Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   ACDW36_RS25760 Genome accession   NZ_CP167984
Coordinates   5502085..5503422 (+) Length   445 a.a.
NCBI ID   WP_003110427.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 0304d2     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5497085..5508422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW36_RS25735 (ACDW36_25735) pgeF 5497228..5497956 (-) 729 WP_003094684.1 peptidoglycan editing factor PgeF -
  ACDW36_RS25740 (ACDW36_25740) rluD 5497953..5498915 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACDW36_RS25745 (ACDW36_25745) - 5499061..5500086 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  ACDW36_RS25750 (ACDW36_25750) - 5500243..5500488 (+) 246 WP_014603563.1 PP0621 family protein -
  ACDW36_RS25755 (ACDW36_25755) pilS 5500478..5502070 (+) 1593 WP_003110428.1 two-component system sensor histidine kinase PilS Regulator
  ACDW36_RS25760 (ACDW36_25760) pilR 5502085..5503422 (+) 1338 WP_003110427.1 two-component system response regulator PilR Regulator
  ACDW36_RS25765 (ACDW36_25765) thiO 5503468..5504562 (-) 1095 WP_023096126.1 glycine oxidase ThiO -
  ACDW36_RS25770 (ACDW36_25770) - 5504697..5505206 (+) 510 WP_009316266.1 Tfp pilus assembly protein FimT/FimU -
  ACDW36_RS25775 (ACDW36_25775) - 5505312..5505818 (+) 507 WP_003110423.1 Tfp pilus assembly protein FimT/FimU -
  ACDW36_RS25780 (ACDW36_25780) pilV 5505809..5506366 (+) 558 WP_003094697.1 type 4a pilus minor pilin PilV Machinery gene
  ACDW36_RS25785 (ACDW36_25785) pilW 5506363..5507187 (+) 825 WP_023096127.1 type 4a pilus minor pilin PilW -
  ACDW36_RS25790 (ACDW36_25790) pilX 5507184..5507771 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49723.03 Da        Isoelectric Point: 5.8638

>NTDB_id=1040795 ACDW36_RS25760 WP_003110427.1 5502085..5503422(+) (pilR) [Pseudomonas aeruginosa strain 0304d2]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLADRI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=1040795 ACDW36_RS25760 WP_003110427.1 5502085..5503422(+) (pilR) [Pseudomonas aeruginosa strain 0304d2]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTAGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGTGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGATCGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAAAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGATCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCGGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGTTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment