Detailed information    

experimental Experimentally validated

Overview

Name   ptsI   Type   Unclear
Locus tag   KZH42_RS00175 Genome accession   NZ_CP079941
Coordinates   34859..36634 (-) Length   591 a.a.
NCBI ID   WP_002225691.1    Uniprot ID   E6MZN1
Organism   Neisseria meningitidis strain C311     
Function   require for natural transformation   
Unclear

Function

ptsI mutant has a substantially reduced transformation frequency.


Genomic Context

Location: 29859..41634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH42_RS00155 (KZH42_00155) - 30449..31324 (+) 876 WP_002244347.1 NUDIX hydrolase family protein -
  KZH42_RS00160 (KZH42_00160) - 31333..32271 (+) 939 WP_002225688.1 ABC transporter ATP-binding protein -
  KZH42_RS00165 (KZH42_00165) - 32289..32432 (+) 144 WP_162467380.1 hypothetical protein -
  KZH42_RS00170 (KZH42_00170) - 33087..34058 (-) 972 Protein_28 IS5 family transposase -
  KZH42_RS00175 (KZH42_00175) ptsI 34859..36634 (-) 1776 WP_002225691.1 phosphoenolpyruvate--protein phosphotransferase Unclear
  KZH42_RS00180 (KZH42_00180) - 36634..36903 (-) 270 WP_002218178.1 HPr family phosphocarrier protein -
  KZH42_RS00185 (KZH42_00185) - 36972..37409 (-) 438 WP_002225692.1 PTS sugar transporter subunit IIA -
  KZH42_RS00190 (KZH42_00190) - 37491..38054 (-) 564 WP_002242054.1 hypoxanthine-guanine phosphoribosyltransferase -
  KZH42_RS00195 (KZH42_00195) - 38123..38947 (-) 825 WP_002225694.1 DNA ligase -
  KZH42_RS00200 (KZH42_00200) - 39039..39671 (+) 633 WP_002218182.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 591 a.a.        Molecular weight: 65143.76 Da        Isoelectric Point: 5.9263

>NTDB_id=1096 KZH42_RS00175 WP_002225691.1 34859..36634(-) (ptsI) [Neisseria meningitidis strain C311]
MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAERFDAAVKATRKELEQLRSAIPENAPTELGAFISLHL
MLLTDVTLSREPVDILREQKINAEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLITEGETVIVDGINGVLIIAPDESV
LNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIELVGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYE
VYSAIVKKMKGKSVTIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVHGPVRMMWP
MITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSILKLVDFISVGTNDLIQYILSVDRGDDSVSH
LYQPGHPAVLKMLQHVIRTANRMDKDVSVCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKV
IRCEDEEKSEKLIKQMNSVSVEEEADFKGRK

Nucleotide


Download         Length: 1776 bp        

>NTDB_id=1096 KZH42_RS00175 WP_002225691.1 34859..36634(-) (ptsI) [Neisseria meningitidis strain C311]
ATGAGTATCGTGCTGCACGGCGTGGCGGCGGGCAAAGGCATTGCCGTCGGTTGCGCCCACCTGATTGCGCGCGGTACGGA
GGAAGTGCCGCAGTATGATGTTGCGGAGGCGGACACCGATGCCGAAGCCGAACGTTTCGATGCCGCCGTCAAAGCCACGC
GCAAAGAGTTGGAACAGCTCCGCAGCGCGATTCCCGAAAACGCCCCGACCGAGTTGGGCGCGTTCATCTCGCTACACCTG
ATGCTCTTGACCGATGTTACCTTGTCGCGCGAACCCGTCGATATTTTAAGGGAACAAAAAATCAACGCCGAGTGGGCATT
GAAGCAGCAGAGCGACAAACTCGCCGCCCAATTCGACAATATGGACGATGCCTATTTGCGCGAACGCAAGCAGGATATGC
TGCAAGTCGTCCGCCGCATCCACAACAACCTGATCGGGCAGGGCAACGAGTTGGAAGTTGCCGACAACCTGTTTGACGAA
ACCGTTCTGATTGCAAACGACCTTTCGCCCGCCGACACGGTTTTGTTTAAAGAGCAGCGCATTGCCGCCTTCGTTACCGA
TGCCGGCGGCCCCACCGGGCATACGGCGATTTTGGGCAGGAGCTTGGACATCCCGTCCGTCGTCGGGCTGCACAACGCGC
GCAAACTGATTACCGAAGGCGAAACGGTCATTGTGGACGGTATCAACGGCGTGTTGATTATCGCGCCGGATGAGTCGGTG
TTGAACGAATACCGCCGCCGTGCCCGCGAATACCGCAGCCACAAACGCGATTTGAACAAGCTCAAAAAAACCGCCGCCGC
CACCGCCGACGGGGTCTGCATCGAGCTTGTGGGCAATATAGAATCCGCCGAAGACGTGAAACCGCTGCACAACCTCGGCG
CAGACGGCATCGGGCTGTTCCGCAGCGAGTTTCTTTACCTGAACCGCGATACGATGCCGTCTGAAGACGAGCAGTACGAA
GTGTACAGCGCGATTGTCAAAAAAATGAAAGGCAAAAGCGTAACGATACGGACAGTCGATTTAGGTGTGGACAAAAACCC
GCGCTGGTTCGGGAAAAACAGCACGCCCAACGGCAGCCTCAACCCCGCGCTGGGCATGACCGGCATCCGCCTGTGCCTTG
CCGAACCGGTCATGTTCCGCACCCAGATGCGCGCCATCCTCCGTGCGGCGGTACACGGCCCCGTGCGGATGATGTGGCCG
ATGATTACCTCCGTATCCGAAGTGCGCCAGTGCCTCATCCACCTCGACACCGCGCAACGCCAGCTTGCCGAACGCGGCGA
TGCCTTCGGTAAAGTCGGCATCGGCTGTATGATTGAAATTCCGTCTGCCGCGCTGACCGTCGGCAGTATTTTGAAACTGG
TCGATTTCATCTCCGTCGGTACCAACGACCTGATTCAATACATCTTGTCCGTCGATCGCGGCGACGACAGCGTCAGCCAC
CTCTACCAGCCCGGCCATCCCGCCGTGCTGAAAATGCTGCAACACGTCATCCGTACCGCCAACCGCATGGACAAAGACGT
ATCCGTATGCGGCGAGATGGCGGGCGATACCGCGTTTACCCGCGTTTTATTGGGTATGGGGCTGCGCCGTTTTTCCATGA
ACCCCAACAACATCCTGCCCGTCAAAAACATCATTCTGCACAGCAATGTCGGACAGCTCGAAAGCGATATTGTGAAAGTC
ATCCGCTGCGAAGACGAAGAGAAGTCGGAAAAGCTGATCAAACAGATGAACAGCGTGTCTGTCGAGGAAGAAGCCGACTT
CAAGGGGCGGAAATAA

Domains

Predicted by InterproScan.

PEP-utilising enzyme, N-terminal (PEP-utilisers_N)

(6-129)

PEP-utilising enzyme, mobile domain (PEP-utilizers)

(160-230)

PEP-utilising enzyme, PEP-binding domain (PEP-utilizers_C)

(257-548)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6MZN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Yao-Hui Sun et al. (2005) Identification and characterization of genes required for competence in Neisseria meningitidis. Journal of Bacteriology 187(9):3273-6. [PMID: 15838056]