Detailed information NTG1132064

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: H1W94_RS05455
• Genome accession: NZ_LR822041
• Coordinates: 1026493..1027374 (-)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus isolate STH_CIRM_1122
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1021493..1032374

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1W94_RS05430 (STHERMO_1192)	vicR	1022193..1022900 (-)	708	WP_011227274.1	response regulator YycF	Regulator
H1W94_RS05435 (STHERMO_1194)	-	1023432..1024199 (+)	768	WP_011227275.1	amino acid ABC transporter ATP-binding protein	-
H1W94_RS05440 (STHERMO_1195)	-	1024211..1025044 (+)	834	WP_011227276.1	transporter substrate-binding domain-containing protein	-
H1W94_RS05445 (STHERMO_1196)	-	1025062..1025760 (+)	699	WP_011226087.1	amino acid ABC transporter permease	-
H1W94_RS05450 (STHERMO_1197)	-	1025772..1026422 (+)	651	WP_011226088.1	amino acid ABC transporter permease	-
H1W94_RS05455 (STHERMO_1198)	endA	1026493..1027374 (-)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
H1W94_RS05460 (STHERMO_1199)	-	1027436..1027624 (-)	189	WP_011227277.1	DNA-directed RNA polymerase subunit beta	-
H1W94_RS05465 (STHERMO_1200)	murA	1027626..1028897 (-)	1272	WP_041827046.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
H1W94_RS05470 (STHERMO_1201)	-	1028967..1029203 (-)	237	WP_002946959.1	DUF1146 family protein	-
H1W94_RS05475 (STHERMO_1202)	galE	1029391..1030404 (-)	1014	WP_011227279.1	UDP-glucose 4-epimerase GalE	-
H1W94_RS05480 (STHERMO_1203)	-	1030419..1030874 (-)	456	WP_041827047.1	DUF4832 domain-containing protein	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=1132064 H1W94_RS05455 WP_011226089.1 1026493..1027374(-) (endA) [Streptococcus thermophilus isolate STH_CIRM_1122]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=1132064 H1W94_RS05455 WP_011226089.1 1026493..1027374(-) (endA) [Streptococcus thermophilus isolate STH_CIRM_1122]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCCCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594