Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABGU63_RS01305 Genome accession   NZ_OZ040267
Coordinates   281633..283129 (+) Length   498 a.a.
NCBI ID   WP_001040681.1    Uniprot ID   -
Organism   Escherichia coli isolate 30821_1#88     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 276633..288129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABGU63_RS01275 tusA 276880..277125 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  ABGU63_RS01280 zntA 277227..279425 (-) 2199 WP_000106599.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  ABGU63_RS01285 yhhN 279499..280125 (-) 627 WP_000964718.1 lysoplasmalogenase -
  ABGU63_RS01290 yhhM 280266..280625 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  ABGU63_RS01295 yhhL 280628..280897 (-) 270 WP_001295207.1 DUF1145 family protein -
  ABGU63_RS01300 rsmD 280887..281483 (-) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  ABGU63_RS01305 pilA 281633..283129 (+) 1497 WP_001040681.1 signal recognition particle-docking protein FtsY Machinery gene
  ABGU63_RS01310 ftsE 283132..283800 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  ABGU63_RS01315 ftsX 283793..284851 (+) 1059 WP_001042013.1 permease-like cell division protein FtsX -
  ABGU63_RS01320 rpoH 285096..285950 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  ABGU63_RS01325 livJ 286221..287324 (+) 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  ABGU63_RS01330 ataR 287473..287739 (+) 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54672.49 Da        Isoelectric Point: 4.1449

>NTDB_id=1049999 ABGU63_RS01305 WP_001040681.1 281633..283129(+) (pilA) [Escherichia coli isolate 30821_1#88]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=1049999 ABGU63_RS01305 WP_001040681.1 281633..283129(+) (pilA) [Escherichia coli isolate 30821_1#88]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGAGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGATCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACAGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCTGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment