Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NMG85_RS16945 Genome accession   NZ_LR882950
Coordinates   3686590..3687681 (+) Length   363 a.a.
NCBI ID   WP_042154823.1    Uniprot ID   A0A1J1JFB7
Organism   Planktothrix agardhii strain PCC7805     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3681590..3692681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMG85_RS16935 (PCC7805_03406) - 3682326..3683633 (-) 1308 WP_227350804.1 ABC transporter substrate-binding protein -
  NMG85_RS16940 (PCC7805_03407) - 3683984..3686401 (+) 2418 WP_254034746.1 fimbria/pilus outer membrane usher protein -
  NMG85_RS16945 (PCC7805_03408) pilM 3686590..3687681 (+) 1092 WP_042154823.1 type IV pilus biogenesis protein PilM Machinery gene
  NMG85_RS16950 (PCC7805_03409) - 3687750..3688565 (+) 816 WP_233427970.1 PilN domain-containing protein -
  NMG85_RS16955 (PCC7805_03410) - 3688562..3689350 (+) 789 WP_042154821.1 pilus assembly protein PilO -
  NMG85_RS16960 (PCC7805_03411) - 3689471..3691018 (+) 1548 WP_254034747.1 AMIN domain-containing protein -
  NMG85_RS16965 (PCC7805_03412) - 3691000..3691797 (+) 798 WP_254034748.1 type II and III secretion system protein -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 39594.45 Da        Isoelectric Point: 4.3029

>NTDB_id=1132917 NMG85_RS16945 WP_042154823.1 3686590..3687681(+) (pilM) [Planktothrix agardhii strain PCC7805]
MVSLLQGLFSKRKPGVGIELATERINLVELRKKGNQLKLVTLATTPVPEGIVQDGQIVDTPAMAELIQSTVTDNNIKAKT
VGTSIPGREAVTRIIPVPAELNDDELKEYMNAESGLYLPFPREEGDVDYQKLGRFVDEDGIEKVQVLLVATRKEVTDTYV
ETFAQAGLKVTVLEVSSFSLIRTLKHQLEQFSSTEAAIIADIQFDSTELAIVVNGIPQFNRAIPIGLFQVQSALNSAMNL
PPTRDISELQAMTLPVTDTMGALNDPNPGTNAIIKVFAELADELRRSVDFYINQSDGLEMAQLLLTGPGAAIGQLDEFFM
QRLAMPATQVDPIESLALAFEREIPPEQRASLGVALGLGMRMT

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=1132917 NMG85_RS16945 WP_042154823.1 3686590..3687681(+) (pilM) [Planktothrix agardhii strain PCC7805]
ATGGTTAGTTTATTACAAGGATTGTTCTCCAAGCGGAAGCCAGGAGTCGGAATTGAATTAGCCACAGAGCGAATTAATCT
GGTGGAACTGCGGAAAAAAGGCAACCAATTAAAGTTAGTCACCTTAGCGACCACTCCGGTTCCCGAGGGCATTGTCCAAG
ATGGCCAAATTGTCGATACTCCAGCCATGGCGGAGTTAATTCAGTCCACTGTCACCGATAACAATATCAAAGCTAAGACC
GTTGGTACATCCATCCCCGGACGGGAAGCCGTAACCCGGATTATTCCGGTTCCCGCCGAACTCAATGACGACGAACTCAA
AGAATACATGAACGCCGAGTCGGGATTGTACCTCCCATTTCCCAGGGAAGAAGGAGACGTCGATTATCAAAAATTAGGGC
GATTTGTAGATGAGGATGGCATCGAAAAAGTACAGGTTTTATTAGTCGCCACCCGCAAAGAAGTGACGGATACCTATGTA
GAAACCTTTGCTCAAGCAGGTTTAAAAGTTACTGTATTAGAAGTTAGTAGTTTTTCTCTGATTCGTACCCTAAAACATCA
ACTTGAGCAGTTTTCCTCCACCGAAGCCGCGATTATTGCCGATATTCAATTTGATAGTACGGAATTGGCGATTGTTGTCA
ATGGCATTCCCCAGTTTAACCGCGCCATTCCTATTGGTCTGTTCCAAGTGCAAAGTGCCTTAAATAGTGCCATGAATTTA
CCTCCTACGAGGGATATCAGTGAATTACAGGCCATGACCCTTCCCGTGACTGATACAATGGGAGCATTAAACGATCCGAA
TCCCGGAACCAATGCCATCATTAAGGTTTTCGCCGAATTAGCCGATGAACTACGTCGCTCCGTTGACTTTTATATTAATC
AAAGTGATGGCTTAGAAATGGCACAGTTACTATTAACTGGCCCAGGGGCTGCTATTGGACAACTCGATGAGTTTTTCATG
CAACGGTTGGCCATGCCTGCCACCCAAGTTGACCCGATTGAATCCCTCGCCCTTGCCTTTGAGCGGGAAATTCCCCCAGA
ACAACGTGCTAGTTTAGGAGTGGCATTAGGTTTAGGAATGCGAATGACCTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-361)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J1JFB7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

54.521

100

0.548

Multiple sequence alignment