Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACK25W_RS12030 Genome accession   NZ_CP179722
Coordinates   2364626..2366950 (-) Length   774 a.a.
NCBI ID   WP_106071787.1    Uniprot ID   -
Organism   Bacillus pumilus strain FJAT-56819     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2359626..2371950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK25W_RS12015 (ACK25W_12015) dtd 2361275..2361718 (-) 444 WP_060596866.1 D-aminoacyl-tRNA deacylase -
  ACK25W_RS12020 (ACK25W_12020) - 2361737..2363947 (-) 2211 WP_060596867.1 RelA/SpoT family protein -
  ACK25W_RS12025 (ACK25W_12025) - 2364090..2364602 (-) 513 WP_034661543.1 adenine phosphoribosyltransferase -
  ACK25W_RS12030 (ACK25W_12030) recJ 2364626..2366950 (-) 2325 WP_106071787.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACK25W_RS12035 (ACK25W_12035) - 2367032..2367385 (-) 354 WP_106072392.1 lipopolysaccharide assembly LapA domain-containing protein -
  ACK25W_RS12040 (ACK25W_12040) - 2367487..2367975 (-) 489 WP_066030599.1 cation:proton antiporter regulatory subunit -
  ACK25W_RS12045 (ACK25W_12045) secDF 2368163..2370391 (-) 2229 WP_060596871.1 protein translocase subunit SecDF -
  ACK25W_RS12050 (ACK25W_12050) - 2370433..2370729 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87478.20 Da        Isoelectric Point: 4.9974

>NTDB_id=1092862 ACK25W_RS12030 WP_106071787.1 2364626..2366950(-) (recJ) [Bacillus pumilus strain FJAT-56819]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPAQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSKRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDRFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMQN
PKPFIMIEDARLEEIRTIGANQNHIKMSLKDEDKLLDCVGFHQGQLKEEIVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQEVNIPIYHVHAENVSTFDVEGKYIVFADVPADEHLLRRLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKETIIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=1092862 ACK25W_RS12030 WP_106071787.1 2364626..2366950(-) (recJ) [Bacillus pumilus strain FJAT-56819]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGGATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGCG
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTCCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACGGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCGATTGTCCATCCTAAGCA
GCCGGGCTGTGAGTATCCCTTTAAAGAACTTGCCGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCACTTCTTGGGAAGC
TGCCAGCTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCCAAAAAAGGGCTTGTGCAGCTTAGGCAGTCGAAAAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCTACTCT
TGAAGAAGCAAATGAAGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGGCGAATTGAGCAGGCAGATC
CAGCTGTTCATCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAAGAACTCAATAAAGAA
CGTCAAAAAATTGTGAGTACGATCACAGAAGAAGCCATTCAAATGGTTGAAGAGACAGGTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATAGATTTTCACGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATCCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGTGACATTCTGCCGCATTTCGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGAGCGGATGATGT
TCCTTTACTAAGAGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTCG
ATGCCGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGCGAGGCTTGAAGAGATCCGAACGATTGGGGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCATCAAGGCCAATTAAAAGAAGAAATTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCTCAGCTTATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACAATCTCCACGCTTGATCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGAAGTCAATATCCCAATTTATCATGTCCATGCAGAAAATGTTT
CAACGTTCGATGTAGAGGGCAAGTATATCGTCTTTGCGGATGTTCCTGCAGATGAGCATTTGCTAAGGCGACTTTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCTTCTTTCCCTAGCCGTGATCA
ATTCAAATGGTTTTATGGATTCTTGTTAAAACGAGGAAGTTTCCCCCTCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGAAACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(309-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.568

100

0.678

Multiple sequence alignment