Detailed information NTG1127280

Bioinformatically predicted

Overview

• Name: amiF
• Type: Regulator
• Locus tag: FGL04_RS01270
• Genome accession: NZ_LR594035
• Coordinates: 229761..230690 (+)
• Length: 309 a.a.
• NCBI ID: WP_138069139.1
• Uniprot ID: A0A4U9XLJ3
• Organism: Streptococcus pseudoporcinus strain NCTC5385
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 224761..235690

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FGL04_RS01250 (NCTC5385_00257)	-	225019..226677 (+)	1659	WP_138067933.1	peptide ABC transporter substrate-binding protein	-
FGL04_RS01255 (NCTC5385_00258)	-	226749..227663 (+)	915	WP_138067934.1	ABC transporter permease	-
FGL04_RS01260 (NCTC5385_00259)	-	227673..228704 (+)	1032	WP_138067935.1	ABC transporter permease	-
FGL04_RS01265	-	228715..229761 (+)	1047	Protein_213	ABC transporter ATP-binding protein	-
FGL04_RS01270 (NCTC5385_00262)	amiF	229761..230690 (+)	930	WP_138069139.1	ABC transporter ATP-binding protein	Regulator
FGL04_RS01275 (NCTC5385_00263)	rnjA	230742..232427 (-)	1686	WP_007892576.1	ribonuclease J1	-
FGL04_RS01280 (NCTC5385_00264)	-	232429..232659 (-)	231	WP_077322550.1	DNA-dependent RNA polymerase subunit epsilon	-
FGL04_RS01285 (NCTC5385_00265)	tsaB	232938..233630 (+)	693	WP_138067936.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
FGL04_RS01290 (NCTC5385_00266)	rimI	233630..234058 (+)	429	WP_138067937.1	ribosomal protein S18-alanine N-acetyltransferase	-
FGL04_RS01295 (NCTC5385_00267)	tsaD	234048..235058 (+)	1011	WP_138067938.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	-
FGL04_RS11295	-	235097..235213 (+)	117	Protein_220	branched-chain amino acid ABC transporter permease	-
FGL04_RS01300 (NCTC5385_00268)	rpsN	235417..235686 (-)	270	WP_003083690.1	30S ribosomal protein S14	-

Sequence

Protein

• Length: 309 a.a.
• Molecular weight: 35030.94 Da
• Isoelectric Point: 6.5104

>NTDB_id=1127280 FGL04_RS01270 WP_138069139.1 229761..230690(+) (amiF) [Streptococcus pseudoporcinus strain NCTC5385]
MTEEGKKLVELKNVSLIFNKGKKNEVKAIDNISFDIYEGEVFGLVGESGSGKTTVGRAILKLYDINEGDIIFNGKTISNL
KGKDLHEFRKDAQMIFQDPQASLNGRMKIRGIVAEGLDIHKLSPNKAEREERVQELLERVGLNRDHVTRYPHEFSGGQRQ
RIGIARALAVKPKFIIADEPISAPDVSIQAQVVNLMQRLQEERGLTYLFIAHDLSMVKYISDRIGVMHWGKMLEIGTSDD
VYNNPIHPYTRSLLSAIPEPDPDIERQRVAEIYDPSIEMDGQERHMHEITPGHFVLSTEAEAYRAQQRK

Nucleotide

• Length: 930 bp

>NTDB_id=1127280 FGL04_RS01270 WP_138069139.1 229761..230690(+) (amiF) [Streptococcus pseudoporcinus strain NCTC5385]
ATGACAGAAGAAGGTAAAAAATTAGTTGAGCTTAAGAATGTTTCACTGATCTTCAATAAGGGTAAAAAGAATGAAGTTAA
GGCTATTGACAATATCAGCTTTGATATCTATGAAGGTGAAGTCTTTGGTCTTGTCGGAGAATCTGGATCAGGAAAGACGA
CAGTTGGGCGTGCTATTTTGAAGCTTTATGATATCAATGAAGGTGATATTATCTTTAATGGTAAAACGATTTCAAATCTT
AAAGGTAAAGACTTACATGAGTTCCGTAAAGATGCCCAGATGATTTTCCAAGATCCACAAGCTAGTCTTAATGGGCGGAT
GAAAATTAGAGGCATTGTTGCAGAAGGTCTAGATATTCATAAACTTTCACCAAACAAAGCAGAGCGTGAAGAACGGGTCC
AGGAATTACTTGAACGTGTTGGCTTGAACCGCGACCATGTCACACGTTACCCTCATGAATTTTCTGGCGGTCAACGTCAA
CGGATTGGTATTGCAAGAGCTTTGGCTGTTAAACCTAAGTTCATCATTGCTGACGAGCCAATATCTGCTCCAGATGTCTC
CATTCAAGCACAGGTTGTTAACTTAATGCAACGATTACAAGAAGAAAGAGGTTTGACTTATCTCTTTATTGCTCATGATT
TGTCAATGGTTAAATATATTTCCGATCGTATTGGTGTTATGCATTGGGGCAAGATGTTAGAGATTGGAACTTCTGATGAT
GTTTACAATAACCCTATTCATCCCTACACTAGGAGCCTCCTCTCTGCTATCCCAGAACCAGATCCTGATATTGAGCGTCA
ACGGGTTGCTGAAATTTACGATCCAAGTATCGAAATGGACGGACAAGAACGTCATATGCATGAAATTACGCCAGGTCACT
TTGTTTTATCGACCGAAGCAGAAGCATATAGAGCTCAACAAAGAAAGTAA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (30-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-265)

3D structure

• AlphaFold DB: A0A4U9XLJ3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiF	Streptococcus thermophilus LMD-9	53.871	100	0.54
amiF	Streptococcus thermophilus LMG 18311	53.548	100	0.537
amiF	Streptococcus salivarius strain HSISS4	52.581	100	0.528