Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ACNSPI_RS08525 Genome accession   NZ_CP183317
Coordinates   1647421..1648491 (+) Length   356 a.a.
NCBI ID   WP_000775708.1    Uniprot ID   -
Organism   Staphylococcus aureus strain USA300     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1642421..1653491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNSPI_RS08495 (ACNSPI_08495) - 1642844..1644307 (+) 1464 WP_001017867.1 rhomboid family protein -
  ACNSPI_RS08500 (ACNSPI_08500) - 1644288..1644491 (+) 204 WP_000087561.1 YqgQ family protein -
  ACNSPI_RS08505 (ACNSPI_08505) - 1644488..1645474 (+) 987 WP_000161314.1 ROK family glucokinase -
  ACNSPI_RS08510 (ACNSPI_08510) - 1645474..1645803 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACNSPI_RS08515 (ACNSPI_08515) - 1645800..1646423 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACNSPI_RS08520 (ACNSPI_08520) comGA 1646475..1647449 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  ACNSPI_RS08525 (ACNSPI_08525) comGB 1647421..1648491 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACNSPI_RS08530 (ACNSPI_08530) comGC 1648505..1648816 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACNSPI_RS08535 (ACNSPI_08535) comGD 1648794..1649240 (+) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACNSPI_RS08540 (ACNSPI_08540) comGE 1649227..1649526 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACNSPI_RS08545 (ACNSPI_08545) comGF 1649444..1649941 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACNSPI_RS08550 (ACNSPI_08550) - 1650038..1650184 (+) 147 WP_001789879.1 hypothetical protein -
  ACNSPI_RS08555 (ACNSPI_08555) - 1650174..1650698 (+) 525 WP_001015117.1 shikimate kinase -
  ACNSPI_RS08560 (ACNSPI_08560) gcvT 1650857..1651948 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACNSPI_RS08565 (ACNSPI_08565) gcvPA 1651968..1653314 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41364.77 Da        Isoelectric Point: 10.0092

>NTDB_id=1105256 ACNSPI_RS08525 WP_000775708.1 1647421..1648491(+) (comGB) [Staphylococcus aureus strain USA300]
MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1105256 ACNSPI_RS08525 WP_000775708.1 1647421..1648491(+) (comGB) [Staphylococcus aureus strain USA300]
GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997

Multiple sequence alignment