Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACJEH1_RS04610 Genome accession   NZ_CP174226
Coordinates   1015798..1016346 (+) Length   182 a.a.
NCBI ID   WP_010946663.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF640     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1010798..1021346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEH1_RS04600 (ACJEH1_04600) ravI 1013303..1014313 (-) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -
  ACJEH1_RS04605 (ACJEH1_04605) pilM 1014730..1015794 (+) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  ACJEH1_RS04610 (ACJEH1_04610) pilN 1015798..1016346 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  ACJEH1_RS04615 (ACJEH1_04615) pilO 1016360..1016959 (+) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJEH1_RS04620 (ACJEH1_04620) pilP 1016956..1017543 (+) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  ACJEH1_RS04625 (ACJEH1_04625) pilQ 1017547..1019643 (+) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  ACJEH1_RS04630 (ACJEH1_04630) aroK 1020089..1020616 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 21267.04 Da        Isoelectric Point: 10.1404

>NTDB_id=1075704 ACJEH1_RS04610 WP_010946663.1 1015798..1016346(+) (pilN) [Legionella pneumophila strain SPF640]
MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVSHLISNQMNRNQILQKEITALDEQIKEIKALKQIREG
LISRMSIVQNLQSTRTLMVHLFDELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
NVEKKQSPNNEFKLSFVLKPKY

Nucleotide


Download         Length: 549 bp        

>NTDB_id=1075704 ACJEH1_RS04610 WP_010946663.1 1015798..1016346(+) (pilN) [Legionella pneumophila strain SPF640]
ATGACTGAAATTAATTTATTGCCATGGCGTGAACAAAAACGTGAGCAGGAAAAGAAATTATTTACAACCATGCTATTGAT
AGGAATTATCATCGCTGCAGCAATTGTTTTTTTAATGAACTCTTATGTTTCTCATTTAATAAGTAATCAAATGAACCGAA
ATCAGATTTTACAAAAAGAAATTACAGCTTTAGATGAACAGATCAAGGAGATTAAAGCATTAAAACAAATCAGGGAAGGT
TTGATTTCAAGAATGTCGATTGTGCAGAATCTGCAGTCAACAAGAACTTTAATGGTTCACTTATTTGATGAATTAATAAA
AATTACTCCTCCAGGAGTTTACATAACCAAGATGCAAAGAGAACACGATGTAGTAACACTCTGGGGGTATTCCGAATCGA
ATACAAGTATTTCTATTTTGATGAGAAATATAGAGGCTAATGTCTGGATACAGAACCCTGTTCTAACTGAAATTAAAAAA
AATGTTGAAAAAAAACAGTCTCCAAACAATGAATTTAAATTAAGTTTTGTGCTTAAACCCAAATATTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

99.451

100

0.995

Multiple sequence alignment