Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACM1PF_RS05160 Genome accession   NZ_CP182236
Coordinates   1082618..1083736 (-) Length   372 a.a.
NCBI ID   WP_416672813.1    Uniprot ID   -
Organism   Egbenema sp. FJBBOT/2019     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1077618..1088736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM1PF_RS05140 (ACM1PF_05140) - 1078666..1080093 (-) 1428 WP_416672804.1 type II secretion system protein GspD -
  ACM1PF_RS05145 (ACM1PF_05145) - 1080096..1080890 (-) 795 WP_416672806.1 AMIN domain-containing protein -
  ACM1PF_RS05150 (ACM1PF_05150) - 1081005..1081799 (-) 795 WP_416672808.1 pilus assembly protein PilO -
  ACM1PF_RS05155 (ACM1PF_05155) - 1081796..1082605 (-) 810 WP_416672811.1 PilN domain-containing protein -
  ACM1PF_RS05160 (ACM1PF_05160) pilM 1082618..1083736 (-) 1119 WP_416672813.1 type IV pilus assembly protein PilM Machinery gene
  ACM1PF_RS05165 (ACM1PF_05165) nth 1085079..1085744 (-) 666 WP_416672817.1 endonuclease III -
  ACM1PF_RS05170 (ACM1PF_05170) rseP 1085841..1086932 (-) 1092 WP_416672819.1 RIP metalloprotease RseP -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40444.22 Da        Isoelectric Point: 4.5212

>NTDB_id=1100949 ACM1PF_RS05160 WP_416672813.1 1082618..1083736(-) (pilM) [Egbenema sp. FJBBOT/2019]
MTSGLKNLFSRKTKGIGVELAPDRVNVSRLKKKGQGFQLTSLASAEVPDGIFEEGQILDSAGMAEIIQSVLAEAKLKVKH
AATAIPGSRDTVTRIIPVPAELDDRELREMVLNQEAGLYLPFPREEADVDYQKLGFFVDEDGIEKVQVLLVATRKEVTDS
YLETFRQAGLVVDVLEISSFSLIRTIRQQLRQFTPQEAAAIVDIQFESTEISIVVDGVPQFSRTVPIGTYQVQSALSRAM
NLPPTRNTELLQGMTVPVTPIDSMSTAAPRPGGTNPGTAAMLRILGELADELRRSIDFYHNQGENTEVAQLLLAGPGGAI
GQLDEFFSQRLSLPASQVDPIAALSLEVEQEIPPIQRAGLGVVLGLGLREAW

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1100949 ACM1PF_RS05160 WP_416672813.1 1082618..1083736(-) (pilM) [Egbenema sp. FJBBOT/2019]
GTGACTAGTGGTCTCAAGAATCTATTCTCAAGAAAGACAAAAGGGATCGGTGTAGAACTGGCTCCCGATCGTGTCAACGT
TTCTCGTCTCAAGAAAAAAGGTCAGGGTTTTCAACTGACCAGCCTGGCTTCTGCTGAGGTTCCTGATGGCATCTTTGAAG
AGGGGCAAATTTTAGACTCTGCGGGAATGGCAGAGATCATTCAGTCCGTACTGGCTGAAGCAAAGCTCAAGGTGAAACAC
GCTGCCACAGCTATCCCCGGAAGTCGAGATACCGTGACCCGGATTATTCCTGTTCCGGCTGAATTGGACGATCGAGAACT
GCGGGAAATGGTTTTGAACCAGGAAGCAGGTCTGTATCTTCCTTTTCCCCGTGAAGAGGCCGATGTGGATTACCAGAAGT
TAGGGTTCTTTGTCGATGAAGACGGGATTGAGAAAGTTCAGGTCTTGCTTGTAGCAACGCGAAAGGAAGTTACCGATTCT
TACCTGGAAACGTTCCGGCAGGCAGGGCTGGTTGTAGATGTTTTAGAAATCAGCAGTTTTTCCCTCATTCGAACGATTCG
GCAGCAACTCCGCCAATTTACTCCCCAGGAGGCAGCGGCGATCGTTGATATTCAGTTTGAAAGTACTGAAATTTCCATTG
TCGTAGATGGGGTGCCCCAGTTCTCGCGGACGGTTCCCATCGGAACCTATCAGGTGCAAAGTGCCCTCAGCCGAGCCATG
AACTTGCCTCCTACCCGCAATACAGAACTGCTCCAGGGGATGACGGTTCCCGTTACCCCGATTGACAGTATGAGTACAGC
GGCTCCCAGACCAGGTGGCACGAACCCTGGAACGGCTGCCATGCTGCGGATTTTGGGCGAACTTGCCGATGAACTGCGTC
GTTCGATTGACTTTTACCACAATCAGGGTGAGAACACGGAGGTCGCGCAATTATTGCTGGCTGGCCCCGGTGGCGCAATT
GGACAACTCGATGAATTTTTCTCGCAGCGATTAAGTCTACCTGCCAGTCAGGTTGATCCGATCGCAGCCCTATCGTTAGA
GGTGGAGCAAGAGATTCCACCGATTCAGCGAGCGGGTTTAGGTGTGGTGTTGGGGTTAGGTTTACGGGAGGCTTGGTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

63.073

99.731

0.629

Multiple sequence alignment