Detailed information    

experimental Experimentally validated

Overview

Name   nlaIV   Type   Auxiliary factor
Locus tag   NMB_RS05385 Genome accession   NC_003112
Coordinates   1046870..1047601 (-) Length   243 a.a.
NCBI ID   WP_002217109.1    Uniprot ID   Q9JZI8
Organism   Neisseria meningitidis MC58     
Function   inhibit foreign DNA acquisition   
Defense system

Function

In the wildtype, an inverse relationship between the transformation frequency and the number of NlaIV restriction sites in DNA was observed when the transforming DNA harboured a heterologous region for selection (ermC) but not when the transforming DNA was homologous with only a single nucleotide heterology.


Genomic Context

Location: 1041870..1052601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS05360 (NMB1028) - 1042235..1043110 (+) 876 WP_002219215.1 DMT family transporter -
  NMB_RS05365 (NMB1029) aspA 1043182..1044579 (-) 1398 WP_002217106.1 aspartate ammonia-lyase -
  NMB_RS05375 (NMB1030) - 1045040..1045603 (-) 564 WP_002217107.1 YceI family protein -
  NMB_RS05380 (NMB1031) leuB 1045766..1046836 (-) 1071 WP_002213431.1 3-isopropylmalate dehydrogenase -
  NMB_RS05385 (NMB1032) nlaIV 1046870..1047601 (-) 732 WP_002217109.1 NgoBV family restriction endonuclease Auxiliary factor
  NMB_RS05390 (NMB1033) - 1047617..1048888 (-) 1272 WP_002223735.1 DNA cytosine methyltransferase -
  NMB_RS05395 (NMB1034) leuD 1049063..1049704 (-) 642 WP_002222556.1 3-isopropylmalate dehydratase small subunit -
  NMB_RS05400 (NMB1035) - 1049766..1050020 (-) 255 WP_002213442.1 hypothetical protein -
  NMB_RS05405 (NMB1036) leuC 1050117..1051526 (-) 1410 WP_002219221.1 3-isopropylmalate dehydratase large subunit -

Sequence

Protein


Download         Length: 243 a.a.        Molecular weight: 28811.32 Da        Isoelectric Point: 9.6318

>NTDB_id=1091 NMB_RS05385 WP_002217109.1 1046870..1047601(-) (nlaIV) [Neisseria meningitidis MC58]
MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVGNIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKD
RSRELLEVKAFNRNASPGFDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEEAYKKHYNRSISIPRWHEIAHKY
KKK

Nucleotide


Download         Length: 732 bp        

>NTDB_id=1091 NMB_RS05385 WP_002217109.1 1046870..1047601(-) (nlaIV) [Neisseria meningitidis MC58]
ATGGTAAAACTTACTGCACAACAAATATTTGATAAGTTGCTGAATGAGGAAAAAATCTTATCGGTCAATGGTCAAATTAG
ATTTTTCTTGGGCGATGTGGATATTATCGTCAAACAAAAAGATGTCGTCGGCAACATCATTCAGGAATGGCTCGGCGGAT
GGTTGAGAAAAAGGGAAATTGAATTTGATGTTTCAACCAATACCCAAATGCCCCCCGATTTCTTTTTAAATAAAAAAGAC
CGCAGTAGAGAATTGTTGGAAGTAAAGGCGTTCAACCGAAATGCCAGTCCGGGTTTTGATATTGCAGATTTTAAAATGTA
TTCTGATGAAATCATTCATAAACCCTATATGCTGGATGTAGACTATTTAATATTCGGTTACGATATGGACGACAACGGCA
ATGTAACCATCAAGGATTTATGGCTTAAAAAAGTATGGCAAATTACGAGAAGCATGGATGGGTGGGCAATTAATCTTCAA
GTCAAAAAAGGCGTGGTGCATAAAATCCGCCCGGGTGTTTGGTACAGCATAAATAAAAAGAATATGCCTATGTTTGAATG
CTTGGAAGATTTTGTTTCCGCAATTGAAGAAACCGTTTATCAAAACCCGGCAACACGGCATAACGCATCCTTATGGAAAA
AGAAATTTGAGGAAGCCTATAAAAAACACTACAACCGATCGATTTCCATACCTCGTTGGCACGAAATTGCACACAAATAC
AAAAAGAAATAA

Domains

Predicted by InterproScan.

NgoBV restriction endonuclease (RE_NgoBV)

(3-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9JZI8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Ole Herman Ambur et al. (2012) Restriction and sequence alterations affect DNA uptake sequence-dependent transformation in Neisseria meningitidis. PloS One 7(7):e39742. [PMID: 22768309]