Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   EW023_RS09700 Genome accession   NZ_LR130239
Coordinates   594814..595680 (+) Length   288 a.a.
NCBI ID   WP_023612376.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain PS003 isolate PS003     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 589814..600680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW023_RS03090 - 589855..590730 (+) 876 WP_002985236.1 F0F1 ATP synthase subunit gamma -
  EW023_RS03095 atpD 590892..592298 (+) 1407 WP_002985235.1 F0F1 ATP synthase subunit beta -
  EW023_RS03100 - 592311..592727 (+) 417 WP_002985233.1 F0F1 ATP synthase subunit epsilon -
  EW023_RS03105 - 593026..593250 (+) 225 WP_002985230.1 DUF1146 family protein -
  EW023_RS03110 murA 593315..594586 (+) 1272 WP_002990414.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  EW023_RS03115 - 594590..594778 (+) 189 WP_011017613.1 DNA-directed RNA polymerase subunit beta -
  EW023_RS09700 endA 594814..595680 (+) 867 WP_023612376.1 DNA/RNA non-specific endonuclease Machinery gene
  EW023_RS03125 pheS 595963..597006 (+) 1044 WP_003057440.1 phenylalanine--tRNA ligase subunit alpha -
  EW023_RS03130 pheT 597216..599621 (+) 2406 WP_032465590.1 phenylalanine--tRNA ligase subunit beta -
  EW023_RS03135 - 599731..600108 (+) 378 WP_002993028.1 OsmC family protein -
  EW023_RS03140 - 600101..600487 (+) 387 WP_002990406.1 YbgA family protein -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 32087.59 Da        Isoelectric Point: 10.5080

>NTDB_id=1116703 EW023_RS09700 WP_023612376.1 594814..595680(+) (endA) [Streptococcus pyogenes strain PS003 isolate PS003]
MLAKKKRKRVLNSFLLILLVLGSNCLAKSDFIASNHPIKQLYQTLVGEKKKSLLTHNNLPADVPTEALAASVLTPSIKKQ
LGPDVQWNGAGAYIINRNKTDLNAKVASLPYANNETKWVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRNFKPAGWHRL
HHLKGSYDHAVDRGHLLGYALVGGLKGLDASTGNPDNIATQLSWANQANKPYSTGQNYYEGLVRRALDKGHRVRYRVTLL
YDGDNLLASGSHLEAKSSDGSLTFNVFVPNVQPGLTVDYRTRQIAINL

Nucleotide


Download         Length: 867 bp        

>NTDB_id=1116703 EW023_RS09700 WP_023612376.1 594814..595680(+) (endA) [Streptococcus pyogenes strain PS003 isolate PS003]
ATGTTAGCAAAGAAAAAACGAAAAAGGGTGCTTAATTCCTTCTTATTGATTTTGTTAGTGTTGGGGTCTAACTGCCTTGC
TAAGAGTGATTTCATAGCTAGCAATCACCCTATCAAGCAACTTTATCAGACTCTTGTTGGGGAGAAGAAAAAATCATTAC
TCACACATAATAATTTGCCCGCTGATGTTCCCACAGAAGCTCTGGCGGCATCGGTATTAACACCATCAATCAAAAAGCAA
CTAGGTCCAGATGTTCAGTGGAATGGAGCAGGCGCCTATATTATCAACAGGAACAAAACAGATTTAAATGCTAAGGTAGC
CAGTCTGCCCTATGCCAACAATGAAACCAAATGGGTGCAAGAGCAAAAGGTTCCTAAAAGAGCTAATGCCTTACTTACTA
AGTCGACACGACAGTATCGAAATCGCTATCAAACGGACAATGCTTATCGTAACTTTAAGCCAGCAGGTTGGCACCGATTG
CACCACTTAAAAGGAAGTTATGATCACGCGGTTGATAGAGGGCATTTGCTAGGTTATGCTTTAGTTGGTGGACTGAAAGG
TTTAGATGCTTCTACCGGTAATCCTGATAATATTGCCACTCAACTAAGTTGGGCTAATCAAGCAAATAAACCCTACTCGA
CAGGTCAGAATTACTATGAAGGTTTAGTACGTCGTGCTTTAGATAAGGGGCATCGCGTCCGCTACCGTGTCACCCTACTT
TATGATGGTGATAATCTACTAGCCAGTGGTAGCCATTTGGAGGCTAAATCCTCTGATGGCAGCTTGACTTTTAACGTGTT
TGTTCCAAATGTCCAACCAGGATTGACCGTTGATTATAGGACGAGACAAATAGCTATCAATCTCTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(111-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

58.65

82.292

0.483
  endA Streptococcus pneumoniae D39

58.65

82.292

0.483
  endA Streptococcus pneumoniae R6

58.65

82.292

0.483
  endA Streptococcus pneumoniae TIGR4

58.65

82.292

0.483

Multiple sequence alignment