Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACH2FV_RS12435 Genome accession   NZ_CP172339
Coordinates   2421790..2424114 (-) Length   774 a.a.
NCBI ID   WP_395893921.1    Uniprot ID   -
Organism   Bacillus safensis subsp. safensis strain KGU003     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2416790..2429114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH2FV_RS12420 (ACH2FV_12420) dtd 2418439..2418882 (-) 444 WP_024427859.1 D-aminoacyl-tRNA deacylase -
  ACH2FV_RS12425 (ACH2FV_12425) - 2418901..2421111 (-) 2211 WP_024427858.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  ACH2FV_RS12430 (ACH2FV_12430) - 2421254..2421766 (-) 513 WP_024423133.1 adenine phosphoribosyltransferase -
  ACH2FV_RS12435 (ACH2FV_12435) recJ 2421790..2424114 (-) 2325 WP_395893921.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACH2FV_RS12440 (ACH2FV_12440) - 2424196..2424546 (-) 351 WP_395893922.1 lipopolysaccharide assembly LapA domain-containing protein -
  ACH2FV_RS12445 (ACH2FV_12445) - 2424648..2425136 (-) 489 WP_395893923.1 cation:proton antiporter regulatory subunit -
  ACH2FV_RS12450 (ACH2FV_12450) secDF 2425519..2427747 (-) 2229 WP_041107052.1 protein translocase subunit SecDF -
  ACH2FV_RS12455 (ACH2FV_12455) - 2427789..2428085 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 86993.57 Da        Isoelectric Point: 4.9131

>NTDB_id=1066520 ACH2FV_RS12435 WP_395893921.1 2421790..2424114(-) (recJ) [Bacillus safensis subsp. safensis strain KGU003]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGELPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEASEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVSLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVQAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDTLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDLPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMQLAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=1066520 ACH2FV_RS12435 WP_395893921.1 2421790..2424114(-) (recJ) [Bacillus safensis subsp. safensis strain KGU003]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGACCTAATGAACAGGCTTTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTTATCACGGTCGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCCATTGTCCATCCTAAGCA
GCCTGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGTGTCGCGTTTAAAGTGGCGCATGCACTTCTTGGAGAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACCATCGCGGATCTCGTACCGCTTCATGATGAGAATAGATGGCTC
GCCAAAAAAGGGCTTATGCAGCTTCGGCAGTCGAATAGACCGGGTCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCAAGCGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCAGATC
CAGCTGTTCATCTTCTTATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCAGGCGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTAACGGATACTTTTTCTCGTCCGGCGA
TTGTACTTGGAATAGATGAAGAAACGCAAATGGCAAAAGGATCAGCCCGGAGCATTCCTGGCTTCGATCTATTCTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
ATCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATTCAGCGGGTCG
ATGCCGTGTGCAAGGTAGACGAATTGACGGTTCAAGCGATTGAAGAAGCGGGGATGCTGTCACCATTTGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTGGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACACACTGCTTGACTGTGTTGGCTTTCACCAAGGGAAATTAAAAGAGGAAATTGTTCCAGGAA
GCCGCATTTCTGTGATTGGAGAAATGTCTGTCAATGAATGGAATAACCGGAAGAAGCCCCAGCTGATGTTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACACTTGATTCAGAAAA
ATCATTGGTTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATCTCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACATTTGATGTACAAGGAAAGTATGTGGTCTTTGCAGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCGAGAATTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCTTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGCAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCGTCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(311-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.31

100

0.676

Multiple sequence alignment