Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACNSPE_RS03975 Genome accession   NZ_CP183326
Coordinates   839176..841542 (+) Length   788 a.a.
NCBI ID   WP_419393658.1    Uniprot ID   -
Organism   Cytobacillus praedii strain MM2025     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 834176..846542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNSPE_RS03960 (ACNSPE_03960) - 835039..835335 (+) 297 WP_057764782.1 post-transcriptional regulator -
  ACNSPE_RS03965 (ACNSPE_03965) secDF 835554..837815 (+) 2262 WP_057764783.1 protein translocase subunit SecDF -
  ACNSPE_RS03970 (ACNSPE_03970) - 837917..838846 (+) 930 WP_419393657.1 cation diffusion facilitator family transporter -
  ACNSPE_RS03975 (ACNSPE_03975) recJ 839176..841542 (+) 2367 WP_419393658.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACNSPE_RS03980 (ACNSPE_03980) - 841533..842045 (+) 513 WP_057764787.1 adenine phosphoribosyltransferase -
  ACNSPE_RS03985 (ACNSPE_03985) - 842280..844478 (+) 2199 WP_057764788.1 RelA/SpoT family protein -
  ACNSPE_RS03990 (ACNSPE_03990) dtd 844491..844931 (+) 441 WP_057764790.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 788 a.a.        Molecular weight: 88305.81 Da        Isoelectric Point: 4.7923

>NTDB_id=1105281 ACNSPE_RS03975 WP_419393658.1 839176..841542(+) (recJ) [Cytobacillus praedii strain MM2025]
MLHSKTRWIVQNSDEEKENLLVQELNITPLVASLLVNRGLETPDLARSFLFDQKQKFHDPFLLSDMDAAVSRIKEAIENQ
EAILIFGDYDADGVTSTTVMMKTLQELGANVEFYIPNRFTEGYGPNIPAFQHAAETGIKLIITVDTGIAAVNEAMAAKEL
GLDLIITDHHEPGPELPDALAIIHPKLENSEYPFRDLAGVGVAFKLAHALLGRVPEHLLEIAAIGTIADLVSLKGENRLI
AKKGIKKFQSTSNVGLKALLKLNKVEPNSINEETIGFLIAPRLNAAGRLESADPAVDLLLTEDSLEAEAIAEEIEQLNKD
RKTIVNEITEQAIREVEENYPVNENAVLVIGREGWNAGVIGIVASKLVETFYRPAIVLSYDVEKGLAKGSARSIAGFDLF
KNLSECREILPHFGGHPMAAGMTLKLDDVNDLRSRLNAAAKDQLSEEDFIPVTQLDGEIALHDIHLSSLEELQMLSPYGM
DNPKPKIMIKNANISAIRKIGSDQNHLKMTIENDGHTLDGVGFGLGSLFDHISPSSQISVIGELSINEWNNIRKPQMFLQ
DIAIDAWQLFDIRGVKRIESLVELIPKDRSKWIVFNQENMSKFQKYTNDKLITVSSVEEASALNIEKENVVLIDLPDSKE
LLAAVFAGKNPARVYAHFYNENDAYFSTIPTRDHFKWFYALLVKKGPFDLKKYGDDIAKFKGWSRQTIDFMSKVFFELDF
VKIENGFISVNKATQKRDLTDSLTYQKVLAQYTLERDMVYSSYQQLKGWFDEVIRGSREIEEEMKEWT

