Detailed information NTG0001105

Experimentally validated

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: NGFG_RS10640
• Genome accession: NC_022240
• Coordinates: 1959142..1962229 (-)
• Length: 1028 a.a.
• NCBI ID: Protein_1991
• Uniprot ID: -
• Organism: Neisseria gonorrhoeae MS11
• Function: assembly of type IV pilus DNA binding and uptake

Function

DNA uptake deficiency of defined piliated pilC1,2 double mutants can be complemented by expression of a cloned pilC2 gene in trans. The PilC defect can also be restored by the addition of purified PilC protein, or better, pili containing PilC protein, to the mutant gonococci. Our data suggest that the two phase-variable Pil proteins act on the bacterial cell surface and cooperate in DNA recognition and/or outer membrane translocation.

Genomic Context

Location: 1954142..1967229

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NGFG_RS10625 (NGFG_01978)	yccS	1954434..1956584 (+)	2151	WP_003694501.1	YccS family putative transporter	-
NGFG_RS10630 (NGFG_02247)	-	1957514..1957798 (-)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
NGFG_RS10640 (NGFG_01977)	pilC	1959142..1962229 (-)	3088	Protein_1991	PilC family type IV pilus tip adhesin	Machinery gene
NGFG_RS10645	-	1962918..1963181 (+)	264	WP_017147189.1	hypothetical protein	-
NGFG_RS10650 (NGFG_01975)	gnd	1963261..1964709 (+)	1449	WP_003688061.1	decarboxylating NADP(+)-dependent phosphogluconate dehydrogenase	-
NGFG_RS10655 (NGFG_01974)	waaA	1964772..1966043 (+)	1272	WP_017147188.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
NGFG_RS10660 (NGFG_01973)	-	1966084..1966533 (+)	450	WP_003692085.1	CopD family copper resistance protein	-

Sequence

Protein

• Length: 1028 a.a.
• Molecular weight: Da
• Isoelectric Point:

>NTDB_id=1105 NGFG_RS10640 Protein_1991 1959142..1962229(-) (pilC) [Neisseria gonorrhoeae MS11]
None

