Detailed information NTG1043232

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACEN24_RS07920
• Genome accession: NZ_CP168224
• Coordinates: 1647916..1648413 (-)
• Length: 165 a.a.
• NCBI ID: WP_001794055.1
• Uniprot ID: A0A7U7EV69
• Organism: Staphylococcus aureus strain C62
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1642916..1653413

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEN24_RS07895 (ACEN24_07895)	gcvPB	1643078..1644550 (-)	1473	WP_000202178.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ACEN24_RS07900 (ACEN24_07900)	gcvPA	1644543..1645889 (-)	1347	WP_000019697.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACEN24_RS07905 (ACEN24_07905)	gcvT	1645909..1647000 (-)	1092	WP_000093347.1	glycine cleavage system aminomethyltransferase GcvT	-
ACEN24_RS07910 (ACEN24_07910)	-	1647159..1647683 (-)	525	WP_001015125.1	shikimate kinase	-
ACEN24_RS07915 (ACEN24_07915)	-	1647673..1647819 (-)	147	WP_001789879.1	hypothetical protein	-
ACEN24_RS07920 (ACEN24_07920)	comGF	1647916..1648413 (-)	498	WP_001794055.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACEN24_RS07925 (ACEN24_07925)	comGE	1648331..1648630 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACEN24_RS07930 (ACEN24_07930)	comGD	1648617..1649063 (-)	447	WP_001791207.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACEN24_RS07935 (ACEN24_07935)	comGC	1649041..1649352 (-)	312	WP_000472257.1	competence type IV pilus major pilin ComGC	Machinery gene
ACEN24_RS07940 (ACEN24_07940)	comGB	1649366..1650436 (-)	1071	WP_000776412.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACEN24_RS07945 (ACEN24_07945)	comGA	1650408..1651382 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
ACEN24_RS07950 (ACEN24_07950)	-	1651434..1652057 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACEN24_RS07955 (ACEN24_07955)	-	1652054..1652383 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACEN24_RS07960 (ACEN24_07960)	-	1652383..1653369 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19224.98 Da
• Isoelectric Point: 10.2192

>NTDB_id=1043232 ACEN24_RS07920 WP_001794055.1 1647916..1648413(-) (comGF) [Staphylococcus aureus strain C62]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1043232 ACEN24_RS07920 WP_001794055.1 1647916..1648413(-) (comGF) [Staphylococcus aureus strain C62]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAACAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A7U7EV69

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97