Detailed information NTG1130912

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: H0506_RS05335
• Genome accession: NZ_LR822015
• Coordinates: 1013289..1014170 (-)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus isolate STH_CIRM_65
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1008289..1019170

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0506_RS05310 (STHERMO_1174)	vicR	1008989..1009696 (-)	708	WP_011227274.1	response regulator YycF	Regulator
H0506_RS05315 (STHERMO_1176)	-	1010228..1010995 (+)	768	WP_011227275.1	amino acid ABC transporter ATP-binding protein	-
H0506_RS05320 (STHERMO_1177)	-	1011007..1011840 (+)	834	WP_011227276.1	transporter substrate-binding domain-containing protein	-
H0506_RS05325 (STHERMO_1178)	-	1011858..1012556 (+)	699	WP_011226087.1	amino acid ABC transporter permease	-
H0506_RS05330 (STHERMO_1179)	-	1012568..1013218 (+)	651	WP_011226088.1	amino acid ABC transporter permease	-
H0506_RS05335 (STHERMO_1180)	endA	1013289..1014170 (-)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
H0506_RS05340 (STHERMO_1181)	-	1014232..1014420 (-)	189	WP_011226090.1	DNA-directed RNA polymerase subunit beta	-
H0506_RS05345 (STHERMO_1182)	murA	1014422..1015693 (-)	1272	WP_041828250.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
H0506_RS05350 (STHERMO_1183)	-	1015763..1015999 (-)	237	WP_179971627.1	DUF1146 family protein	-
H0506_RS05355 (STHERMO_1184)	galE	1016187..1017200 (-)	1014	WP_011227279.1	UDP-glucose 4-epimerase GalE	-
H0506_RS05360 (STHERMO_1185)	-	1017215..1017670 (-)	456	WP_041827047.1	DUF4832 domain-containing protein	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=1130912 H0506_RS05335 WP_011226089.1 1013289..1014170(-) (endA) [Streptococcus thermophilus isolate STH_CIRM_65]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=1130912 H0506_RS05335 WP_011226089.1 1013289..1014170(-) (endA) [Streptococcus thermophilus isolate STH_CIRM_65]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCCCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594