Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ACNBEW_RS05370 Genome accession   NZ_CP184280
Coordinates   1038139..1041639 (+) Length   1166 a.a.
NCBI ID   WP_032874392.1    Uniprot ID   -
Organism   Bacillus velezensis strain GHZJ     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1033139..1046639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNBEW_RS05345 (ACNBEW_05345) - 1033321..1034478 (-) 1158 WP_014304750.1 AbrB family transcriptional regulator -
  ACNBEW_RS05350 (ACNBEW_05350) - 1034646..1035842 (-) 1197 WP_003155154.1 MFS transporter -
  ACNBEW_RS05355 (ACNBEW_05355) - 1035858..1036478 (-) 621 WP_003155153.1 TetR/AcrR family transcriptional regulator -
  ACNBEW_RS05360 (ACNBEW_05360) - 1036707..1037216 (-) 510 WP_014304752.1 ferritin-like domain-containing protein -
  ACNBEW_RS05365 (ACNBEW_05365) - 1037285..1037998 (-) 714 WP_079891127.1 DUF421 domain-containing protein -
  ACNBEW_RS05370 (ACNBEW_05370) addB 1038139..1041639 (+) 3501 WP_032874392.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACNBEW_RS05375 (ACNBEW_05375) addA 1041626..1045330 (+) 3705 WP_032875181.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACNBEW_RS05380 (ACNBEW_05380) sbcD 1045395..1046567 (+) 1173 WP_032874394.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133367.85 Da        Isoelectric Point: 5.3149

>NTDB_id=1108157 ACNBEW_RS05370 WP_032874392.1 1038139..1041639(+) (addB) [Bacillus velezensis strain GHZJ]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEYKHEFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHAPELACIESQYDVRPAAAYTGGQEAFTVMQAQNR
RAELEGIAREIQSLVRDGGYRYKDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTSGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
IAAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQRAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEI
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKESDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLDAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPKLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPVGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDRGLDLAEVYYGLALQMLTYLDLSITHSEDWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIVNAGLKKDGSLRSDSAAVSEQDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=1108157 ACNBEW_RS05370 WP_032874392.1 1038139..1041639(+) (addB) [Bacillus velezensis strain GHZJ]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGG
GGCATGAACCGCCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAATATAAGCACGAGTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGATTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGACACTATTTGCATTCAGAGGACTATTT
GACGCTGCTCGCTCAGCAGATTCCTTTGGCGGACGTTGTAAAAGGCGCCCGTGTTTACGTCGACGGATTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCACCTGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCTGATATTTCCTGCAAGGAACTGCATGGCACGAAGCGGCATCAGCATGCACCGGAGCTTGCCTGTATAG
AGTCACAGTACGATGTGCGTCCCGCCGCGGCGTATACCGGCGGACAGGAAGCTTTTACCGTCATGCAGGCGCAAAACAGA
AGAGCTGAGCTTGAAGGTATTGCCCGGGAAATTCAATCGCTTGTCCGGGACGGAGGATACCGGTACAAAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCCGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGTTCAAGCCTCGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCAGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTTGA
TCAGCTTGAAAATTACTGTATTGCCTACGGCATTAAAGGGGAGCGCTGGACAAGCGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTTGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAGAGAGTGG
ATAGCCGCGCCCCTCGTCCGGCTTCAAAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAGGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCACTGAAGCTGGACCGGAAAAGGCAGCGGGCCGAAGAGGCCGGAAACATGATCGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATA
TCATTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCGCCTGCTCTTGACCA
AGTATTTGTAGGGAATATGGATCTCTCAAGAATGTACGGCACGTCCTGCACATTCGTCATCGGGGCTAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAGAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCGGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCGAGTCCGTCTGACCGTCTTTATGT
GTCATACCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCTCAGACATATACGGCAAGTCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGTCTGATCACCGGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCATATTAAAGGAAGCGTCTCACGGATGGAGGCGTTTAATGCC
TGCCAGTTCTCTCATTTTGCATCTCACGGGCTTCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGACGCGCCGGATATCGG
CCAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGAGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAAGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTTTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGGTCGGTCTTGAACTCGGTTTCGGCGGAAGCGGGCCGCTGCCCCCGCTCACTTTCACGCTGAAAAACG
GCTGTACAATGGAACTCGTCGGACGGATCGACCGCGTGGATAAAGCGGAAAGCTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGACAGAGGGCTTGATCTGGCTGAGGTGTATTACGGGCTCGCTCTGCAAATGCTCACATACCTTGA
CTTATCGATTACACATTCCGAAGATTGGCTCGGTATGAAAGCGACACCGGCAGGCGTTTTGTATTTCCATATCCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTTAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGTCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATCGTCAATGCCGGTTT
GAAAAAGGACGGGTCTTTAAGATCCGACTCAGCCGCGGTCAGCGAACAGGACTTTCATCTGCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCTGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAGTCGGTATGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.448

100

0.834

Multiple sequence alignment