Detailed information NTG1122038

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: EL147_RS09900
• Genome accession: NZ_LR134341
• Coordinates: 2017185..2018054 (+)
• Length: 289 a.a.
• NCBI ID: WP_007894365.1
• Uniprot ID: G5K6Q8
• Organism: Streptococcus pseudoporcinus strain NCTC13786
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 2012185..2023054

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL147_RS09870 (NCTC13786_01986)	-	2012480..2013358 (+)	879	WP_007894114.1	F0F1 ATP synthase subunit gamma	-
EL147_RS09875 (NCTC13786_01987)	atpD	2013476..2014882 (+)	1407	WP_007894308.1	F0F1 ATP synthase subunit beta	-
EL147_RS09880 (NCTC13786_01988)	-	2014895..2015311 (+)	417	WP_007894322.1	F0F1 ATP synthase subunit epsilon	-
EL147_RS09885 (NCTC13786_01989)	-	2015393..2015617 (+)	225	WP_037598858.1	DUF1146 family protein	-
EL147_RS09890 (NCTC13786_01990)	murA	2015692..2016963 (+)	1272	WP_007894157.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
EL147_RS09895 (NCTC13786_01991)	-	2016963..2017154 (+)	192	WP_176602246.1	DNA-directed RNA polymerase subunit beta	-
EL147_RS09900 (NCTC13786_01992)	endA	2017185..2018054 (+)	870	WP_007894365.1	DNA/RNA non-specific endonuclease	Machinery gene
EL147_RS09905 (NCTC13786_01993)	pheS	2018359..2019402 (+)	1044	WP_007894315.1	phenylalanine--tRNA ligase subunit alpha	-
EL147_RS09910 (NCTC13786_01994)	-	2019447..2019971 (+)	525	WP_007894116.1	N-acetyltransferase	-
EL147_RS09915 (NCTC13786_01995)	pheT	2019986..2022391 (+)	2406	WP_007895982.1	phenylalanine--tRNA ligase subunit beta	-
EL147_RS09920 (NCTC13786_01996)	-	2022483..2022857 (+)	375	WP_007894293.1	OsmC family protein	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32097.31 Da
• Isoelectric Point: 10.4558

>NTDB_id=1122038 EL147_RS09900 WP_007894365.1 2017185..2018054(+) (endA) [Streptococcus pseudoporcinus strain NCTC13786]
MSKKQLNIQKLNGILLALLITGVIALPQSRHLASDNPFQQVLQIISGQGKTQKYSKNPTNPDTPSYQLASSVLTQQVKKQ
LGQKIEWNGAGAFIINNNRTDLKVKVSSLPYANNDIKRVEGKQVPFRANALLAKSTRQYRNRNETGNGYTSWKPAGWHQI
HGLSGGYDHAIDRGHLLAYALIGGLKDYDASTSNPSNIAVQTAWANQAYLSDSTGQNYYETLIRKALDSGKRVRYRVTLL
YEKNNLVSSGSHLEAKANDGSLEFNVFVPNVQKGLKINYQTGQVKRHMN

Nucleotide

• Length: 870 bp

>NTDB_id=1122038 EL147_RS09900 WP_007894365.1 2017185..2018054(+) (endA) [Streptococcus pseudoporcinus strain NCTC13786]
ATGTCCAAAAAACAGCTAAATATCCAAAAGCTCAATGGGATTCTGCTAGCTCTGCTCATTACTGGGGTAATAGCCTTACC
GCAGAGCCGTCATCTTGCGTCTGATAACCCATTTCAGCAAGTATTACAGATTATTTCCGGTCAAGGGAAAACTCAAAAAT
ACTCCAAAAATCCGACGAATCCTGATACACCTAGTTATCAGTTAGCTAGTTCAGTGCTTACGCAACAAGTGAAAAAACAA
TTAGGTCAGAAGATTGAGTGGAATGGTGCTGGTGCTTTTATCATTAACAATAATAGGACCGATTTGAAGGTAAAAGTTTC
AAGTTTACCTTATGCCAATAATGACATCAAGCGAGTTGAGGGTAAGCAAGTGCCTTTCAGAGCGAATGCTTTATTAGCTA
AATCAACAAGACAATACCGTAACCGCAATGAAACGGGGAATGGCTATACCTCTTGGAAGCCAGCGGGGTGGCATCAAATT
CATGGCTTGTCAGGTGGATATGATCATGCTATTGATAGGGGGCATTTACTAGCTTATGCATTGATAGGTGGCCTAAAAGA
TTATGACGCTTCTACCAGCAATCCAAGTAACATTGCAGTACAAACGGCCTGGGCAAACCAAGCTTACCTCTCAGATTCAA
CAGGTCAAAATTATTATGAAACCTTAATACGAAAAGCACTAGATAGCGGTAAACGGGTTCGTTACAGAGTGACCTTGCTT
TATGAGAAGAATAACCTTGTTTCTAGCGGTAGTCACTTAGAAGCAAAAGCTAATGATGGCAGTTTGGAATTTAATGTTTT
TGTGCCAAATGTTCAAAAAGGTTTAAAGATTAACTATCAAACAGGACAAGTTAAGCGACACATGAATTAA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (94-246)

3D structure

• AlphaFold DB: G5K6Q8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	65.625	77.509	0.509
endA	Streptococcus pneumoniae D39	65.625	77.509	0.509
endA	Streptococcus pneumoniae R6	65.625	77.509	0.509
endA	Streptococcus pneumoniae TIGR4	65.625	77.509	0.509