Detailed information    

experimental Experimentally validated

Overview

Name   dca   Type   Unclear
Locus tag   VT05_RS06875 Genome accession   NZ_CP012026
Coordinates   1298252..1299898 (-) Length   548 a.a.
NCBI ID   WP_003693549.1    Uniprot ID   A0AA44ZFS4
Organism   Neisseria gonorrhoeae strain FA19     
Function   require for natural transformation   
Unclear

Function

A deletion and insertional mutation was made in the dca gene of N. gonorrhoeae strain FA19 and N. meningitidis strain NMB. This mutation abrogated the ability of the gonococci to be transformed with chromosomal DNA.


Genomic Context

Location: 1293252..1304898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VT05_RS06850 (VT05_01311) murD 1293336..1294673 (-) 1338 WP_003689446.1 UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase -
  VT05_RS06855 (VT05_01312) - 1294758..1295138 (-) 381 WP_003689448.1 tetratricopeptide repeat protein -
  VT05_RS06860 (VT05_01313) mraY 1295263..1296345 (-) 1083 WP_003689450.1 phospho-N-acetylmuramoyl-pentapeptide- transferase -
  VT05_RS15315 (VT05_01314) - 1296590..1296766 (-) 177 WP_003689452.1 hypothetical protein -
  VT05_RS06870 (VT05_01315) murF 1296769..1298127 (-) 1359 WP_017147009.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
  VT05_RS06875 (VT05_01316) dca 1298252..1299898 (-) 1647 WP_003693549.1 phosphoethanolamine transferase Unclear
  VT05_RS06880 (VT05_01317) - 1299921..1301399 (-) 1479 WP_003693548.1 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase -
  VT05_RS06885 (VT05_01318) - 1301424..1303169 (-) 1746 WP_003693546.1 penicillin-binding protein 2 -
  VT05_RS06890 (VT05_01319) ftsL 1303230..1303493 (-) 264 WP_003689461.1 cell division protein FtsL -
  VT05_RS06895 (VT05_01320) rsmH 1303520..1304476 (-) 957 WP_003689462.1 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH -

Sequence

Protein


Download         Length: 548 a.a.        Molecular weight: 61162.07 Da        Isoelectric Point: 9.4421

>NTDB_id=1107 VT05_RS06875 WP_003693549.1 1298252..1299898(-) (dca) [Neisseria gonorrhoeae strain FA19]
MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGYRYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTT
ALYLPVGWLYGAPSYQIVGSILESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKKASTWHIRHVAPKYKNYVVVIGE
SARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAHATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFA
NEISTYALRSDYPWFTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARRFQYQTEKI
SCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLTHGEWKRQSYGVPLVKISSDDTRREMIKVRR
SAFNFLRGFGSWTGIETDELPDDGYDFWGNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK

Nucleotide


Download         Length: 1647 bp        

>NTDB_id=1107 VT05_RS06875 WP_003693549.1 1298252..1299898(-) (dca) [Neisseria gonorrhoeae strain FA19]
ATGAAACAATCCGCCCGAATAAAAAATATGGATCAGACATTAAAAAATACATTGGGCATTTGCGCGCTTTTAGCCTTTTG
TTTTGGCGCGGCCATCGCATCAGGTTATCACTTGGAATATGAATACGGCTACCGTTATTCTGCCGTGGGCGCTTTGGCTT
CGGTTGTATTTTTATTATTATTGGCACGCGGCTTCCCGCGCGTTTCTTCAGTTGTTTTACTGATTTACGTCGGCACAACC
GCCCTATATTTGCCGGTCGGCTGGCTGTATGGTGCGCCTTCTTATCAGATAGTCGGTTCGATATTGGAAAGCAATCCTGC
CGAGGCGCGTGAATTTGTCGGCAATCTTCCCGGGTCGCTTTATTTTGTGCAGGCATTATTTTTCATTTTTGGCTTGACAG
TTTGGAAATATTGTGTATCTGTGGGGGTATTTGCTGACGTAAAAAACTATAAACGCCGCAGCAAAATATGGCTGACCATA
TTATTGACTTTGATTTTGTCCTGCGCGGTGATGGAGAAAATCGCCGGCGATAAAGATTGGCGAGAACCTGATGCCGGCCT
GTTGTTGAATATTTTCGACCTGTATTACGATTTGGCTTCCGCGCCGGCACAATATGCCGCCAAGCGCGCCCACATTTTGG
AAGCAGCAAAAAAAGCGTCAACATGGCATATCCGTCATGTTGCGCCCAAGTATAAAAATTATGTTGTGGTTATCGGTGAG
AGCGCGCGTTCGGATTATATGAATGTTTACGGTTTCCCATTGCCCGATACGCCTTTTTTGAGTCGGACCAAAGGGCTGTT
GATAAACGGTTACCAATCGACCGCCCACGCGACGAATCTTTCGCTGCCGCAGACTTTGGGGCTGCCGGGAGAGCCGAACA
ATAACATTGTCAGCTTGGCGAAGCAGGCGGGTTTTCGGACGGCGTGGCTGTCTAATCAAGGAATGTTGGGGCATTTTGCC
AACGAAATTTCCACCTATGCCCTACGCAGCGATTATCCGTGGTTTACCCAAAGGGGTGATTATGGCAAAAGCGCGGGGTT
GAGCGACCGCCTTTTGTTGCCGGCGTTCAAACGGGTTTTGACAGGAAATGCAGGCACGAAGCCTCGGCTGATTGTGATGC
ACCTGATGGGTTCGCACAGTGATTTTTGCACACGTTTGGATAAGGATGCGCGGCGGTTTCAGTATCAAACTGAAAAAATA
TCCTGCTATGTTTCCACCATCGCGCAAACCGATAAATTTTTAGAAGATACGGTTAAGATATTGAATGAAAATAAAGAAAG
CTGGTCTTTGGTTTACTTTTCCGACCACGGTTTGATGCATGTCGGCAAAGGCGGCGAGCGAACGTTGACACACGGCGAGT
GGAAGCGTCAAAGCTACGGCGTGCCGCTGGTTAAAATTTCGTCCGATGACACGCGGCGCGAAATGATTAAAGTGAGGCGC
AGCGCGTTTAATTTTTTACGCGGATTCGGCAGTTGGACGGGTATCGAAACCGACGAGTTGCCCGATGACGGCTATGATTT
TTGGGGGAATGTCCCCGATGTGCCGGGCGAAGGCAATAACCTTGCCTTTATCGACAGACAGTCCGACGACCCCGCGCCGT
GGTATGCGGGAAAAGGCAAATCGGCTAAAAATACGTCTAAAAAATGA

Domains

Predicted by InterproScan.

Sulfatase (Sulfatase)

(232-495)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] L A Snyder et al. (2001) A putatively phase variable gene (dca) required for natural competence in Neisseria gonorrhoeae but not Neisseria meningitidis is located within the division cell wall (dcw) gene cluster. Journal of Bacteriology 183(4):1233-41. [PMID: 11157935]