Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACKUFW_RS12770 Genome accession   NZ_CP178610
Coordinates   2613738..2616098 (-) Length   786 a.a.
NCBI ID   WP_202735645.1    Uniprot ID   -
Organism   Bacillus velezensis strain GUMHT p116     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2608738..2621098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUFW_RS12750 (ACKUFW_12750) - 2608814..2610376 (+) 1563 WP_071391557.1 SH3 domain-containing protein -
  ACKUFW_RS12755 (ACKUFW_12755) dtd 2610402..2610845 (-) 444 WP_007408194.1 D-aminoacyl-tRNA deacylase -
  ACKUFW_RS12760 (ACKUFW_12760) relA 2610858..2613062 (-) 2205 WP_202735644.1 GTP diphosphokinase -
  ACKUFW_RS12765 (ACKUFW_12765) - 2613220..2613732 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  ACKUFW_RS12770 (ACKUFW_12770) recJ 2613738..2616098 (-) 2361 WP_202735645.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACKUFW_RS12775 (ACKUFW_12775) - 2616154..2616480 (-) 327 WP_003152709.1 lipopolysaccharide assembly LapA domain-containing protein -
  ACKUFW_RS12780 (ACKUFW_12780) - 2616544..2617041 (-) 498 WP_012118088.1 cation:proton antiporter regulatory subunit -
  ACKUFW_RS12785 (ACKUFW_12785) secDF 2617173..2619392 (-) 2220 WP_202735646.1 protein translocase subunit SecDF -
  ACKUFW_RS12790 (ACKUFW_12790) - 2619429..2619725 (-) 297 WP_007408189.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87462.90 Da        Isoelectric Point: 4.9227

>NTDB_id=1090491 ACKUFW_RS12770 WP_202735645.1 2613738..2616098(-) (recJ) [Bacillus velezensis strain GUMHT p116]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSQG
EPIMIYGDYDADGVTSTSVMLTALRQLSADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGMERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVTKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCVGFNKGELEAGVVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLICFNEKNAQKLREKELSGELAEITSESEAEALQLDGKYAVFLDVPPSADR
LQRLLDGKKPERIYFVFINHEEHFLSSFPSRSDFKWFYAFLLKRGNFDLSKHGSELARHKGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=1090491 ACKUFW_RS12770 WP_202735645.1 2613738..2616098(-) (recJ) [Bacillus velezensis strain GUMHT p116]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAACTGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCAGTGATGCTGACGGCACTCAGACAGCT
GTCGGCGGACGTTGACTTTTATATTCCCGACCGTTTTACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGCTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTCGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGATGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAGCTATGCTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCTCTTCATGATGAAAACAGATTAATT
GCGTCACTCGGAATGGAAAGGCTTCGGACGACAAATCGGCCGGGCCTCAAAGCTCTGATTAAGCTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATCGGATTTCAGGTCGCACCGAGGCTCAATGCCGTCGGAAGAATAGAACAGGCCGATC
CCGCCGTTCACCTGCTGATGACCGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGACAAAAGCCGGCTGGAATCCGGGAGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGGCCCGCTG
TTGTGCTTGGAATAGATGAGGAGCAGGGAATCGCTAAAGGGTCGGCGCGAAGCATCAGAGGCTTCAACCTTTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCCGGAATGACGTTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTTACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTTGAAGACATAACCGTTGAAAGCATTACAGAATTGAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTGCTTGATGATGTGCGCCTGATCGGAGCGAATAAAAACCACGTAAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCGTCGGCTTTAACAAAGGAGAGCTGGAAGCCGGAGTTGTTCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAGCCCCAGCTGATGATTAAAGATGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCCGGGGCAAGCGGACGTGGGAAGATACGGTCACCGCGCTGCCGAAGGAAAA
ACGGGTGCTCATCTGCTTTAACGAAAAAAACGCTCAGAAGCTCCGTGAAAAAGAGCTGTCGGGCGAGCTTGCCGAGATCA
CTTCGGAATCCGAGGCGGAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCGTTTGCTTGACGGGAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTTCTGTCTTC
ATTTCCATCGCGGAGTGATTTCAAGTGGTTTTACGCATTTCTCTTGAAAAGGGGCAATTTTGACTTAAGTAAGCATGGTT
CGGAGCTTGCGAGACACAAAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGCGCGAAAAAACGCGACCTTGCAGATTCGCAAACCTACCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCGGCTCATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAGGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(315-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

81.934

100

0.819

Multiple sequence alignment