Detailed information NTG1126137

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: E0F32_RS09460
• Genome accession: NZ_LR216050
• Coordinates: 1756561..1757385 (-)
• Length: 274 a.a.
• NCBI ID: WP_001036786.1
• Uniprot ID: A0A0B7LDF1
• Organism: Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1751561..1762385

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F32_RS09455 (SAMEA3431333_01776)	-	1754939..1756270 (-)	1332	WP_016398203.1	hemolysin family protein	-
E0F32_RS09460 (SAMEA3431333_01777)	endA	1756561..1757385 (-)	825	WP_001036786.1	DNA-entry nuclease EndA	Machinery gene
E0F32_RS09465 (SAMEA3431333_01778)	-	1757424..1757612 (-)	189	WP_001813390.1	DNA-directed RNA polymerase subunit beta	-
E0F32_RS09470 (SAMEA3431333_01779)	murA	1757605..1758888 (-)	1284	WP_000358028.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
E0F32_RS09475 (SAMEA3431333_01780)	-	1758912..1759142 (-)	231	WP_000250392.1	DUF1146 family protein	-
E0F32_RS09480 (SAMEA3431333_01781)	sepM	1759200..1760237 (-)	1038	WP_000727276.1	SepM family pheromone-processing serine protease	Regulator
E0F32_RS09485 (SAMEA3431333_01782)	coaD	1760221..1760709 (-)	489	WP_001280742.1	pantetheine-phosphate adenylyltransferase	-
E0F32_RS09490 (SAMEA3431333_01783)	rsmD	1760699..1761238 (-)	540	WP_000706962.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
E0F32_RS09495 (SAMEA3431333_01784)	asnA	1761303..1762295 (-)	993	WP_000747993.1	aspartate--ammonia ligase	-

Sequence

Protein

• Length: 274 a.a.
• Molecular weight: 29921.59 Da
• Isoelectric Point: 10.1197

>NTDB_id=1126137 E0F32_RS09460 WP_001036786.1 1756561..1757385(-) (endA) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide

• Length: 825 bp

>NTDB_id=1126137 E0F32_RS09460 WP_001036786.1 1756561..1757385(-) (endA) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (75-247)

3D structure

• AlphaFold DB: A0A0B7LDF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	99.635	100	0.996
endA	Streptococcus pneumoniae D39	99.635	100	0.996
endA	Streptococcus pneumoniae R6	99.635	100	0.996
endA	Streptococcus pneumoniae TIGR4	99.635	100	0.996