Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ACIU4M_RS15125 Genome accession   NZ_CP174375
Coordinates   2907161..2910670 (+) Length   1169 a.a.
NCBI ID   WP_407708325.1    Uniprot ID   -
Organism   Bacillus altitudinis strain SF5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2902161..2915670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIU4M_RS15095 (ACIU4M_15095) lepB 2902464..2902976 (+) 513 WP_007499139.1 signal peptidase I -
  ACIU4M_RS15100 (ACIU4M_15100) - 2903216..2904358 (-) 1143 WP_007499137.1 AbrB family transcriptional regulator -
  ACIU4M_RS15105 (ACIU4M_15105) - 2904634..2905056 (+) 423 WP_007499135.1 BlaI/MecI/CopY family transcriptional regulator -
  ACIU4M_RS15110 (ACIU4M_15110) - 2905049..2905888 (+) 840 WP_025207106.1 M56 family metallopeptidase -
  ACIU4M_RS15115 (ACIU4M_15115) - 2905946..2906356 (+) 411 WP_008348626.1 DoxX family protein -
  ACIU4M_RS15120 (ACIU4M_15120) - 2906548..2906994 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  ACIU4M_RS15125 (ACIU4M_15125) addB 2907161..2910670 (+) 3510 WP_407708325.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACIU4M_RS15130 (ACIU4M_15130) addA 2910648..2914352 (+) 3705 WP_407708326.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACIU4M_RS15135 (ACIU4M_15135) sbcD 2914378..2915553 (+) 1176 WP_138278368.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134459.33 Da        Isoelectric Point: 5.1018

