Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   NGFG_RS00285 Genome accession   NC_022240
Coordinates   53354..56507 (-) Length   1050 a.a.
NCBI ID   Protein_51    Uniprot ID   -
Organism   Neisseria gonorrhoeae MS11     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

DNA uptake deficiency of defined piliated pilC1,2 double mutants can be complemented by expression of a cloned pilC2 gene in trans. The PilC defect can also be restored by the addition of purified PilC protein, or better, pili containing PilC protein, to the mutant gonococci. Our data suggest that the two phase-variable Pil proteins act on the bacterial cell surface and cooperate in DNA recognition and/or outer membrane translocation.


Genomic Context

Location: 48354..61507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS00260 (NGFG_00184) - 49619..50008 (-) 390 WP_003687278.1 endonuclease domain-containing protein -
  NGFG_RS00270 (NGFG_00186) - 50171..50550 (-) 380 Protein_49 VOC family protein -
  NGFG_RS00275 (NGFG_00187) carA 50604..51737 (-) 1134 WP_003694721.1 glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit -
  NGFG_RS00285 (NGFG_00188) pilC 53354..56507 (-) 3154 Protein_51 PilC family type IV pilus tip adhesin Machinery gene
  NGFG_RS14945 (NGFG_02257) - 56642..56830 (-) 189 WP_154236026.1 hypothetical protein -
  NGFG_RS00300 (NGFG_00189) apbC 57946..59025 (-) 1080 WP_003687290.1 iron-sulfur cluster carrier protein ApbC -
  NGFG_RS00310 (NGFG_00190) - 59399..59908 (+) 510 WP_003687293.1 protein disulfide oxidoreductase -
  NGFG_RS00320 (NGFG_00192) nadR 60249..60689 (+) 441 WP_003694725.1 MarR family adhesin repressor NadR -
  NGFG_RS00325 (NGFG_00193) hpaC 60804..61304 (+) 501 WP_003687298.1 4-hydroxyphenylacetate 3-monooxygenase, reductase component -

Sequence

Protein


Download         Length: 1050 a.a.        Molecular weight: Da        Isoelectric Point:

>NTDB_id=1104 NGFG_RS00285 Protein_51 53354..56507(-) (pilC) [Neisseria gonorrhoeae MS11]
None

