Detailed information NTG0001097

Experimentally validated

Overview

• Name: rho
• Type: Unclear
• Locus tag: KZH42_RS03865
• Genome accession: NZ_CP079941
• Coordinates: 781037..782296 (-)
• Length: 419 a.a.
• NCBI ID: WP_002219650.1
• Uniprot ID: Q9K0I3
• Organism: Neisseria meningitidis strain C311
• Function: require for natural transformation Unclear

Function

rho mutant has a substantially reduced transformation frequency.

Genomic Context

Location: 776037..787296

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KZH42_RS03850 (KZH42_03850)	-	776955..778250 (+)	1296	WP_002225537.1	FAD-binding oxidoreductase	-
KZH42_RS03855 (KZH42_03855)	-	778495..779799 (-)	1305	WP_002222836.1	ammonium transporter	-
KZH42_RS03860 (KZH42_03860)	-	780327..780731 (-)	405	WP_002222835.1	DDE transposase	-
KZH42_RS03865 (KZH42_03865)	rho	781037..782296 (-)	1260	WP_002219650.1	transcription termination factor Rho	Unclear
KZH42_RS03870 (KZH42_03870)	ppsA	782516..784900 (-)	2385	WP_002222834.1	phosphoenolpyruvate synthase	-
KZH42_RS03875 (KZH42_03875)	-	785312..786133 (+)	822	WP_002217768.1	pyruvate, water dikinase regulatory protein	-
KZH42_RS03880 (KZH42_03880)	-	786372..787034 (-)	663	WP_002222833.1	HAD family hydrolase	-

Sequence

Protein

• Length: 419 a.a.
• Molecular weight: 47306.39 Da
• Isoelectric Point: 6.6458

>NTDB_id=1097 KZH42_RS03865 WP_002219650.1 781037..782296(-) (rho) [Neisseria meningitidis strain C311]
MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMKKGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIY
VSPTQIRRFNLHTGDTIEGSVRVPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTEMSRSVRGEVVSSTFDEPAQRHV
QVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNTVVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATAL
VETGSRMDDVIYEEFKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDEIEAAEFLI
GKIKASKNNDDFFELMRGK

Nucleotide

• Length: 1260 bp

>NTDB_id=1097 KZH42_RS03865 WP_002219650.1 781037..782296(-) (rho) [Neisseria meningitidis strain C311]
ATGCACGTCTCCGAATTACAAACCCTGCACATTTCCAAACTCTTAGAATTGGCGGAAGAACACGGCATCGAAAACGCCAA
CCGATTCCGCAAACAAGACCTCGTATTTGCCATCGTCCGCCAGATGATGAAAAAAGGCGAGGGTTTCACCTGCTCCGGCA
CGCTTGAAATCCTGCCCGACGGCTTCGGCTTCCTCCGCAGCGCGGACACGTCCTATCTTGCCGGCCCCGACGACATCTAT
GTCTCGCCCACCCAAATCCGCCGCTTCAACCTGCATACGGGCGACACCATCGAAGGAAGCGTGCGCGTCCCAAAAGACAA
CGAACGCTATTTTGCCCTGGTCAGGCTTGATACCATCAACGGCGACCACCCGGAAGTATGCCGCCATAAAATCCTGTTTG
AAAACCTGACCCCGCTGTTTCCGACCGAACAGTTGAAGCTGGAACGCGACTTAAAGTCCGAAGAAAACCTGACCGGACGT
GCCATCGACCTGATTTCCCCTATCGGCAAAGGTCAGCGCGCCCTCTTGGTTGCCCCGCCCAAAAGCGGTAAAACCGTGAT
GCTGCAAAACATTGCCCACGCCGTTACCGCAAACTATCCCGAAGTCGAACTCATCGTCCTCTTGATTGACGAACGTCCCG
AAGAAGTAACCGAAATGAGCCGCTCCGTCCGTGGCGAAGTAGTCTCCTCCACCTTTGACGAGCCGGCTCAACGCCACGTC
CAAGTTGCCGAAATGGTGCTTGAAAAAGCCAAGCGTATGGTGGAACACAAAAAAGACGTGGTCATCCTGCTGGATTCGAT
TACCCGCCTTGCCCGCGCCTACAATACCGTCGTGCCTACCTCGGGCAAAATCCTGACCGGCGGTGTCGATGCCAACGCGC
TGCATCGTCCCAAACGTTTCTTCGGCGCGGCGCGCAACGTGGAAGAAGGCGGTTCGCTGACCATCATCGCCACCGCATTG
GTTGAAACCGGCAGCCGTATGGACGATGTGATTTACGAAGAATTCAAAGGCACCGGCAATATGGAATTGCACCTTGACCG
CCGTATGGCGGAAAAACGCCTCTTCCCCGCCATCAACATCAACAAATCCGGCACGCGCCGCGAAGAGCTGCTTGTCCCAA
ACGACCAGTTACAACGTATGTGGCTCTTACGCAAGTTCCTGCACCCGATGGACGAAATCGAGGCAGCCGAATTTTTAATC
GGGAAAATCAAAGCCTCTAAAAACAACGACGATTTCTTTGAACTGATGCGCGGCAAATAA

Domains

Predicted by InterproScan.

• Rho termination factor, RNA-binding domain (Rho_RNA_bind) (52-125)

• Rho termination factor, N-terminal domain (Rho_N) (5-47)

• ATP synthase alpha/beta family, nucleotide-binding domain (ATP-synt_ab) (160-363)

3D structure

• AlphaFold DB: Q9K0I3

References

• [1]: Yao-Hui Sun et al. (2005) Identification and characterization of genes required for competence in Neisseria meningitidis. Journal of Bacteriology 187(9):3273-6. [PMID: 15838056]