Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DB825_RS18495 Genome accession   NZ_CP162103
Coordinates   4265974..4267107 (+) Length   377 a.a.
NCBI ID   WP_008576907.1    Uniprot ID   Q3BP52
Organism   Xanthomonas perforans strain GEV2048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4260974..4272107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DB825_RS18470 (DB825_018470) - 4261133..4262329 (-) 1197 WP_008576912.1 cystathionine gamma-synthase -
  DB825_RS18475 (DB825_018475) - 4262429..4263799 (-) 1371 WP_008576911.1 pyridoxal-phosphate dependent enzyme -
  DB825_RS18480 (DB825_018480) - 4264035..4264253 (-) 219 WP_003484103.1 YdcH family protein -
  DB825_RS18485 (DB825_018485) - 4264605..4265495 (-) 891 WP_008576909.1 membrane protein -
  DB825_RS18490 (DB825_018490) - 4265492..4265875 (-) 384 WP_033479204.1 DUF4398 domain-containing protein -
  DB825_RS18495 (DB825_018495) pilU 4265974..4267107 (+) 1134 WP_008576907.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DB825_RS18500 (DB825_018500) maiA 4267505..4268167 (-) 663 WP_008576906.1 maleylacetoacetate isomerase -
  DB825_RS18505 (DB825_018505) - 4268199..4269257 (-) 1059 WP_171309826.1 fumarylacetoacetate hydrolase family protein -
  DB825_RS18510 (DB825_018510) - 4269566..4270954 (+) 1389 WP_008576904.1 DEAD/DEAH box helicase -
  DB825_RS18515 (DB825_018515) queD 4271090..4271443 (+) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  DB825_RS18520 (DB825_018520) - 4271636..4271971 (+) 336 WP_008576902.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41692.04 Da        Isoelectric Point: 6.1401

>NTDB_id=1024086 DB825_RS18495 WP_008576907.1 4265974..4267107(+) (pilU) [Xanthomonas perforans strain GEV2048]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEAAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1024086 DB825_RS18495 WP_008576907.1 4265974..4267107(+) (pilU) [Xanthomonas perforans strain GEV2048]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCCTACTCGT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAACGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGCCGT
TTCCGCGTGAACGTTTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATTCGCGCCATTCGCAGCCGGATTCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGTTCGACCGGTT
CGGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCAGTGGTCTCCCAGCG
CCTGGTCAAGGGCGTGGACGGGCGCCGCCGCCCCGCCACCGAGGTGTTGCTCAATACCCCGATGATCCGCGACCTGCTAC
GGCGCGGCCAGGTGCACGAGGTCAAGGCAGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTGAAGTCGGGCGAAATCGAGCAGGAAGAAGCACTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGTCTCTCCGAGGGCGGCTCCGGCGAGCACGACCCGTATGCGGATTACGAAGCGGCCGCGGCACCGAAGATCAGTC
ACGGGTTCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3BP52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.444

95.491

0.472
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.617

88.594

0.369
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Vibrio cholerae strain A1552

41.141

88.329

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.329

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.042

0.361
  pilT Neisseria meningitidis 8013

39.193

92.042

0.361

Multiple sequence alignment