTTTAGGAGTGGCATTAGGTTTAGGAATGCGAATGACCTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-361)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J1JFB7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

54.521

100

0.548

Multiple sequence alignment