Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACG4IW_RS05735 Genome accession   NZ_CP172327
Coordinates   1249688..1250704 (-) Length   338 a.a.
NCBI ID   WP_000520444.1    Uniprot ID   A0A0F0B833
Organism   Vibrio cholerae strain M650     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1244688..1255704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG4IW_RS05710 aroK 1245571..1246095 (-) 525 WP_000818616.1 shikimate kinase AroK -
  ACG4IW_RS05715 pilQ 1246282..1247997 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  ACG4IW_RS05720 pilP 1248023..1248541 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  ACG4IW_RS05725 pilO 1248531..1249121 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACG4IW_RS05730 pilN 1249114..1249698 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  ACG4IW_RS05735 pilM 1249688..1250704 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  ACG4IW_RS05740 - 1250847..1253324 (+) 2478 WP_000668733.1 penicillin-binding protein 1A -
  ACG4IW_RS05745 oxyR 1253398..1254291 (-) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  ACG4IW_RS05750 - 1254431..1255162 (+) 732 WP_000495223.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37707.44 Da        Isoelectric Point: 7.3328

>NTDB_id=1066406 ACG4IW_RS05735 WP_000520444.1 1249688..1250704(-) (pilM) [Vibrio cholerae strain M650]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1066406 ACG4IW_RS05735 WP_000520444.1 1249688..1250704(-) (pilM) [Vibrio cholerae strain M650]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F0B833

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

100

100

1
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment