Detailed information NTG1122019

Bioinformatically predicted

Overview

• Name: amiF
• Type: Regulator
• Locus tag: EL147_RS07140
• Genome accession: NZ_LR134341
• Coordinates: 1460835..1461770 (+)
• Length: 311 a.a.
• NCBI ID: WP_007892581.1
• Uniprot ID: G5K8C0
• Organism: Streptococcus pseudoporcinus strain NCTC13786
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 1455835..1466770

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL147_RS07120 (NCTC13786_01429)	-	1456093..1457751 (+)	1659	WP_007892405.1	peptide ABC transporter substrate-binding protein	-
EL147_RS07125 (NCTC13786_01430)	-	1457823..1458737 (+)	915	WP_007896266.1	ABC transporter permease	-
EL147_RS07130 (NCTC13786_01431)	-	1458747..1459778 (+)	1032	WP_007892413.1	ABC transporter permease	-
EL147_RS07135 (NCTC13786_01432)	oppD	1459789..1460835 (+)	1047	WP_007892284.1	ABC transporter ATP-binding protein	Regulator
EL147_RS07140 (NCTC13786_01433)	amiF	1460835..1461770 (+)	936	WP_007892581.1	ABC transporter ATP-binding protein	Regulator
EL147_RS07145 (NCTC13786_01434)	rnjA	1461822..1463507 (-)	1686	WP_007892576.1	ribonuclease J1	-
EL147_RS07150 (NCTC13786_01435)	-	1463509..1463739 (-)	231	WP_007892277.1	DNA-dependent RNA polymerase subunit epsilon	-
EL147_RS07155 (NCTC13786_01436)	tsaB	1464018..1464710 (+)	693	WP_007892554.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
EL147_RS07160 (NCTC13786_01437)	rimI	1464710..1465138 (+)	429	WP_007892326.1	ribosomal protein S18-alanine N-acetyltransferase	-
EL147_RS07165 (NCTC13786_01438)	tsaD	1465128..1466138 (+)	1011	WP_007892250.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	-
EL147_RS07170 (NCTC13786_01439)	rpsN	1466497..1466766 (-)	270	WP_003083690.1	30S ribosomal protein S14	-

Sequence

Protein

• Length: 311 a.a.
• Molecular weight: 35305.22 Da
• Isoelectric Point: 6.1904

>NTDB_id=1122019 EL147_RS07140 WP_007892581.1 1460835..1461770(+) (amiF) [Streptococcus pseudoporcinus strain NCTC13786]
MTEEGKKLVELKNVSLIFNKGKKNEVKAIDNISFDIYEGEVFGLVGESGSGKTTVGRAILKLYDINEGDIIFNGKTISNL
KGKDLHEFRKDAQMIFQDPQASLNGRMKIRDIVAEGLDIHKLSPNKAEREERVQELLERVGLNRDHVTRYPHEFSGGQRQ
RIGIARALAVKPKFIIADEPISALDVSIQAQVVNLMQRLQEERGLTYLFIAHDLSMVKYISDRIGVMHWGKMLEIGTSDD
VYNNPIHPYTRSLLSAIPEPDPDIERQRVAEIYDPSIEMDGQERHMHEITPGHFVLSTEAEAEAYRAQQRK

Nucleotide

• Length: 936 bp

>NTDB_id=1122019 EL147_RS07140 WP_007892581.1 1460835..1461770(+) (amiF) [Streptococcus pseudoporcinus strain NCTC13786]
ATGACAGAAGAAGGTAAAAAATTAGTTGAGCTTAAGAATGTTTCACTGATCTTCAATAAGGGTAAAAAGAATGAAGTTAA
GGCTATTGACAATATCAGCTTTGATATCTATGAAGGTGAAGTCTTTGGTCTTGTCGGAGAATCTGGATCAGGAAAGACGA
CAGTTGGCCGTGCTATTTTGAAGCTTTATGATATCAATGAAGGTGATATTATCTTTAATGGCAAAACGATTTCAAATCTT
AAAGGTAAAGACTTACATGAGTTCCGTAAAGATGCCCAGATGATTTTCCAAGATCCACAAGCTAGTCTTAATGGGCGGAT
GAAAATTAGAGACATTGTTGCAGAAGGTCTAGATATTCATAAACTTTCACCAAATAAAGCAGAGCGTGAAGAACGGGTCC
AGGAATTACTTGAACGTGTTGGCTTGAACCGCGACCATGTCACACGTTACCCTCATGAATTTTCTGGCGGTCAACGTCAA
CGGATTGGTATTGCAAGAGCTTTGGCTGTTAAACCTAAGTTCATCATTGCTGACGAGCCAATATCTGCTCTAGACGTCTC
CATTCAAGCACAGGTTGTTAACTTAATGCAACGATTACAAGAAGAAAGAGGTTTGACTTATCTCTTTATTGCTCATGATT
TGTCAATGGTTAAATATATTTCCGATCGTATTGGTGTTATGCATTGGGGCAAGATGTTAGAGATTGGAACTTCTGATGAT
GTTTACAATAACCCTATTCATCCCTACACTAGGAGCCTCCTCTCTGCTATCCCAGAACCAGATCCTGATATTGAGCGTCA
ACGGGTTGCTGAAATTTACGATCCAAGTATCGAAATGGACGGACAAGAACGTCATATGCATGAAATTACGCCAGGTCACT
TTGTTTTATCGACCGAAGCAGAAGCAGAAGCATATAGAGCTCAACAAAGAAAGTAA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (30-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-265)

3D structure

• AlphaFold DB: G5K8C0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiF	Streptococcus thermophilus LMD-9	53.871	99.678	0.537
amiF	Streptococcus thermophilus LMG 18311	53.548	99.678	0.534
amiF	Streptococcus salivarius strain HSISS4	53.226	99.678	0.531