Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACJEH3_RS05760 Genome accession   NZ_CP174245
Coordinates   1294023..1294571 (-) Length   182 a.a.
NCBI ID   WP_408075995.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF544     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1289023..1299571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEH3_RS05740 (ACJEH3_05740) aroK 1289753..1290280 (-) 528 WP_010946667.1 shikimate kinase AroK -
  ACJEH3_RS05745 (ACJEH3_05745) pilQ 1290726..1292822 (-) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  ACJEH3_RS05750 (ACJEH3_05750) pilP 1292826..1293413 (-) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  ACJEH3_RS05755 (ACJEH3_05755) pilO 1293410..1294009 (-) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJEH3_RS05760 (ACJEH3_05760) pilN 1294023..1294571 (-) 549 WP_408075995.1 PilN domain-containing protein Machinery gene
  ACJEH3_RS05765 (ACJEH3_05765) pilM 1294575..1295639 (-) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  ACJEH3_RS05770 (ACJEH3_05770) ravI 1296056..1297066 (+) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 21249.00 Da        Isoelectric Point: 10.1404

>NTDB_id=1076516 ACJEH3_RS05760 WP_408075995.1 1294023..1294571(-) (pilN) [Legionella pneumophila strain SPF544]
MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVSHLISNQMNRNQILQKEITALDEQIKEIKALKQIREG
LISRISIVQNLQSTRTLMVHLFDELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
NVEKKQSPNNEFKLSFVLKPKY

Nucleotide


Download         Length: 549 bp        

>NTDB_id=1076516 ACJEH3_RS05760 WP_408075995.1 1294023..1294571(-) (pilN) [Legionella pneumophila strain SPF544]
ATGACTGAAATTAATTTATTGCCATGGCGTGAACAAAAACGTGAGCAGGAAAAGAAATTATTTACAACCATGCTATTGAT
AGGAATTATCATCGCTGCAGCAATTGTTTTTTTAATGAACTCTTATGTTTCTCATTTAATAAGTAATCAAATGAACCGAA
ATCAGATTTTACAAAAAGAAATTACAGCTTTAGATGAACAGATCAAGGAGATTAAAGCATTAAAACAAATCAGGGAAGGT
TTGATTTCAAGAATATCGATTGTGCAGAATCTGCAGTCAACAAGAACTTTAATGGTTCACTTATTTGATGAATTAATAAA
AATTACTCCTCCAGGAGTTTACATAACCAAGATGCAAAGAGAACACGATGTAGTAACACTCTGGGGGTATTCCGAATCGA
ATACAAGTATTTCTATTTTGATGAGAAATATAGAGGCTAATGTCTGGATACAGAACCCTGTTCTAACTGAAATTAAAAAA
AATGTTGAAAAAAAACAGTCTCCAAACAATGAATTTAAATTAAGTTTTGTGCTTAAACCCAAATATTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

98.901

100

0.989

Multiple sequence alignment