Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   ABGU81_RS01230 Genome accession   NZ_OZ039398
Coordinates   268109..268873 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli isolate 30348_2#256     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 263109..273873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABGU81_RS01200 - 263743..265101 (-) 1359 WP_001525857.1 PTS galactitol transporter subunit IIC -
  ABGU81_RS01205 - 265178..265459 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  ABGU81_RS01210 - 265456..265929 (-) 474 WP_001161648.1 PTS sugar transporter subunit IIA -
  ABGU81_RS01215 - 265954..266700 (-) 747 WP_001296489.1 GntR family transcriptional regulator -
  ABGU81_RS01220 nikR 266899..267300 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  ABGU81_RS01225 nikE 267306..268112 (-) 807 WP_000173684.1 nickel import ATP-binding protein NikE -
  ABGU81_RS01230 amiE 268109..268873 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  ABGU81_RS01235 nikC 268873..269706 (-) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  ABGU81_RS01240 nikB 269703..270647 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  ABGU81_RS01245 nikA 270647..272221 (-) 1575 WP_000493124.1 nickel ABC transporter substrate-binding protein -
  ABGU81_RS01250 acpT 272332..272919 (-) 588 WP_000285760.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=1048965 ABGU81_RS01230 WP_001136232.1 268109..268873(-) (amiE) [Escherichia coli isolate 30348_2#256]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=1048965 ABGU81_RS01230 WP_001136232.1 268109..268873(-) (amiE) [Escherichia coli isolate 30348_2#256]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGCATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTAGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCGCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment