Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACJEHQ_RS05765 Genome accession   NZ_CP174217
Coordinates   1294025..1294573 (-) Length   182 a.a.
NCBI ID   WP_010946663.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF651     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1289025..1299573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEHQ_RS05745 (ACJEHQ_05745) aroK 1289755..1290282 (-) 528 WP_010946667.1 shikimate kinase AroK -
  ACJEHQ_RS05750 (ACJEHQ_05750) pilQ 1290728..1292824 (-) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  ACJEHQ_RS05755 (ACJEHQ_05755) pilP 1292828..1293415 (-) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  ACJEHQ_RS05760 (ACJEHQ_05760) pilO 1293412..1294011 (-) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJEHQ_RS05765 (ACJEHQ_05765) pilN 1294025..1294573 (-) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  ACJEHQ_RS05770 (ACJEHQ_05770) pilM 1294577..1295641 (-) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  ACJEHQ_RS05775 (ACJEHQ_05775) ravI 1296058..1297068 (+) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 21267.04 Da        Isoelectric Point: 10.1404

>NTDB_id=1075340 ACJEHQ_RS05765 WP_010946663.1 1294025..1294573(-) (pilN) [Legionella pneumophila strain SPF651]
MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVSHLISNQMNRNQILQKEITALDEQIKEIKALKQIREG
LISRMSIVQNLQSTRTLMVHLFDELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
NVEKKQSPNNEFKLSFVLKPKY

Nucleotide


Download         Length: 549 bp        

>NTDB_id=1075340 ACJEHQ_RS05765 WP_010946663.1 1294025..1294573(-) (pilN) [Legionella pneumophila strain SPF651]
ATGACTGAAATTAATTTATTGCCATGGCGTGAACAAAAACGTGAGCAGGAAAAGAAATTATTTACAACCATGCTATTGAT
AGGAATTATCATCGCTGCAGCAATTGTTTTTTTAATGAACTCTTATGTTTCTCATTTAATAAGTAATCAAATGAACCGAA
ATCAGATTTTACAAAAAGAAATTACAGCTTTAGATGAACAGATCAAGGAGATTAAAGCATTAAAACAAATCAGGGAAGGT
TTGATTTCAAGAATGTCGATTGTGCAGAATCTGCAGTCAACAAGAACTTTAATGGTTCACTTATTTGATGAATTAATAAA
AATTACTCCTCCAGGAGTTTACATAACCAAGATGCAAAGAGAACACGATGTAGTAACACTCTGGGGGTATTCCGAATCGA
ATACAAGTATTTCTATTTTGATGAGAAATATAGAGGCTAATGTCTGGATACAGAACCCTGTTCTAACTGAAATTAAAAAA
AATGTTGAAAAAAAACAGTCTCCAAACAATGAATTTAAATTAAGTTTTGTGCTTAAACCCAAATATTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

99.451

100

0.995

Multiple sequence alignment