Detailed information NTG1132184

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: H1X04_RS05335
• Genome accession: NZ_LR822043
• Coordinates: 1013961..1014842 (-)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus isolate STH_CIRM_2101
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1008961..1019842

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1X04_RS05310 (STHERMO_1162)	vicR	1009661..1010368 (-)	708	WP_011227274.1	response regulator YycF	Regulator
H1X04_RS05315 (STHERMO_1164)	-	1010900..1011667 (+)	768	WP_011227275.1	amino acid ABC transporter ATP-binding protein	-
H1X04_RS05320 (STHERMO_1165)	-	1011679..1012512 (+)	834	WP_011227276.1	transporter substrate-binding domain-containing protein	-
H1X04_RS05325 (STHERMO_1166)	-	1012530..1013228 (+)	699	WP_011226087.1	amino acid ABC transporter permease	-
H1X04_RS05330 (STHERMO_1167)	-	1013240..1013890 (+)	651	WP_011226088.1	amino acid ABC transporter permease	-
H1X04_RS05335 (STHERMO_1168)	endA	1013961..1014842 (-)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
H1X04_RS05340 (STHERMO_1169)	-	1014904..1015092 (-)	189	WP_011226090.1	DNA-directed RNA polymerase subunit beta	-
H1X04_RS05345 (STHERMO_1170)	murA	1015094..1016365 (-)	1272	WP_041828250.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
H1X04_RS05350 (STHERMO_1171)	-	1016435..1016671 (-)	237	WP_179971627.1	DUF1146 family protein	-
H1X04_RS05355 (STHERMO_1172)	galE	1016859..1017872 (-)	1014	WP_011227279.1	UDP-glucose 4-epimerase GalE	-
H1X04_RS05360 (STHERMO_1173)	-	1017887..1018342 (-)	456	WP_041827047.1	DUF4832 domain-containing protein	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=1132184 H1X04_RS05335 WP_011226089.1 1013961..1014842(-) (endA) [Streptococcus thermophilus isolate STH_CIRM_2101]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=1132184 H1X04_RS05335 WP_011226089.1 1013961..1014842(-) (endA) [Streptococcus thermophilus isolate STH_CIRM_2101]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCCCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594