Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ACKW6F_RS05320 Genome accession   NZ_CP176527
Coordinates   1087646..1091155 (+) Length   1169 a.a.
NCBI ID   WP_149125792.1    Uniprot ID   -
Organism   Bacillus safensis strain D-62     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1082646..1096155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKW6F_RS05290 (ACKW6F_05290) lepB 1083163..1083675 (+) 513 WP_149125793.1 signal peptidase I -
  ACKW6F_RS05295 (ACKW6F_05295) - 1083714..1084859 (-) 1146 WP_149126690.1 AbrB family transcriptional regulator -
  ACKW6F_RS05300 (ACKW6F_05300) - 1085126..1085548 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  ACKW6F_RS05305 (ACKW6F_05305) - 1085541..1086380 (+) 840 WP_024425496.1 M56 family metallopeptidase -
  ACKW6F_RS05310 (ACKW6F_05310) - 1086438..1086848 (+) 411 WP_024425497.1 DoxX family protein -
  ACKW6F_RS05315 (ACKW6F_05315) - 1087047..1087493 (-) 447 WP_024425498.1 ferritin-like domain-containing protein -
  ACKW6F_RS05320 (ACKW6F_05320) addB 1087646..1091155 (+) 3510 WP_149125792.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACKW6F_RS05325 (ACKW6F_05325) addA 1091133..1094837 (+) 3705 WP_149125791.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACKW6F_RS05330 (ACKW6F_05330) sbcD 1094864..1096039 (+) 1176 WP_149125790.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134959.15 Da        Isoelectric Point: 5.4870

>NTDB_id=1080661 ACKW6F_RS05320 WP_149125792.1 1087646..1091155(+) (addB) [Bacillus safensis strain D-62]
MEIQLLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLAKECGAAISEHILERNKRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSMDVIKGNWRY
EAVFRCVKTEFLFPLELARNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYIFLEEIQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHEHALYVLDPEQLEDKEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFSNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=1080661 ACKW6F_RS05320 WP_149125792.1 1087646..1091155(+) (addB) [Bacillus safensis strain D-62]
GTGGAGATTCAGTTATTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGTTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACACTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCACAGCAGCTCCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACGGCTGCTTTCACAGTGGATCGTTCC
TATCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCCAA
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGAGAAATAAACGTCATCTTCATACGCCGGATCTTGCTTATTTAG
AACGGCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTCACTCCTCATCTCACAGTATCTAAAAGTGCGAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCACTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCACCCGCTCATTGAATTGATTCGTTCGAGCATGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGATGCGTGAAGACAGAGTTCTTATTTCCGCTCGAACTTGCCAGAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGCTCTCGTTTCCATTACAGGCGGT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTGAATGATGTAAAGGAATGG
ATCGCACCTCCGCTTTTCCAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTGGAGGCAGTCTATAT
CTTTTTAGAGGAAATACAAGTGCCGGAAAAACTTGAAAAGGCGAGGCTGGAGGCTGAGGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTAATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGACTGGCATCTCTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCGAGAATGTATCAAGTGAAGTGCATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTATTGTCTGAGGATGACAGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTTGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGCTGTCGAGTCCATCCCATCATTTGTATCT
GTCTTATTCAGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATGAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGAGGACAAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAACCAATATCCCATCAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTGTCAAAAAACATCTTGTCGAGTTTGTTTTTTTCAAATCGAGCGAAAC
GCTTAAAACCAAACGTCACAAAAGAACTTTATGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCCTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
ATGACTGTGTCAACTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATCCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTCGGGCTTGAGTTAGGCTTTGGCGGCCAAGGACCACTTCCACCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATACTATGGGCTGGCCTTGCAAATGCTCACATACTTAGA
TCTGACCATTACGTATTCGAAGGAGTGGCTCGGTGTAGAAGCAACACCAGCTGGCATTTTATACTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAACAGGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAAGATGGGTCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTCCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCTGGCGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.719

99.316

0.653

Multiple sequence alignment