Nucleotide


Download         Length: 3154 bp        

>NTDB_id=1104 NGFG_RS00285 Protein_51 53354..56507(-) (pilC) [Neisseria gonorrhoeae MS11]
ATGAATAAAACTTTAAAAAGGCGGGTTTTCCGCCATACCGCGCTTTATGCCGCCATCTTGATGTTTTCCCATACCGGGCG
GGGGGGGGGGGCGGCAGGCGCAAACCCGTAAATACGCTATTATCATGAACGAGCGAAAGCAGCCCGAGGTAAAGTGGGAG
GGTCAATATAGTCAATCAACATTAAAGGACAAAGGCAGGGAGCGGACATTTAGCCATACGAGCCAGAGAAACTGGAACGG
CCAACAAAACAATTTTATCTCATTCAACAATAGCGATGAGCTTGTTTCCCGACAAAGCGGTACTGCCGTTTTTGGCACAG
CCACCTACCTGCCGCCCTACGGCAAGGTTTCCGGTTTTGATGCCGACGGGCTGAAAAAGCGCGGCAATGCCGTGAATTGG
ATTCGTACCACCCGGCCCGGGCTGGCAGGCTACATCTACACCGGCGTCATATGCAGAGACACAGGGCAATGCCCCGAACT
TGTCTATGAGACCAAATTTTCCTTCGACGGCATCGATTTGGCAAAAGGGGGAAACCGAAAGCTGGATAGGCACCCGGACC
CAAGCCGCGAAAATTTGCCCATTTACAAATTGAAGGATCATCCATGGTTGGGCGTATCTTTCAATTTGGGCGGCGAGGGT
ACCGCCAAAGATGGCAGATCATCCAGCAGATGGATATCTTCTTTTAGTGAAGACAATAATAATCAAACCATCGTCTTTAC
GACACGAGGCCACCCTATTTCCCTTGGCGACTGGCAGCGCGAAAGTACCGCCATGGCCTATTATCTGGACGCCAAACTGC
ACCTGCTGGATAAAACACAGATTGAAAATATCGCGCCAGGCAAAACAGTGAATTTGGGCATCTTGAGACCGCGCGTCGAG
GCAAAGGTAAGGCGGAAGTGGGATCTGCTAAATTTTTGGGCTAAGTGGGACATTAAAGATACCGGGCAGATTCCGGTCAA
GCTCGGCCTGCCGGAAGTCAAAGCAGGCCGCTGCATCAACAAACCGAACCCCAATCCCAAATCAGCCCTTTCGCCGGCAC
TGACCGCCCCCGCGCTGTGGTTCGGCCCTGTGCAAAATGGCAAGGTGCAGATGTATTCCGCTTCGGTTTCCACCTACCCC
GGCAGCTCGAGCAGCCGCATCTTCCTCCAAGAGCTGAAAACTAAAACCGACCCCGCCCGGCCCGGCCGGCATTCCCTCGC
CGCTTTGAATGCGCAGGATATCAAATCCCGCGAGCCGAATTTCAACTCAAGGCAGACCGTCATCCGATTGCCGGGCGGCG
TGTACCAGATCGCCCCGGGCAATAGCGGCCGGGTCGCGGGTTTTAATGGCAATGACGGCAAAAACGACACTTTCGGCATC
TACAAGGACAGGCTCGTCACACCTGAGGTCGGCGAGTGGAGCGAAGTGCTGCTGCCTTGGACGGCCCGGTATTACGGTAA
TGACGATATATTTAAAACATTCAACCAACCAAACAGCAAAACACAAAACGGCAAAAAACAATACAGCCAAAAATACCGCA
TCCGCACAAAAGAAAATGACAATGACAAACCCCGCGATTTGGGCGACATCGTCAACAGCCCGATTGTCGCGGTCGGCGGG
TATCTGGCAACTTCTGCCAACGACGGGATGGTGCATATCTTCAAAAAAACCGGCACGGATGAACGCAGCTACAATCTGAA
GCTCAGCTACATCCCCGGTACGATGGAGCGTAAGGATATTGAAGGCAATGACTCCGACCTCGCCAAAGAGCTGCGCACCT
TTGCCGAAAAAGGCTATGTGGGCGACCGCTACGGCGTGGACGGCGGCTTTGTCTTGCGCCGCATTACAGATGACCAAGAC
AAGCAAAAACATTTCTTTATGTTTGGTGCGATGGGCCTGGGCGGCAGAGGCGCGTATGCCTTGGATTTAAGCAAAATCGA
CAGCAGCAACCTGACCGGCGTTTCCATGTTTGATGTCCAAAACGACAAAAATAACAATAACAATAAGAATGACAATAATC
GCGTGAAATTAGGCTACACCGTCGGTACGCCGCAAATCGGCAAAACCCAAAACGGCAAATACGCCGCCTTCCTCGCTTCC
GGTTATGCGGCTAAAAATATTGGCAGCGGCGATAATACAACCGCGCTGTATGTGTATGATTTGGAAAACACCAGTGGTAG
TCTGATTAAAAAAATCGAAGCACCCGGCGGCAAAGGCGGGCTTTCGTCCCCCACGCTGGTGGATAAAGATTTGGACGGCA
CGGTCGATATCGCCTATGCCGGCGACCGGGGCGGCAATATGTACCGCTTTGATTTGAGCAATTCCGATTCTAGTAAATGG
TCTGCAAAGGTTATTTTCGAAGGCGACAAGCCGATTACCTCCGCGCCCGCCGTTTCCCGACTGGCAGACAAACGCGTGGT
TATCTTCGGCACGGGCAGCGATTTGAGTGAACAGGATGTACTGGATACGGACAAACAATATATTTACGGTATCTTTGACG
ACGATAAGTCGACGGTTAATGTAAAGGTAACAAACGGCACGGGAGGCGGGCTGCTCGAGCAAGTGCTTAAAGAGGAAAGT
AAAACCTTATTCCTGAGCAATAATAAGGCATCCGGCGGATCGGCCGATAAAGGGTGGGTAGTGAAATTGAGGGAAGGAGA
ACGCGTTACCGTCAAACCGACCGTGGTATTGCGTACCGCCTTTGTCACCATCCGCAAATATACGGATACGGACAAATGTG
GCGCGCAAACCGCCATTTTGGGCATCAATACCGCCGACGGCGGCGCATTGACTCCGAGAAGCGCGCGCCCGATTGTGCCG
GATCACAATTCGGTTGCGCAATATTCCGGCCATCAGAAAATGAACGGCAAGTCCATCCCCATAGGCTGCATGTGGAAAAA
CAGCAAAACCGTCTGCCCGAACGGATATGTTTACGACAAACCGGTTAATGTGCGTTACCTGGACGAAAAGAAAACAGACG
ATTTCCCCGTCACGGCAGACGGTGATGCAGGCGGCAGCGGAACATTCAAAGAGGGTAAAAAACCCGCCCGCAATAACCGG
TGCTTCTCCGGAAAAGGTGTGCGCACCCTGCTGATGAACGATTTGGACAGCTTGGATATTACCGGCCCGATGTGCGGTAT
CAAACGCTTAAGCTGGCGCGAAGTCTTCTTCTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Hanne C Winther-Larsen et al. (2005) A conserved set of pilin-like molecules controls type IV pilus dynamics and organelle-associated functions in Neisseria gonorrhoeae. Molecular Microbiology 56(4):903-17. [PMID: 15853879]
[2] Marianne Bäckman et al. (1998) The phase-variable pilus-associated protein PilC is commonly expressed in clinical isolates of Neisseria gonorrhoeae, and shows sequence variability among strains. Microbiology (Reading, England) 144 ( Pt 1):149-156. [PMID: 9467907]
[3] R R Ryll et al. (1997) PilC of Neisseria meningitidis is involved in class II pilus formation and restores pilus assembly, natural transformation competence and adherence to epithelial cells in PilC-deficient gonococci. Molecular Microbiology 23(5):879-92. [PMID: 9076726]
[4] T Rudel et al. (1995) Role of pili and the phase-variable PilC protein in natural competence for transformation of Neisseria gonorrhoeae. Proceedings of The National Academy of Sciences of The United States of America 92(17):7986-90. [PMID: 7644525]