Detailed information    

experimental Experimentally validated

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NGFG_RS00510 Genome accession   NC_022240
Coordinates   101039..103233 (-) Length   730 a.a.
NCBI ID   Protein_92    Uniprot ID   -
Organism   Neisseria gonorrhoeae MS11     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilQ is proposed to function in the terminal steps of organelle biogenesis by acting as a pilus channel or pore. pilQ mutants are severely defective for both pilus expression and competence for natural transformation.


Genomic Context

Location: 96039..108233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS00490 (NGFG_00226) - 96399..96863 (-) 465 WP_003687344.1 transcriptional regulator NrdR -
  NGFG_RS00495 (NGFG_00227) - 96892..97734 (-) 843 WP_003687345.1 class II glutamine amidotransferase -
  NGFG_RS00500 (NGFG_00230) aroB 98473..99552 (-) 1080 WP_003687348.1 3-dehydroquinate synthase -
  NGFG_RS00505 (NGFG_00231) - 99632..100144 (-) 513 WP_003687349.1 shikimate kinase -
  NGFG_RS00510 (NGFG_00232) pilQ 101039..103233 (-) 2195 Protein_92 type IV pilus secretin PilQ Machinery gene
  NGFG_RS00515 (NGFG_00233) pilP 103252..103797 (-) 546 WP_003687351.1 pilus assembly protein PilP Machinery gene
  NGFG_RS00520 (NGFG_00234) - 103815..104462 (-) 648 WP_003694754.1 type 4a pilus biogenesis protein PilO -
  NGFG_RS00525 (NGFG_00235) pilN 104463..105062 (-) 600 WP_003687354.1 PilN domain-containing protein Machinery gene
  NGFG_RS00530 (NGFG_00236) pilM 105065..106180 (-) 1116 WP_003687356.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 730 a.a.        Molecular weight: Da        Isoelectric Point:

>NTDB_id=1114 NGFG_RS00510 Protein_92 101039..103233(-) (pilQ) [Neisseria gonorrhoeae MS11]
None

Nucleotide


Download         Length: 2195 bp        

>NTDB_id=1114 NGFG_RS00510 Protein_92 101039..103233(-) (pilQ) [Neisseria gonorrhoeae MS11]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACGGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATTTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCCCGCCCAGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTA
TCCGTATCCGCACCGTTCAGCCCGGCAAAACAACAGGCAGCGGCATCGGCAAAACAACAGGCGGCGACACCGGCAAAACA
AACCAATATCGATTTCCGCAAAGACGGCAAAAATGCCGGCATTATCGAATTGGCGGCATTGGGCTTTGCCGGGCAGCCCG
ACATCAGCCAACAGCACGACCACATCATCGTTACGCTGAAAAACCATACCCTGCCGACCGCGCTCCAACGCAGTTTGGAT
GTGGCAGACTTCAAAACACCGGTTCAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTATCACAACAAC
CGGCAACTGGGAACTCGTCAACAAATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCG
AGTCAGGCGGCGTGAACAATGCGCCCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGTCGAAATCCGC
ACCATCCTGCAGATTTTGGCAAAAGAATCCGGGATGAACATTGTTGCCAGCGACTCCGTCAACGGCAAAATGACCCTCTC
CCTCAAAGATGTGCCTTGGGATCAGGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAGCAAGGGAACA
TCGTCAACATCGCGCCCCGCGACGAGCTGCTTGCCAAAGACAAAGCCTTCTTACAAGCGGAAAAAGACATTGCCGATCTG
GGCGCGCTGTATTCCCAAAACTTCCAATTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTTTGGACAATGC
CGACACGACCGGAAACCGCAACACGCTTGTCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAACACCCTGATTG
TTACCGATACCCGCAGCGTCATCGAAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACAAGTGATGATT
GAGGCGCGTATCGTCGAAGCGGCAGACGGCTTCTCGCGCGATTTGGGCGTTAAGTTCGGCGCGACAGGCAGGAAAAAACT
GAAAAATGAGACGAGCGCATTCGGCTGGGGCGTGAACTCCGGCTTCGGGGGCGGCGATAAATGGGAGGCCCAAACCAAAA
TCAACCTGCCGGTTGCCGCTGCCGCAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGCGCGTTGAATTTGGAATTG
TCCGCATCCGAGTCGCTTTCAAAAACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAGAGGCCAAAAT
CGAATCCGGTTACGAAATTCCTTTTACCGTAACTACAGCCTCGGGCGGCGGCAACTCTACCAACACGGAACTCAAAAAAG
CCGTCTTGGGGCTGACCGTTACGCCGAACATCACGCCCGACGGACAAATCATCATGACCGTCAAAATCAACAAAGACTCG
CCTGCACAATGTGCTTCAGGCAGCAACACAATCCTATGTATTTCGACCAAAAGCCTGAATACGCAGGCTATGGTTGAAAA
CGGCGGCACTTTGATTGTCGGCGGTATTTATGAAGAAAACAACGGCAATACGCTGACCAAAGTCCCCCTGTTGGGCGACA
TCCCCGTTATCGGCAACCTCTTTAAAACACGCGGGAAAAAACCGACCGCCGCGAACTGCTGATTTTCATTACCCCGAGGA
TTATAGATACGGCCGGCAACAGCCTGCGCTATTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] S L Drake et al. (1995) The product of the pilQ gene is essential for the biogenesis of type IV pili in Neisseria gonorrhoeae. Molecular Microbiology 18(5):975-86. [PMID: 8825101]