Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACKUBV_RS13240 Genome accession   NZ_CP176357
Coordinates   2797898..2798914 (+) Length   338 a.a.
NCBI ID   WP_031821574.1    Uniprot ID   A0AA47L644
Organism   Vibrio parahaemolyticus strain AG1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2792898..2803914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUBV_RS13225 (ACKUBV_13225) - 2793322..2794050 (-) 729 WP_015297397.1 redoxin family protein -
  ACKUBV_RS13230 (ACKUBV_13230) oxyR 2794221..2795129 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  ACKUBV_RS13235 (ACKUBV_13235) - 2795185..2797755 (-) 2571 WP_069535787.1 penicillin-binding protein 1A -
  ACKUBV_RS13240 (ACKUBV_13240) pilM 2797898..2798914 (+) 1017 WP_031821574.1 type IV pilus biogenesis protein PilM Machinery gene
  ACKUBV_RS13245 (ACKUBV_13245) pilN 2798898..2799476 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  ACKUBV_RS13250 (ACKUBV_13250) pilO 2799469..2800062 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACKUBV_RS13255 (ACKUBV_13255) pilP 2800052..2800567 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  ACKUBV_RS13260 (ACKUBV_13260) pilQ 2800599..2802347 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  ACKUBV_RS13265 (ACKUBV_13265) aroK 2802536..2803054 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37720.16 Da        Isoelectric Point: 6.6631

>NTDB_id=1079717 ACKUBV_RS13240 WP_031821574.1 2797898..2798914(+) (pilM) [Vibrio parahaemolyticus strain AG1]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWNLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1079717 ACKUBV_RS13240 WP_031821574.1 2797898..2798914(+) (pilM) [Vibrio parahaemolyticus strain AG1]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCAGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAATCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGAACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.32

100

0.843
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment