Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACK2WG_RS13750 Genome accession   NZ_CP176536
Coordinates   2676820..2679180 (-) Length   786 a.a.
NCBI ID   WP_409509056.1    Uniprot ID   -
Organism   Bacillus spizizenii strain 29-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2671820..2684180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2WG_RS13730 (ACK2WG_13730) - 2671896..2673449 (+) 1554 WP_409509055.1 SH3 domain-containing protein -
  ACK2WG_RS13735 (ACK2WG_13735) dtd 2673476..2673916 (-) 441 WP_014114616.1 D-aminoacyl-tRNA deacylase -
  ACK2WG_RS13740 (ACK2WG_13740) relA 2673929..2676133 (-) 2205 WP_014114617.1 GTP diphosphokinase -
  ACK2WG_RS13745 (ACK2WG_13745) - 2676302..2676814 (-) 513 WP_014114618.1 adenine phosphoribosyltransferase -
  ACK2WG_RS13750 (ACK2WG_13750) recJ 2676820..2679180 (-) 2361 WP_409509056.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACK2WG_RS13755 (ACK2WG_13755) - 2679247..2679579 (-) 333 WP_079288037.1 lipopolysaccharide assembly LapA domain-containing protein -
  ACK2WG_RS13760 (ACK2WG_13760) - 2679657..2680154 (-) 498 WP_061186676.1 cation:proton antiporter regulatory subunit -
  ACK2WG_RS13765 (ACK2WG_13765) secDF 2680301..2682514 (-) 2214 WP_014114622.1 protein translocase subunit SecDF -
  ACK2WG_RS13770 (ACK2WG_13770) comN 2682553..2682849 (-) 297 WP_003222684.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88075.64 Da        Isoelectric Point: 5.0072

>NTDB_id=1080796 ACK2WG_RS13750 WP_409509056.1 2676820..2679180(-) (recJ) [Bacillus spizizenii strain 29-4]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EQIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPGELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELVKLSGGNIGEANEETVGFQIAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLNAEDVPDLRNRLNEIADNTLTEEDFIPVQEVDLVCGVEDITVESITEMNLLSPFGMLN
PKPHVLVENAVLEDVRKIGANKNHVKMTVRNESSQLDCVGFNKGELEEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRMWEDTVSVLPSAKRAIVSFKEDSTTLLQAEDLRREVHVISSEEQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKTPERIYFIFLNQEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELARHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGVKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=1080796 ACK2WG_RS13750 WP_409509056.1 2676820..2679180(-) (recJ) [Bacillus spizizenii strain 29-4]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTCGAAATGAAGGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAACAGATTATGATATATGGCGATTATGACGCCGATGGAGTTACCAGTACGTCAGTGATGCTTCACACGCTGCAAAAGCT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGCTTTAAAGAAGGATATGGCCCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTCTGATTATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGGCTGGATGTCATTATTACCGATCACCATGAGCCGGGACCGGAGCTTCCGGATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACCTACCCGTTTAAAGAACTTGCCGGGGTCGGAGTTGCGTTTAAGCTTGCCCACGCTTTATTGGGCGAAC
TCCCGGGTGAACTGCTAGACTTGGCGGCAATCGGCACCATTGCCGATCTTGTCCCGTTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGTCGGACGAATCGGCTGGGCTTAAAAGAGCTGGTCAAGCTGTCAGGCGGAAACAT
CGGAGAAGCGAACGAAGAAACAGTCGGGTTCCAGATAGCACCAAGGCTGAATGCCGTCGGACGAATCGAACAGGCGGATC
CCGCTGTTCATTTGCTCATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCTGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGTCTCGATCAAACTGCAATCGT
CGTGGCCAAAGCCGGGTGGAATCCAGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTATCGTCCGGCCA
TTGTGCTTGGCATTGATGAAGAAAAAGGGATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGCTTTAATTTATTCGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGTTGAATGCCGAAGATGT
GCCGGATTTGCGAAACAGGCTGAATGAAATAGCAGACAATACGCTCACGGAAGAGGATTTTATTCCTGTACAGGAAGTCG
ATCTCGTCTGCGGTGTCGAGGACATTACGGTTGAAAGCATCACAGAAATGAACCTGCTGTCCCCATTTGGCATGCTGAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCTGTGCTGGAAGATGTCCGCAAGATCGGCGCTAATAAAAATCATGTGAAAAT
GACGGTCAGAAACGAGTCGTCTCAGCTTGATTGCGTCGGTTTTAATAAAGGTGAGCTCGAAGAAGGCATCGTCCCGGGTT
CAAGAATTTCAATTGTCGGTGAGATGTCTATCAACGAATGGAACAATAGAAAAAAACCGCAGTTAATGATTAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGATGTGGGAAGACACAGTTTCCGTCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACACTTCTTCAGGCTGAAGATCTTCGCCGTGAAGTGCATGTCATCA
GCTCTGAAGAACAAGCGAAGGCGTTTGACCTTGATGGTGCATATATTGTATTGCTGGATCCGCCGCCTTCGTTGGACATG
CTCGCACGTTTGCTGGAAGGCAAGACGCCTGAGCGGATATATTTCATTTTTCTCAATCAAGAGGACCACTTTTTATCAAC
ATTTCCCGCAAGAGACCATTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTAAAAAAACACGGAT
CAGAGCTTGCAAGACATAAAGGCTGGTCTGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTAGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTGGTTAGCGGAGTAAAGAAACGCGACCTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHHA1 domain (DHHA1)

(315-437)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

97.201

100

0.972

Multiple sequence alignment