Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   ABWW24_RS04255 Genome accession   NZ_AP031330
Coordinates   761944..762645 (-) Length   233 a.a.
NCBI ID   WP_014629427.1    Uniprot ID   H9ZQX5
Organism   Thermus thermophilus strain TF25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 756944..767645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWW24_RS04220 (TthTF25_08260) - 757200..757757 (+) 558 WP_124104344.1 Uma2 family endonuclease -
  ABWW24_RS04225 (TthTF25_08270) - 757791..758153 (-) 363 WP_060384345.1 HepT-like ribonuclease domain-containing protein -
  ABWW24_RS04230 (TthTF25_08280) - 758140..758439 (-) 300 WP_008631315.1 nucleotidyltransferase -
  ABWW24_RS04235 (TthTF25_08290) - 758462..759004 (-) 543 WP_353300788.1 Uma2 family endonuclease -
  ABWW24_RS04240 (TthTF25_08300) - 759125..759430 (-) 306 WP_353300789.1 DUF1640 domain-containing protein -
  ABWW24_RS04245 (TthTF25_08310) - 759679..760029 (-) 351 WP_353300790.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABWW24_RS04250 (TthTF25_08320) comZ 760260..761933 (-) 1674 WP_353300791.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  ABWW24_RS04255 (TthTF25_08330) pilA/pilA3 761944..762645 (-) 702 WP_014629427.1 PilW family protein Machinery gene
  ABWW24_RS04260 (TthTF25_08340) pilA/pilA2 762642..763223 (-) 582 WP_353300792.1 type II secretion system protein Machinery gene
  ABWW24_RS04265 (TthTF25_08350) pilA/pilA1 763258..763728 (-) 471 WP_165739610.1 GspH/FimT family protein Machinery gene
  ABWW24_RS04270 (TthTF25_08360) - 763894..764874 (+) 981 WP_353300793.1 YpdA family putative bacillithiol disulfide reductase -
  ABWW24_RS04275 (TthTF25_08370) ilvB 765030..766718 (+) 1689 WP_353300794.1 biosynthetic-type acetolactate synthase large subunit -
  ABWW24_RS04280 (TthTF25_08380) ilvN 766715..767227 (+) 513 WP_353300795.1 acetolactate synthase small subunit -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24920.35 Da        Isoelectric Point: 7.4626

>NTDB_id=108758 ABWW24_RS04255 WP_014629427.1 761944..762645(-) (pilA/pilA3) [Thermus thermophilus strain TF25]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGNQNRAFSLPL
PPLSGSDGGPKDTLTLYYVTSLRDLASACRRVDYGFDGDTLRRSDLNATPSSGSDCTTPSSNLQPLAEGMLALDIQYQCS
DGSQKDTPDCGTDAYPRSAKVTVAGYSLTPVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=108758 ABWW24_RS04255 WP_014629427.1 761944..762645(-) (pilA/pilA3) [Thermus thermophilus strain TF25]
ATGAAGCGCGGGTTCACCCTGGTGGAAGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACAAGCGAGCTCGCCCGGAACACCCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGGGCCCGCTACTGGAATAGCGGCAACCAAAACCGGGCCTTCAGCCTCCCCCTT
CCCCCTCTTTCGGGAAGCGACGGAGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGACGGAGACACCCTACGCCGGAGCGACCTGAACGCCACCCCCTCTTCGGGAA
GCGATTGCACCACCCCCTCTTCGAACTTGCAGCCCTTGGCCGAAGGGATGTTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGAAGCCAGAAGGACACCCCCGACTGCGGTACAGACGCCTACCCCCGCTCGGCCAAGGTCACGGTGGCGGGCTACTC
CCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H9ZQX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

95.708

100

0.957

Multiple sequence alignment