>NTDB_id=1076989 ACIU4M_RS15125 WP_407708325.1 2907161..2910670(+) (addB) [Bacillus altitudinis strain SF5]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMMPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPLSEELRGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDTQPNELDLFRMTGKTYSQLYQLAKECGAAISENILERNKRHLHTPDLAYLENQYEQRPLQPYQEETPHLTVSKSANK
RAEIEGVARDILDLVRGEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIEFIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLFQLQKRLKKAQKLRDMVEAVYVFLEEAQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLSSLKFALIPPSLDQVFIGSMDLSRMYQVKCTFVIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSLYLYLSYSASDTEGRSLLPSTLIKYCEELMPNHQQALYVLDPEQLDDQEQLKFVTNEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNHLMTSSNSDVSKKIMSSLFFTNRATRLNPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKLISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMEATPAGILYFHIHDPLIQAPIPLGEDAIEQEILKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFEEAGERITSGEVEINPYKLKDQTP
CSFCSFKSICQFDESIEDNEFRVLTSEKDDVMLDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=1076989 ACIU4M_RS15125 WP_407708325.1 2907161..2910670(+) (addB) [Bacillus altitudinis strain SF5]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATTCTAGAAGAAATCAAAGAACAACTGCG
GCTTGATCCGCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGTGGAATGATCAGGGCCAAAGTCTTTAGTTTTACTCGGCTTGCCTGGTCTATATTACAGCAGACTGGC
GGTGCGAACCGTCAATTTGTCACAAGCACAGGAATTCAAATGCTCTTAAGAAAAGTAATAGAAGAACAGAAAGACAAGTT
TAAGGTATTCAAAAAGGCGAGTGATAAACCGGGATTTGTTGAGCAAATAGAAAAGACGATGGCTGAATTCAAAAGGTACT
GTATGATGCCGGAGGATATTGAAAAAATCTCGGCATCCAGCATGCATTCAGAGTATACAGAAGAAAGACGTGCGGCGGAA
AAATTGCATGATCTTCATGTTCTTTATCAGCAGATGGAGCAGCATCTGCAAGATGAGTATGTGCACTCAGAGGATTACTT
GACACTTCTGGCTCAGCAAATTCCTTTATCAGAAGAATTGAGAGGAGCACACATTTATATAGATGGTTTTTATCAGTTTA
CCCCCCAGCAGCTTCTTGTCATTGAACAGCTTTTGCTACATGCAGAAAAAATAACAGCTGCATTCACAGTGGATCGTTCC
TATCATGACACGCAGCCAAATGAACTTGATTTATTTCGCATGACAGGTAAGACATATTCCCAGCTTTATCAGTTGGCTAA
AGAGTGCGGAGCGGCTATTTCTGAGAACATTTTGGAGAGAAATAAGCGGCATCTTCATACCCCAGATCTAGCTTATTTAG
AAAATCAATATGAACAGAGACCTTTGCAGCCGTATCAAGAAGAAACGCCTCACCTTACCGTATCGAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTTGATTTAGTAAGAGGGGAAGGTCTTAGGTTACGAGACATCTCAAT
TGTTGCAAGACATGTAGACGACTATAAAGATACGTTAAAAGAGGTGTTTCGAGATTACGATATTCCTTTCTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAGTTCATTCGTTCGAGCCTCGATGTCATAAAAGGGAACTGGCGGTAT
GAGGCGGTGTTTCGCTGTGTGAAAACGGAGTTCTTATTTCCGCTTGAACTCGCTAAAAACAAAGCAAGAGAACAGGCGGA
TCAGCTTGAAAACTATTGTATTGCTTACGGTGTAAAAGGTGAACGCTGGACGAATGGTTCACGATTTCATTATCGCCGCT
TCCAATCACTAGATGAAGATTTTGGACAAACCGATCAAGAAATAGAAATGGAGCAAATGCTGAATGATGTAAAAGAATGG
ATTGTTCCGCCTCTCTTTCAATTGCAAAAAAGATTGAAAAAAGCGCAAAAGCTAAGAGACATGGTGGAGGCAGTGTATGT
CTTTCTAGAAGAAGCCCAAGTGCCAGAAAAGCTTGAAAAGGCAAGGCTTGAGGCAGAAGAAGCAGGCAGGTTAGCTGAAG
CGATGCAGCATGGGCAAGTGTGGGATGCTGTCATTCAATTGATGGATGAATTTGTTGAAATGCTGGGCGAGGAGGAGCTC
TCATTTCCATTATTTCAACAGATGATGGATACAGGACTGTCCTCTCTCAAGTTTGCTTTGATTCCCCCTTCACTTGATCA
AGTGTTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTAAAGTGTACGTTTGTCATTGGAGTAAATGATGGAGTTA
TACCTGCACGGCCCTCTGATGAAAGTGTATTGTCTGAAGACGACCGGGAATGGTTAAAACGGGCAGGAGCAGAGCTGGCA
GAGACAGGGAAGGAACGACTGCTGGATGAACAATTTTTGATATATCAAGCGCTGTCCAGTCCATCGCTCTACCTGTATTT
ATCCTATTCAGCTTCAGATACGGAAGGGAGGTCTCTTTTACCTTCGACCCTCATCAAGTATTGTGAGGAACTGATGCCAA
ACCATCAGCAAGCCCTCTATGTGTTAGATCCAGAGCAGTTGGATGATCAGGAGCAATTAAAATTTGTAACAAACGAGCAT
GTCTCATTATCCTATACCGTTTCGCAGCTGCAACAATGGCTCAATCAATATCCGATCAGCGGCGTATGGTGGAGTGTATA
TAATCATTTGATGACCTCGTCAAACAGCGACGTATCAAAAAAAATCATGTCCAGCTTGTTCTTTACAAATAGAGCGACAC
GTTTAAATCCGAATGTCACAAAAGAACTTTACGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCA
TGTGCATTTTCTCATTTTGCTTCCCACGGGCTAAAACTAAAAGACCGACAATTTTACAAATTAGAAGCACCTGATATCGG
CCAGCTCTTTCACTCGGCTTTAAAACTGATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
AAGATTGTGTGACCTACTCAAGGCATGCAATTGAACAGCTGGCTCCACGTCTGCAAAAAGAAATTTTATTAAGCTCTAAT
CGGCATGCTTATATAAAAGAAAAGCTTCAAAAAATCCTCATCAGGGTGTCTTCTATATTAAGCGAACATGCAAAAGTGAG
CGGATTCTCTCCGGTTGGACTTGAGTTAGGTTTTGGAGGTCAAGGACCTCTGCCGCCGTTTACTTTTCAATTAAAAAACG
GCTGCACGATGGAGCTTGTTGGAAGGATTGACCGAGTGGATAAAGCAGAAGGGTCAAAAGGACTTCTATTAAGAATTGTC
GACTATAAGTCCAGTGAGAAGGGGCTTGATCTAGCAGAAGTTTACTACGGTTTAGCCTTGCAAATGCTCACTTATTTAGA
CCTGACCATTACGTATTCTAAAGAATGGCTTGGCATGGAAGCGACACCGGCAGGAATTTTATACTTCCATATTCATGACC
CGCTGATTCAAGCGCCTATTCCGCTTGGAGAAGATGCAATTGAGCAGGAAATATTGAAGAAATTTAAAATGAAGGGTCTA
TTGCTCGAAGATGTGGAAGCAGTGAAGTTGATGGATCAAACACTCGAATCAGGAAGATCACAAGTCATCCAAGCTGGTTT
GAAAAAGGATGGTTCTTTCCGATCTGACTCTGCGGTATTAAGTGAAGATCATTTTCACATTCTCACAAAACACGTTCGGC
GAACCTTTGAAGAGGCAGGAGAAAGAATTACAAGCGGTGAGGTAGAGATAAACCCATATAAATTAAAGGATCAAACCCCT
TGCAGCTTTTGCTCCTTTAAGTCTATTTGCCAATTTGATGAATCAATAGAAGATAATGAATTTAGGGTGCTCACCTCTGA
GAAGGATGATGTCATGTTAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.494

99.316

0.66

Multiple sequence alignment