Nucleotide


Download         Length: 2367 bp        

>NTDB_id=1105281 ACNSPE_RS03975 WP_419393658.1 839176..841542(+) (recJ) [Cytobacillus praedii strain MM2025]
ATGTTACATTCAAAAACACGATGGATTGTTCAGAATTCAGATGAAGAAAAGGAAAATCTACTTGTACAAGAATTAAATAT
TACGCCTCTCGTGGCTTCGCTCCTTGTTAATCGTGGATTAGAAACGCCGGATTTAGCACGGTCTTTTTTATTTGATCAAA
AGCAGAAATTTCACGATCCTTTTTTATTATCAGATATGGATGCTGCGGTATCAAGAATAAAAGAGGCAATCGAGAATCAG
GAAGCAATTTTAATTTTTGGAGACTATGACGCAGATGGTGTAACGAGTACGACAGTGATGATGAAGACTCTTCAAGAGCT
TGGTGCAAATGTAGAATTTTATATACCAAACCGCTTTACAGAAGGGTATGGACCCAATATACCAGCATTTCAGCATGCGG
CAGAAACAGGTATTAAACTTATCATTACAGTTGATACAGGTATAGCAGCTGTAAATGAGGCAATGGCCGCAAAAGAGCTA
GGGCTTGATCTTATAATTACTGACCATCATGAACCAGGACCTGAGCTGCCTGATGCATTGGCAATTATTCACCCGAAGCT
TGAAAATAGTGAATATCCATTCCGTGATTTAGCCGGTGTGGGGGTAGCATTTAAATTAGCTCATGCTTTATTAGGCAGGG
TTCCAGAACATTTGCTTGAAATTGCTGCAATTGGTACAATTGCTGATCTTGTTTCTCTAAAAGGAGAAAACCGCTTAATT
GCTAAAAAGGGGATTAAAAAATTTCAATCGACATCAAATGTTGGTTTAAAAGCATTATTAAAACTAAATAAAGTAGAGCC
TAACTCAATAAATGAAGAAACAATTGGTTTTCTCATCGCACCTCGCTTGAATGCGGCAGGAAGATTGGAAAGTGCTGATC
CAGCAGTGGATTTGCTGTTAACGGAAGATAGTCTTGAAGCAGAGGCAATTGCAGAGGAAATTGAGCAATTAAATAAGGAT
CGAAAGACAATAGTCAATGAGATTACCGAGCAGGCCATTCGTGAAGTTGAGGAAAATTATCCAGTGAATGAAAATGCTGT
TCTTGTTATTGGAAGAGAAGGCTGGAATGCTGGAGTAATTGGCATTGTCGCATCAAAGCTCGTAGAAACTTTCTACCGGC
CTGCGATTGTACTAAGCTATGATGTAGAAAAGGGTTTGGCAAAAGGATCTGCTAGAAGTATAGCAGGCTTTGACCTCTTT
AAAAATCTTTCAGAATGCAGAGAGATTTTGCCTCATTTTGGCGGACATCCGATGGCAGCTGGGATGACATTGAAGCTTGA
TGATGTGAATGATCTTCGGAGCAGACTCAATGCTGCCGCAAAAGATCAGTTATCAGAGGAGGATTTTATTCCTGTAACCC
AACTTGATGGAGAAATCGCCTTACATGATATTCATTTGTCATCTTTAGAAGAACTGCAAATGCTTTCTCCATATGGAATG
GATAACCCAAAACCAAAAATAATGATTAAAAATGCCAATATATCTGCAATAAGAAAGATTGGTTCGGACCAGAATCATTT
GAAGATGACGATTGAAAATGATGGACATACATTAGATGGAGTCGGTTTTGGTCTTGGTTCATTATTTGACCACATTTCTC
CAAGTTCGCAAATTTCGGTTATCGGGGAGCTATCGATTAATGAATGGAATAATATTCGCAAGCCGCAAATGTTTCTCCAA
GATATAGCTATTGACGCATGGCAGCTATTTGATATTAGGGGAGTAAAGCGGATTGAAAGTTTAGTCGAGCTCATCCCAAA
GGATCGTTCAAAATGGATTGTTTTTAATCAAGAGAATATGAGTAAATTTCAAAAGTATACAAACGATAAGTTAATAACGG
TTAGTTCTGTAGAGGAAGCCTCTGCACTAAACATTGAGAAAGAAAATGTGGTTTTGATCGATCTTCCAGACTCTAAGGAA
TTATTAGCTGCAGTATTTGCTGGGAAAAATCCAGCAAGAGTGTACGCACATTTTTATAATGAAAATGACGCATATTTTAG
TACGATTCCAACAAGAGATCATTTTAAATGGTTCTATGCTTTATTAGTAAAGAAGGGACCTTTTGATCTAAAAAAATATG
GAGATGACATTGCTAAATTTAAAGGCTGGAGCAGACAAACAATTGACTTCATGTCAAAGGTGTTTTTTGAACTTGATTTT
GTTAAAATAGAAAATGGATTCATTTCAGTTAATAAAGCAACACAAAAACGTGATTTAACTGATTCATTAACATACCAGAA
GGTGCTGGCACAGTATACACTTGAGAGGGATATGGTGTATTCTTCCTATCAGCAATTAAAAGGATGGTTTGACGAAGTGA
TCCGAGGTTCACGTGAAATTGAGGAGGAAATGAAAGAATGGACTTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(456-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHHA1 domain (DHHA1)

(315-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.076

100

0.551

Multiple sequence alignment