Nucleotide

• Length: 3088 bp

>NTDB_id=1105 NGFG_RS10640 Protein_1991 1959142..1962229(-) (pilC) [Neisseria gonorrhoeae MS11]
ATGAATAAAACTTTGAAACGGCAGGTTTTCCGCCATACCGCGCTTTATGCCGCCATCTTGATGTTTTCCCATACCGGCGG
GGGGGGGGGCGATGGCGCAAACCCATCAATACGCTATTATCATGAACGAGCGAAACCAGCCCGAGGTAAAGCAGAATGTG
CCATCTTCAATAAAGGACAAAGACAGGAGGCGCGAATATACTTATTATACGCACAGAACAGGAGGAGGCTCTGTCTCATT
CAACAATAACGATACCCTTGTTTCCCAACAAAGCGGTACTGCCGTTTTTGGCACAGCCACCTACCTGCCGCCCTACGGCA
AGGTTTCCGGTTTTGATGCCGACGCTCTGAAAGAGCGCAACAATGCCGTTGATTGGATTCGTACCACCCGCATCGCGCTG
GCAGGCTACTCCTACATCGACGTCATATGCAGAAGCTACACAGGCTGTCCCAAACTTGTCTATAAAACCCGATTTACCTT
CGGTCAACAAGGGTTGAAAAGAAAGGCAGGCAGCAAGCTGGATATATACGAAGACAAAAGCCGCGAAAATTCGCCCATTT
ACAAATTGTCGGATTATCCTTGGTTGGGCGTATCTTTCAATTTGGGCAGCGAGAATACCGTCCAAAATAGCAAATTATTC
AACAAATTGATATCTTCTTTTAGAGAAGGCAATAATAATCAAACCATCGTCTCTACGACAGAAGGCAACCCTATTTCCCT
TGGCGACCGGCAGCGCGAACATACCGCCGTGGCCTATTATCTGAACGCCAAACTGCACCTGCTGGACAAAAAAGGGATTG
AAGATATCGCCCAAGGCAAAATAGTGGATTTGGGTATCTTGAAACCGCACGTCGAGACGACAGGACGAAGCTTGCTAGAT
TTTTGGGCTAGGTGGGACATTAAAGATACCGGGCAGATTCCGGTCAAGCTCGGCCTGCCGCAAGTCAAAGCAGGCCGCTG
CACCAACAAACCGAACCCCAATAATAATACCAAAGCCCCTTCGCCGGCACTGACCGCCCCCGCGCTGTGGTTCGGACCCG
GGCAAGATGGTAAGGCGGAGATGTATTCCGCTTCGGTTTCCACCTACCCCGACAGTTCGAGCAGCCGCATCTTCCTCCAA
GAGCTGAAAACTCAAACCGAACCCGGCAAACCCGGCCGCTATTCCCTCAAATCTTTGAATGATGGTGAGATTAAAAGTCG
ACAGCCGAGTTTCAACGGGCGGCAAACAATCATCCGATTGGATGACGGCGTACATTTGATCAAACTGAATGGAAGCAAGG
ATGAGGTCGCCGCTTTTGTCAATTTAAATGGAAACAACACCGGCAAAAACGACACTTTCGGCATTGTTAAGGAAGCGAAC
GTCAATCTTGACGCCGACGAGTGGAAAAAAGTGCTGCTGCCTTGGACGGTTCGGGGTCCCGATAATGACAATAAATTTAA
ATCAATTAACCAAAAACCAGAAAAATACAGCCAAAGATACCGCATCCGCGACAACAACGGCAATCGCGATTTGGGCGACA
TCGTCAACAGCCCGATTGTCGCGGTCGGCGGGTATTTGGCAACCGCCGCGAACGACGGGATGGTGCATATCTTCAAAAAA
AACGGCGGCAGTGATGAACGCAGCTACAATCTGAAGCTCAGCTACATCCCCGGCACGATGCCGCGCAAGGATATTCAAAG
CCAAGAATCCACCCTTGCCAAAGAGCTGCGCGCCTTTGCCGAAAAAGGCTATGTGGGCGACCGCTACGGCGTGGACGGCG
GCTTTGTCTTGCGCCAAGTCGAACTGAGCGGGCAAAAACACGTGTTTATGTTCGGCGCGATGGGTTTTGGCGGCAGGGGC
GCGTATGCCTTGGATTTAAGCAAAATCAACGGAAATTATCCGGCCGCCGCCCCCCTGTTTGATGTCAAAGATGGCGATAA
TAACGGCAAAAATCGCGTGAAAGTGGAATTAGGCTACACCGTCGGTACGCCGCAAATCGGCAAAATCCGCAACGGCAAAT
ACGCCGCCTTCCTCGCCTCCGGTTATGCGGCTAAAAAAATTGACGACTCAACAAATAAAACCGCGCTGTATGTATATGAT
TTGAAAGACACCTTAGGTACGCCGATTGCAAAAATCGAAGTGAAGGACGGCAAAGGCGGGCTTTCGTCCCCCACGCTGGT
GGATAAAGATTTGGACGGCACGGTCGATATCGCCTATGCCGGCGACCGGGGCGGCAATATGTACCGCTTTGATTTGAGCA
ATTCCGATTCTAGTAAATGGTCTGCAAAGGTTATTTTCGAAGGCGACAAGCCGATTACCTCCGCGCCCGCCGTTTCCCGA
CTGGCAGACAAACGCGTCGTCATCTTCGGTACGGGCAGCGATTTGACCGAAGATGATGTACTGAATACGGGCGAACAATA
TATTTACGGTATCTTTGACGACGATAAGGGGACGGTTAAGGTAACGGTACAAAACGGCACGGCAGGCGGGCTGCTCGAGC
AACACCTTACTCAGGAAAATAAAACATTATTCCTGAACAAGAGATCCGACGGTTCGGGCAGCAAGGGCTGGGCGGTGAAA
TTGAGGGAAGGAGAACGCGTTACCGTCAAACCGACCGTGGTATTGCGTACCGCCTTCGTAACCATCCGCAAATATAACGA
CGGCGGCTGCGGCGCGGAAACCGCCATTTTGGGCATCAATACCGCCGACGGCGGCGCATTGACTCCGAGAAGCGCGCGCC
CGATTGTGCCGGATCACAATTCGGTTGCGCAATATTCCGGCCATAAGACAACCTCCAAAGGCAAATCCATCCCTATAGGT
TGTATGGACAAAGACGGTAAAACCGTCTGCCCGAACGGATATGTTTACGACAAGCCGGTTAATGTGCGTTATCTGGATGA
AACGGAAACAGACGGATTTTCAACGACGGCGGACGGCGATGCGGGCGGCAGCGGTATAGACCCCGCCGGCAGGCGTCCCG
GCAAAAACAACCGCTGCTTCTCCAAAAAAGGGGTGCGCACCCTGCTGATGAACGATTTGGACAGCTTGGATATTACCGGC
CCGATGTGCGGTATCAAACGCTTAAGCTGGCGCGAAGTCTTCTTCTGA

Domains

No domain identified.

References

• [1]: Hanne C Winther-Larsen et al. (2005) A conserved set of pilin-like molecules controls type IV pilus dynamics and organelle-associated functions in Neisseria gonorrhoeae. Molecular Microbiology 56(4):903-17. [PMID: 15853879]
• [2]: Marianne Bäckman et al. (1998) The phase-variable pilus-associated protein PilC is commonly expressed in clinical isolates of Neisseria gonorrhoeae, and shows sequence variability among strains. Microbiology (Reading, England) 144 ( Pt 1):149-156. [PMID: 9467907]
• [3]: R R Ryll et al. (1997) PilC of Neisseria meningitidis is involved in class II pilus formation and restores pilus assembly, natural transformation competence and adherence to epithelial cells in PilC-deficient gonococci. Molecular Microbiology 23(5):879-92. [PMID: 9076726]
• [4]: T Rudel et al. (1995) Role of pili and the phase-variable PilC protein in natural competence for transformation of Neisseria gonorrhoeae. Proceedings of The National Academy of Sciences of The United States of America 92(17):7986-90. [PMID: 7644525]