Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACKWCM_RS12705 Genome accession   NZ_CP176416
Coordinates   2494720..2497044 (-) Length   774 a.a.
NCBI ID   WP_197172640.1    Uniprot ID   -
Organism   Bacillus safensis strain GUCCb3-7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2489720..2502044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWCM_RS12690 (ACKWCM_12690) dtd 2491369..2491812 (-) 444 WP_034622406.1 D-aminoacyl-tRNA deacylase -
  ACKWCM_RS12695 (ACKWCM_12695) - 2491831..2494041 (-) 2211 WP_024427858.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  ACKWCM_RS12700 (ACKWCM_12700) - 2494184..2494696 (-) 513 WP_024423133.1 adenine phosphoribosyltransferase -
  ACKWCM_RS12705 (ACKWCM_12705) recJ 2494720..2497044 (-) 2325 WP_197172640.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACKWCM_RS12710 (ACKWCM_12710) - 2497126..2497476 (-) 351 WP_024423135.1 lipopolysaccharide assembly LapA domain-containing protein -
  ACKWCM_RS12715 (ACKWCM_12715) - 2497578..2498066 (-) 489 WP_024423136.1 cation:proton antiporter regulatory subunit -
  ACKWCM_RS12720 (ACKWCM_12720) secDF 2498254..2500482 (-) 2229 WP_024423137.1 protein translocase subunit SecDF -
  ACKWCM_RS12725 (ACKWCM_12725) - 2500524..2500820 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 86994.55 Da        Isoelectric Point: 4.8856

>NTDB_id=1079980 ACKWCM_RS12705 WP_197172640.1 2494720..2497044(-) (recJ) [Bacillus safensis strain GUCCb3-7]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGELPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEASEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVSLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVQAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDTLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDLPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=1079980 ACKWCM_RS12705 WP_197172640.1 2494720..2497044(-) (recJ) [Bacillus safensis strain GUCCb3-7]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGACCTAATGAACAGGCTTTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTGGCCATTGTCCATCCTAAGCA
GCCTGGCTGTGAATATCCCTTTAAAGAACTTGCTGGTGTAGGTGTCGCGTTTAAAGTGGCGCATGCACTTCTTGGAGAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTCGTACCGCTTCACGATGAGAATAGATGGCTC
GCCAAAAAAGGCCTTATGCAGCTTCGGCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTTT
AGAAGAAGCGAGCGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGGCGAATTGAACAGGCAGATC
CAGCCGTTCATCTTCTAATGACTGAGGATAAGGATGAAGCGGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCAGGTGATGACCAGTCTGCGATTGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
ATCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATTCAGCGGGTCG
ATGCCGTGTGCAAGGTAGACGAATTGACGGTTCAAGCGATTGAAGAAGCGGGGATGCTGTCACCATTTGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACACACTGCTTGACTGTGTTGGCTTTCACCAAGGGAAATTAAAAGAGGAAATTGTTCCAGGAA
GCCGCATTTCTGTGATTGGAGAAATGTCTGTCAATGAATGGAATAACCGGAAGAAGCCCCAGCTGATGTTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACACTTGATTCAGAAAA
ATCATTGGTTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATCTCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACATTTGATGTACAAGGAAAGTATGTGGTCTTTGCAGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCGAGAATTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCGTCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(311-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.439

100

0.677

Multiple sequence alignment