Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   ACG4IU_RS12250 Genome accession   NZ_CP171664
Coordinates   2688004..2688864 (+) Length   286 a.a.
NCBI ID   WP_394861253.1    Uniprot ID   -
Organism   Vibrio cholerae strain M813     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2683004..2693864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG4IU_RS12230 greB 2683532..2684017 (-) 486 WP_000850496.1 transcription elongation factor GreB -
  ACG4IU_RS12235 - 2684262..2686583 (+) 2322 WP_000030087.1 Tex family protein -
  ACG4IU_RS12240 - 2686675..2687145 (-) 471 WP_000961392.1 hypothetical protein -
  ACG4IU_RS12245 bioH 2687307..2688068 (-) 762 WP_001240449.1 pimeloyl-ACP methyl ester esterase BioH -
  ACG4IU_RS12250 comF 2688004..2688864 (+) 861 WP_394861253.1 phosphoribosyltransferase family protein Machinery gene
  ACG4IU_RS12255 nfuA 2688952..2689539 (+) 588 WP_000070178.1 Fe-S biogenesis protein NfuA -
  ACG4IU_RS12260 nudE 2689678..2690226 (+) 549 WP_000095812.1 ADP compounds hydrolase NudE -
  ACG4IU_RS12265 cysQ 2690249..2691076 (+) 828 WP_000106799.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  ACG4IU_RS12270 - 2691128..2691886 (-) 759 WP_000817146.1 type II secretion system protein N -
  ACG4IU_RS12275 - 2691888..2692385 (-) 498 WP_000661339.1 type II secretion system protein M -
  ACG4IU_RS12280 gspL 2692395..2693606 (-) 1212 WP_001288747.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32544.53 Da        Isoelectric Point: 8.2656

>NTDB_id=1062744 ACG4IU_RS12250 WP_394861253.1 2688004..2688864(+) (comF) [Vibrio cholerae strain M813]
MPQPCTNTRSCPCPLTCQYNAMVTVSPSISIFIHSANWQDEAHYPDSWLAMLTHWLRKTTAPLLTPECHLCRLALDTNSP
FGVCSACQAWLEHGYRCARCGLPTLTPVDQCGQCLGQPPPWRKLMCVGDYRFPLSDAVHQLKYQRQFWQAPRLAKLLATQ
INEPAPLLCSVPLHWRRRWQRGFNQSDLLARELANMLNVEYDHQLFARRRATPHQQGLSKAQRIHNLRDAFVLNHPPNQP
HVAIVDDVVTTGSTIRHLCDLLLDVGVQSIDIYCICRTPKPKDSHG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1062744 ACG4IU_RS12250 WP_394861253.1 2688004..2688864(+) (comF) [Vibrio cholerae strain M813]
ATGCCCCAGCCGTGTACCAATACCAGATCTTGCCCTTGTCCACTGACTTGCCAATACAACGCCATGGTCACAGTTTCTCC
GTCTATTTCCATCTTCATCCACTCTGCCAATTGGCAAGATGAAGCACATTACCCCGATTCTTGGCTCGCTATGTTAACCC
ATTGGCTCAGAAAAACTACCGCACCACTGCTGACTCCGGAATGTCATCTCTGTCGACTGGCGCTAGATACAAATTCGCCG
TTTGGCGTCTGCTCTGCCTGCCAAGCTTGGCTTGAGCACGGTTATCGCTGCGCACGCTGCGGTTTACCGACTCTTACCCC
AGTTGATCAGTGCGGGCAGTGTTTGGGTCAGCCTCCGCCGTGGCGAAAACTGATGTGTGTCGGCGATTACCGCTTTCCAC
TTAGTGATGCGGTGCATCAACTCAAATACCAACGCCAGTTTTGGCAAGCGCCGCGCTTGGCTAAGCTACTCGCCACCCAG
ATTAACGAGCCAGCCCCGTTGCTGTGCAGCGTGCCACTGCATTGGCGGCGACGCTGGCAACGCGGCTTTAATCAAAGCGA
TCTGCTGGCGCGCGAGTTAGCCAATATGCTGAACGTTGAATATGACCACCAACTCTTTGCACGCCGCCGAGCGACACCGC
ATCAACAAGGACTCAGCAAGGCGCAGCGAATCCACAACCTACGCGATGCGTTTGTGCTGAATCATCCGCCAAACCAACCA
CATGTTGCGATTGTGGACGATGTGGTGACCACAGGCAGTACGATCCGACATTTATGCGATTTACTGCTTGATGTCGGTGT
GCAAAGCATTGATATTTACTGCATATGCCGCACTCCCAAGCCGAAAGATAGCCACGGTTGA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(189-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Vibrio cholerae strain A1552

99.65

100

0.997
  comF Vibrio campbellii strain DS40M4

47.059

83.217

0.392

Multiple sequence alignment