Detailed information NTG1078157

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: ACKFA7_RS12490
• Genome accession: NZ_CP175553
• Coordinates: 2614999..2617377 (-)
• Length: 792 a.a.
• NCBI ID: WP_007408204.1
• Uniprot ID: A7Z743
• Organism: Bacillus amyloliquefaciens strain 4-9-2
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2609999..2622377

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKFA7_RS12455 (ACKFA7_12455)	-	2611071..2611799 (+)	729	WP_038459338.1	amino acid ABC transporter ATP-binding protein	-
ACKFA7_RS12460 (ACKFA7_12460)	-	2611820..2612647 (+)	828	WP_007408208.1	transporter substrate-binding domain-containing protein	-
ACKFA7_RS12465 (ACKFA7_12465)	-	2612704..2613354 (+)	651	WP_038459340.1	amino acid ABC transporter permease	-
ACKFA7_RS12470 (ACKFA7_12470)	-	2613373..2614029 (+)	657	WP_015417903.1	amino acid ABC transporter permease	-
ACKFA7_RS12475 (ACKFA7_12475)	-	2614065..2614196 (-)	132	WP_003152741.1	YrzQ family protein	-
ACKFA7_RS12480 (ACKFA7_12480)	-	2614218..2614409 (-)	192	WP_003152738.1	hypothetical protein	-
ACKFA7_RS12485 (ACKFA7_12485)	-	2614422..2614880 (-)	459	WP_025649674.1	PRC-barrel domain-containing protein	-
ACKFA7_RS12490 (ACKFA7_12490)	recD2	2614999..2617377 (-)	2379	WP_007408204.1	ATP-dependent RecD-like DNA helicase	Machinery gene
ACKFA7_RS12495 (ACKFA7_12495)	-	2617396..2618016 (-)	621	WP_088461189.1	lipopolysaccharide assembly protein LapB	-
ACKFA7_RS12500 (ACKFA7_12500)	mnmA	2618104..2619219 (-)	1116	WP_038459345.1	tRNA 2-thiouridine(34) synthase MnmA	-
ACKFA7_RS12505 (ACKFA7_12505)	-	2619256..2620395 (-)	1140	WP_038459347.1	cysteine desulfurase family protein	-
ACKFA7_RS12510 (ACKFA7_12510)	cymR	2620414..2620830 (-)	417	WP_003152730.1	cysteine metabolism transcriptional regulator CymR	-
ACKFA7_RS12515 (ACKFA7_12515)	-	2621025..2622293 (+)	1269	WP_038459350.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88364.04 Da
• Isoelectric Point: 4.9490

>NTDB_id=1078157 ACKFA7_RS12490 WP_007408204.1 2614999..2617377(-) (recD2) [Bacillus amyloliquefaciens strain 4-9-2]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDEAFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQIKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=1078157 ACKFA7_RS12490 WP_007408204.1 2614999..2617377(-) (recD2) [Bacillus amyloliquefaciens strain 4-9-2]
GTGGATCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAAGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTACGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGAATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGAGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCAATGAAAATATATCAGGCCTACGAAACGGAAACCCTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAA
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
CACGGAAATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATTGAAGGAGAGCGCTGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAGACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGAGGACCTGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGGCGTTTCCGGTG
CTGCTTGCCGCGCCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGTCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTACAATGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTAGCTTCTGAGGTGCTGCCGACCGTGCGCCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCAAAGG
GGCTGACCGCGCCGACAAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATTAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCCAGGGATATTCAAGTGCTTGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGAAGCGAATTCCCGATTGTCG
TCCTGCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGAGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCGTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• AAA domain (AAA_30) (341-548)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

3D structure

• AlphaFold DB: A7Z743

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.875	99.874	0.888