Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AB3G27_RS05270 Genome accession   NZ_CP162095
Coordinates   1086553..1090062 (+) Length   1169 a.a.
NCBI ID   WP_368198160.1    Uniprot ID   -
Organism   Bacillus pumilus strain LBUM494     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1081553..1095062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3G27_RS05240 (AB3G27_05240) lepB 1082063..1082575 (+) 513 WP_041815362.1 signal peptidase I -
  AB3G27_RS05245 (AB3G27_05245) - 1082610..1083755 (-) 1146 WP_368198534.1 AbrB family transcriptional regulator -
  AB3G27_RS05250 (AB3G27_05250) - 1084022..1084444 (+) 423 WP_003210872.1 BlaI/MecI/CopY family transcriptional regulator -
  AB3G27_RS05255 (AB3G27_05255) - 1084437..1085276 (+) 840 WP_050945367.1 M56 family metallopeptidase -
  AB3G27_RS05260 (AB3G27_05260) - 1085333..1085743 (+) 411 WP_106029930.1 DoxX family protein -
  AB3G27_RS05265 (AB3G27_05265) - 1085941..1086387 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  AB3G27_RS05270 (AB3G27_05270) addB 1086553..1090062 (+) 3510 WP_368198160.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB3G27_RS05275 (AB3G27_05275) addA 1090040..1093738 (+) 3699 WP_368198161.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB3G27_RS05280 (AB3G27_05280) sbcD 1093765..1094940 (+) 1176 WP_044139868.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134824.76 Da        Isoelectric Point: 5.3504

>NTDB_id=1023971 AB3G27_RS05270 WP_368198160.1 1086553..1090062(+) (addB) [Bacillus pumilus strain LBUM494]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLSQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLYAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYKLAKECGADISENIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSQFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
IAPPLYQLQKRLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDDEQLKFVANEH
VSLSYTISQLQQWLNQYPISGVWWSVYNYLMTSPHRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKLSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=1023971 AB3G27_RS05270 WP_368198160.1 1086553..1090062(+) (addB) [Bacillus pumilus strain LBUM494]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGTCTTGCTTGGTCGATTTTACAGCAGACTGGT
GGAGCGAATCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCAGAGGATATTGAGAAGATTTCAGCGTCTAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTTCTTTCCCAGCAAATTCCTTCAGCTGAAGAAATAAAAGGAGCACACATCTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCTTGCTGTATGCAGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATAAGCTTGCTAA
AGAGTGCGGAGCTGACATTTCTGAAAATATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCCGTTCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATCCTTGATTTAGTGAGAGAAAAAGGACTCAGACTGCGAGATATCTCGAT
TGTAGCGCGGCATGTAGATGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTAATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTTGAAAACTATTGCATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCAGTTTCATTACAGGCGTT
TCCAATCATTAGATGAGGATTTCAGGCAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGCGCCCCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAAAGCGCAAAAGGTAAGGGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCCTTATTCCAACAGATGATAGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATTGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTACGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGTCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGAGAATGGTTAAAACGAGCAGGAGCAGAGCTAGCA
GAGACAGGAAAAGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCGTCATTTGTATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAACAGGCTCTTTATGTGTTAGATCCAGAACAACTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACCATCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATCAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTCATCGGGATGTATCAAAAAACATCATGTCAAGCTTGTTCTTTACAAATCGAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAGTTCAATGCG
TGTGCATTCTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATCGG
CCAATTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTTCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAATTGAG
TGGATTTTCTCCGGTAGGGCTTGAGTTAGGTTTTGGAGGTCAAGGGCCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTTGTTGGAAGGATTGACAGGGTAGATAAGGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCACTTGCAGAGGATGAAATTGAGCAGGAAATCTTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCGCAAGTCATTCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCAGTTTTAAGTGAGGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.15

99.316

0.657

Multiple sequence alignment