Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACFW99_RS13210 Genome accession   NZ_CP170651
Coordinates   2705935..2708295 (-) Length   786 a.a.
NCBI ID   WP_317859505.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2700935..2713295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS13190 (ACFW99_13190) - 2701010..2702572 (+) 1563 WP_015417910.1 SH3 domain-containing protein -
  ACFW99_RS13195 (ACFW99_13195) dtd 2702599..2703042 (-) 444 WP_077392147.1 D-aminoacyl-tRNA deacylase -
  ACFW99_RS13200 (ACFW99_13200) relA 2703055..2705259 (-) 2205 WP_003152716.1 GTP diphosphokinase -
  ACFW99_RS13205 (ACFW99_13205) - 2705417..2705929 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  ACFW99_RS13210 (ACFW99_13210) recJ 2705935..2708295 (-) 2361 WP_317859505.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACFW99_RS13215 (ACFW99_13215) - 2708351..2708677 (-) 327 WP_015417912.1 lipopolysaccharide assembly LapA domain-containing protein -
  ACFW99_RS13220 (ACFW99_13220) - 2708741..2709238 (-) 498 WP_044801628.1 cation:proton antiporter regulatory subunit -
  ACFW99_RS13225 (ACFW99_13225) secDF 2709370..2711589 (-) 2220 WP_021494384.1 protein translocase subunit SecDF -
  ACFW99_RS13230 (ACFW99_13230) - 2711626..2711922 (-) 297 WP_021494385.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87281.72 Da        Isoelectric Point: 5.0685

>NTDB_id=1058427 ACFW99_RS13210 WP_317859505.1 2705935..2708295(-) (recJ) [Bacillus amyloliquefaciens strain JP5025]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSQG
EPIMIYGDYDADGVTSTSVMLTALRQLSADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPETMLDLAAIGTVADLVPLHDENRLI
ASLGIKRLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVAKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCVGFNKGELEAGVVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLISFNEKTAQKLRAKELSGELAGITSESEAEALQLDGKYAVFLDVPPSADR
LQHLLDGKKPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGAFDFGKHGSELARHRGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=1058427 ACFW99_RS13210 WP_317859505.1 2705935..2708295(-) (recJ) [Bacillus amyloliquefaciens strain JP5025]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAACTGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCGGTGATGCTGACGGCACTCAGACAGCT
GTCGGCGGACGTTGACTTTTATATTCCCGACCGTTTCACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGCTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTTGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGCTGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAACTATGCTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCTCTTCATGATGAAAACAGATTAATT
GCGTCACTCGGAATCAAAAGGCTTAGGACGACAAATCGGCCGGGCCTCAAAGCCCTGATTAAGCTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATCGGATTTCAGGTCGCGCCGAGGCTCAATGCCGTCGGAAGAATAGAACAGGCCGATC
CCGCCGTCCATCTGCTGATGACCGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGGCAAAAGCCGGCTGGAATCCGGGAGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGGCCCGCTG
TTGTGCTTGGGATAGATGAGGAGCAGGGAATCGCTAAAGGGTCGGCGCGAAGCATCAGAGGCTTCAACCTGTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCAGGAATGACGTTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTTACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTAGAAGACATAACCGTTGAAAGCATTACAGAATTAAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTGCTTGATGATGTGCGCCTGATCGGAGCGAATAAAAATCACGTGAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCGTCGGCTTTAACAAAGGAGAGCTTGAAGCCGGAGTTGTCCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAGCCCCAGCTGATGATTAAAGACGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCAGGGGCAAGCGGACGTGGGAAGACACGGTCACCGCGCTGCCGAAGGAAAA
ACGGGTGCTCATCAGCTTTAACGAAAAAACCGCTCAGAAGCTCCGTGCAAAAGAGCTGTCGGGCGAGCTGGCCGGGATCA
CATCGGAATCCGAGGCGGAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCATTTGCTTGACGGAAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTCCTGTCCTC
ATTTCCATCTCGGAATGATTTCAAGTGGTTTTACGCATTTCTCTTGAAAAGGGGCGCTTTTGACTTCGGCAAGCATGGTT
CAGAGCTTGCGAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGCGCGAAAAAACGCGACCTGGCAGATTCGCAAACCTATCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCCATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAAGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(315-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

81.934

100

0.819

Multiple sequence alignment